Abstract: The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.

Abstract: Methods, compositions, and apparatuses for the identification of binding moieties that bind to targets are provided. Vectors encoding potential binding moieties are also provided. In certain embodiments, methods are provided for the presentation of potential binding moieties by cells, the selection of binding moieties that bind targets, and the amplification of the nucleic acids encoding the binding moieties.

Abstract: The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.

Abstract: A method is provided for binding a predetermined amount of a nucleic acid. The method involves contacting one or more sample solutions comprising a nucleic acid with a multiplicity of solid substrate binding units, where the solid substrate binding units bind the nucleic acid. Each binding unit has a predetermined binding capacity for the nucleic acid. An apparatus for binding one or more predetermined amounts of a nucleic acid is also provided.

Abstract: The present invention relates to methods for detecting the presence of one or more analytes of interest in a sample by measuring changes in fluorescence anisotropy as a result of binding of the analytes to specific aptamers. The aptamers are immobilized on a solid support and may be in the form of an array.

Abstract: A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture.

Abstract: Methods, compositions and kits are disclosed that utilize heteroconfigurational polynucleotide comprising a D-form polynucleotide sequence portion and an L-form polynucleotide sequence portion that is covalently linked to the D-form polynucleotide sequence portion.

Abstract: The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.

Abstract: The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.

Abstract: A contoured pan liner and method of making a pan liner is provided, the pan liner including a first liner side panel having a first upper edge; a second liner side panel having a second upper edge; and at least one seam joining the first and second side panels, the first side panel, second side panel and at least one seam forming an interior region having an opening along the first and second upper edges, and wherein at least a portion of the at least one seam defines an arcuate path along at least a lower portion of the interior region.

Abstract: A purified thermostable enzyme is derived from the eubacterium Thermosipho africanus. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' and/or 3'.fwdarw.5' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

Abstract: A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

Abstract: A purified thermostable enzyme is derived from the eubacterium Thermus species Z05. The enzyme has DNA polymerase, activity reverse transcriptase activity, and optionally 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and may be used with primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

Abstract: A purified thermostable enzyme is derived from the bacterium Thermus species sps17. The enzyme has DNA polymerase activity, reverse transcriptase activity, and optionally, 5'.fwdarw.3' exonuclease activity. The enzyme can be native or recombinant, and can be used with selected primers and nucleoside triphosphates in a temperature-cycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.

Abstract: A bag pack and a holder comprises a layered stack of flexible thermo-plastic bags having a folded over stiff header with oppositely projecting ears at its ends. The ears enter beneath flaps at opposite ends of a holder when bent rearwardly and inwardly. A central flap on the holder retains the header against forward pivotal movement as might dislodge the ears and the individual bags have exposed top openings when so arranged for successive removal from the pack. A second embodiment includes flaps on a holder entered between front and rear portions of a header for retention of the bag pack.

Abstract: This invention discloses heterobifunctional coupling agents for making a wide array of molecular conjugates. The agents contain a sterically hindered thiol, linked through a spacer arm to a second group reactive toward nucleophiles such as 1.degree. and 2.degree. amines or reactive thiols that are present on biological and organic materials. The coupling agents are useful for making conjugates containing a sterically hindered linkage.

Abstract: Recombinant diphtheria toxin A fragment muteins which are enzymatically inactive but immunologically crossreactive with diphtheria toxin are disclosed. Intermediates and methods for preparing such proteins using recombinant techniques are also described.

Abstract: DNA sequences encoding proteins processable by secretion leaders in recombinant hosts are described. The DNA sequences encode the NH.sub.2 -terminal region of proteins that are cleaved from a secretion leader and may be secreted through the cell membrane and, if present, cell wall in some cases. Proteins encoded by the DNA sequence have an NH.sub.2 -terminal amino acid sequence conforming to a consensus amino acid sequence that is processable by the particular secretion leader. DNA sequences encoding a consensus amino acid sequence processable by the diphterhia toxin secretion leader are disclosed. A novel Pseudomonas exotoxin and CSF-1 having NH.sub.2 -terminal sequences conforming to a consensus sequence are exemplified.