Abstract: The present invention provides Photobacterium sp. JH-ISH-224 ?2-6-sialyltransferase Psp26ST variants and expression cassettes, vectors, and host cells for expressing the Psp26ST variants. Methods of synthesizing sialylated products are also described.

Abstract: An optical demultiplexing device includes an optics body, a mirror, optical filters, and opto-electronic detectors. The optics body has a fiber port configured to receive an end of an optical fiber, a reflective surface aligned with the fiber port, a substantially planar filter mounting surface, and a substantially planar mirror mounting surface. The filter mounting surface and the mirror mounting surface are parallel to one another and formed on the same side of the optics body as each other. The mirror is mounted on the mirror mounting surface, and the filters are mounted on the filter mounting surface. Each filter is transparent to a different wavelength and is interposed in an optical path between one of the opto-electronic detectors and the reflector.

Abstract: An electrostatic ion trap for mass analysis includes a first array of electrodes and a second array of electrodes, spaced from the first array of electrode. The first and second arrays of electrodes may be planar arrays formed by parallel strip electrodes or by concentric, circular or part-circular electrically conductive rings. The electrodes of the arrays are supplied with substantially the same pattern of voltage whereby the distribution of electrical potential in the space between the arrays is such as to reflect ions isochronously in a flight direction causing them to undergo periodic, oscillatory motion in the space, focused substantially mid-way between the arrays. Amplifier circuitry is used to detect image current having frequency components related to the mass-to-charge ratio of ions undergoing the periodic, oscillatory motion.

Abstract: A system and a method are disclosed for performing static timing analysis. Information describing a distorted input waveform is received by a static timing analyzer. A transition time of the distorted input waveform is determined. Based on the determined input transition time a nominal input waveform and a corresponding nominal output waveform are received. An input waveform distortion is computed based on the nominal input waveform and the distorted input waveform. An output waveform distortion is computed based on an augmented circuit and the input waveform distortion. A distorted output waveform is computed based on the nominal output waveform and the output waveform distortion. The waveforms are represented using the distortion values which are smaller than the actual waveform values, thereby allowing for compact representation. A time-shifted version of an uncoupled input waveform is used to perform conservative timing analysis of circuits that accounts for crosstalk in the circuit.

Abstract: The invention “Ion Trap Array (ITA)” pertains generally to the field of ion storage and analysis technologies, and particularly to the ion storing apparatus and mass spectrometry instruments which separate ions by its character such as mass-to-charge ratio. The aim of this invention is providing an apparatus for ion storage and analysis comprising at least two or more rows of parallel placed electrode array wherein each electrode array includes at least two or more parallel bar-shaped electrodes, by applying different phase of alternating current voltages on different bar electrodes to create alternating electric fields inside the space between two parallel electrodes of different rows of electrode arrays, multiple linear ion trapping fields paralleled constructed in the space between the different rows of electrode arrays which are open to adjacent each other without a real barrier.

Abstract: An embodiment of a method of testing software can include, as performed by at least one computing device, evaluating a first criterion for a plurality of software components, selecting a subset of the plurality of software components based on the evaluated first criterion, evaluating a second criterion for a plurality of test cases each defining a respective test to evaluate functionality of at least one of the software components, selecting a subset of the plurality of test cases based on the evaluated second criterion, and testing the selected subset of the plurality of software components utilizing the selected subset of the plurality of test cases.

Abstract: A method for modifying the refractive index of an optical polymeric material. The method comprises continuously irradiating predetermined regions of an optical, polymeric material with femtosecond laser pulses to form a gradient index refractive structure within the material. The optical polymeric material can include a photosensitizer to increase the photoefficiency of the two-photo process resulting in the formation of the observed refractive structures. An optical device includes an optical, polymeric lens material having an anterior surface and posterior surface and an optical axis intersecting the surfaces and at least one laser-modified, GRIN layer disposed between the anterior surface and the posterior surface and arranged along a first axis 45° to 90° to the optical axis.

Abstract: In a method for making an optical communications module, elements in the optical signal path are aligned relative to a lens mounting frame. The frame is attached to the surface of a printed circuit board. The frame bears fiducial markings. An opto-electronic device is then aligned relative to the frame using the fiducial markings. One or more bottom lens devices are aligned relative to the lens mounting frame using the fiducial markings. Finally, a top lens device is attached to the lens mounting frame over the bottom lens devices.

Abstract: A parallel optical communications module includes a top lens and a bottom lens that are spaced from one another to inhibit mechanical forces acting upon the top lens from being transferred to the bottom lens, which is optically aligned with an opto-electronic light source or light detector. The top lens has a reflector portion configured to redirect the optical signals between the bottom lens and one or more optical fibers.

Abstract: A circuit for generating a voltage waveform at an output node. The circuit includes a voltage rail connected to the output node via a voltage rail switch; an anchor node connected to the output node via an inductor and a bidirectional switch, wherein the bidirectional switch includes two or more transistors connected in series; and a control unit configured to change the voltage at the output node by controlling the voltage rail switch and the bidirectional switch so that, if a load capacitance is connected to the output node, a resonant circuit is established between the inductor and the load capacitance. The circuit may be included in an apparatus for use in processing charged particles, e.g. for use in performing mass spectrometry or ion mobility spectrometry.

Abstract: The present invention provides a method for preparing an ion optical device. A substrate is fabricated with a hard material adapted for a grinding process, the substrate at least including a planar surface, and including at least one insulating material layer. Next, one or more linear grooves are cut on the planar surface, to form multiple discrete ion optical electrode regions on the planar surface separated by the linear grooves. Then, conductive leads are fabricated on other substrate surfaces than the planar surface and in a through hole inside the substrate, to provide voltages required on ion optical electrodes. By using high-hardness materials in cooperation with high-precision machining, higher precision and a desired discrete electrode contour can be obtained.

Abstract: An electrostatic ion trap for mass analysis includes a first array of electrodes and a second array of electrodes, spaced from the first array of electrode. The first and second arrays of electrodes may be planar arrays formed by parallel strip electrodes or by concentric, circular or part-circular electrically conductive rings. The electrodes of the arrays are supplied with substantially the same pattern of voltage whereby the distribution of electrical potential in the space between the arrays is such as to reflect ions isochronously in a flight direction causing them to undergo periodic, oscillatory motion in the space, focused substantially mid-way between the arrays. Amplifier circuitry is used to detect image current having frequency components related to the mass-to-charge ratio of ions undergoing the periodic, oscillatory motion.

Abstract: A method for modifying the refractive index of an optical polymeric material. The method comprises continuously irradiating predetermined regions of an optical, polymeric material with femtosecond laser pulses to form a gradient index refractive structure within the material. An optical device includes an optical, polymeric lens material having an anterior surface and posterior surface and an optical axis intersecting the surfaces and at least one laser-modified, GRIN layer disposed between the anterior surface and the posterior surface and arranged along a first axis 45° to 90° to the optical axis, and further characterized by a variation in index of refraction across at least one of at least a portion of the adjacent segments and along each segment.

Abstract: The invention “Ion Trap Array (ITA)” pertains generally to the field of ion storage and analysis technologies, and particularly to the ion storing apparatus and mass spectrometry instruments which separate ions by its character such as mass-to-charge ratio. The aim of this invention is providing an apparatus for ion storage and analysis comprising at least two or more rows of parallel placed electrode array wherein each electrode array includes at least two or more parallel bar-shaped electrodes, by applying different phase of alternating current voltages on different bar electrodes to create alternating electric fields inside the space between two parallel electrodes of different rows of electrode arrays, multiple linear ion trapping fields paralleled constructed in the space between the different rows of electrode arrays which are open to adjacent each other without a real barrier.

Abstract: Embodiments are directed to identifying, by an apparatus comprising a processing device, a pattern in a graph that has a support value greater than a threshold, wherein: the graph comprises a plurality of weighted nodes coupled to one another by one or more weighted edges, and each of the plurality of nodes is associated with at least one weighted attribute.

Abstract: A tandem mass spectrometer is provided in the present invention. The mass spectrometer includes an ion source, a quadrupole mass filter located at downstream side of the ion source, a linear ion trap disposed at downstream side of the mass filter and an ion detector placed on the side of the ion trap, all of which are placed in a vacuum environment. The instrument can obtain MS meeting the standard spectral library search criteria by the quadrupole mass filter cooperating with linear ion trap, realize any multi-stage MS under two modes of axial collision and resonance excitation, and predict and optimize the inflow amount and types of samples under the ion trap analysis mode by the quadrupole. A tandem MS analysis method is also provided, which can repeatedly provide precursor ion selection, ion acceleration, achieve high-energy collision dissociation, low product ion mass discrimination effect.

Abstract: A method for modifying the refractive index of an optical, hydrogel polymeric material. The method comprises irradiating predetermined regions of an optical, polymeric material with a laser to form refractive structures. To facilitate the formation of the refractive structures the optical, hydrogel polymeric material comprises a photosensitizer. The presence of the photosensitizer permits one to set a scan rate to a value that is at least fifty times greater than a scan rate without the photosensitizer in the material, yet provides similar refractive structures in terms of the observed change in refractive index. Alternatively, the photosensitizer in the polymeric material permits one to set an average laser power to a value that is at least two times less than an average laser power without the photosensitizer in the material, yet provide similar refractive structures.

Abstract: An optical device comprising an optical hydrogel with select regions that have been irradiated with laser light having a pulse energy from 0.01 nJ to 50 nJ and a wavelength from 600 nm to 900 nm. The irradiated regions are characterized by a positive change in refractive index of from 0.01 to 0.06, and exhibit little or no scattering loss. The optical hydrogel is prepared with a hydrophilic monomer.

Abstract: A multilayer circuit board is provided, which includes multiple core boards stacked together. The core board includes an insulation layer and at least one conductor layer attached together. The conductor layer includes a circuit. The core board has at least one identification conductor disposed at an edge of at least one conductor layer. The identification conductor forms an identification pattern on a side surface of the core board along a stacking direction of the core boards. The identification patterns of the multiple core boards are different from each other on the side surface of the multilayer circuit board along the stacking direction of the core boards. A manufacturing method of the multilayer circuit board is further provided.

Abstract: An optical demultiplexing device includes an optics body, a minor, optical filters, and opto-electronic detectors. The optics body has a fiber port configured to receive an end of an optical fiber, a reflective surface aligned with the fiber port, a substantially planar filter mounting surface, and a substantially planar mirror mounting surface. The filter mounting surface and the minor mounting surface are parallel to one another and formed on the same side of the optics body as each other. The minor is mounted on the minor mounting surface, and the filters are mounted on the filter mounting surface. Each filter is transparent to a different wavelength and is interposed in an optical path between one of the opto-electronic detectors and the reflector.