Abstract: A pneumatic micropump is provided. The pneumatic micropump includes a fluidic channel layer, an upper substrate, a lower substrate, an upper membrane and a lower membrane. The fluidic channel includes a fluid inlet a reservoir, and a fluid outlet, wherein the fluid passes through the fluid inlet, the reservoir and the fluid outlet, successively. The upper substrate includes an upper pneumatic chamber facing the reservoir. The lower substrate includes a lower pneumatic chamber facing the reservoir. The upper membrane is disposed between the upper pneumatic chamber and the reservoir, and the lower membrane is disposed between the lower pneumatic chamber and the reservoir.

Abstract: A concentration method of dielectrophoretic particles includes: providing a fluid pipe structure, wherein the fluid pipe structure has a protrudent structure lateral protruding inwardly so as to form a line-like gate; making a fluid containing particles to be measured flow through the fluid pipe structure; and applying an electrical field through the line-like gate so as to produce a dielectrophoresis force to concentrate the particles to be measured.

Abstract: A detection chip is provided. The detection chip includes a substrate, an active reagent, a hydrophilic droplet and a lipophilic substance. The substrate includes a first containing slot, wherein the first containing slot includes a first space and a second space adjacent to each other. The active reagent is disposed in the first space of the first containing slot. The hydrophilic droplet is disposed in the second space of the first containing slot. The lipophilic substance is disposed in the first containing slot, wherein the lipophilic substance is immiscible to the active reagent and the hydrophilic droplet, and separates the active reagent from the hydrophilic droplet.

Abstract: A apparatus for microfluid detection for detecting a sample fluid including a plurality of magnetic particles is provided. The apparatus for microfluid detection includes a microfluidic chip and a magnetic generating module. The microfluidic chip includes a substrate and microfluidic channels, wherein the sample fluid is carried by a carry surface of the substrate. The magnetic generating module is adapted for providing a positioning magnetic field and a surrounding magnetic field. The magnetic module controls to move the sample fluid and change a distribution of the magnetic particles in the sample fluid through the positioning magnetic field and the surrounding magnetic field.

Abstract: A method for manipulating a droplet by a droplet manipulating device including a flow channel, a first magnetic field generator, and a second magnetic field generator is provided. The first magnetic field generator includes two first magnetic field modules and are at two sides of the flow channel. The second magnetic field generator is between the two first magnetic field modules and includes multiple second magnetic field coils. The droplet is provided in the flow channel and includes a magnetic particle. A first magnetic field is produced on the flow channel by the first magnetic field modules, so the magnetic particle in the droplet has the direction of magnetic field corresponding to the first magnetic field. A second magnetic field is produced on the flow channel by the second magnetic field coils, for driving the magnetic particle in the droplet to be in motion in the flow channel.

Abstract: The disclosure relates to a droplet manipulating device and a method for manipulating a droplet. The droplets manipulating device includes a first magnetic field generator, a second magnetic field generator, and a flow channel. The first magnetic field generator produces a first magnetic field on the droplet, so that the droplet has the direction of magnetic field corresponding to the first magnetic field. Further, the second magnetic field generator produces a second magnetic field on the droplet so as to drive the droplet to be in motion in the flow channel.

Abstract: A method for manipulating a droplet by a droplet manipulating device including a flow channel, a first magnetic field generator, and a second magnetic field generator is provided. The first magnetic field generator includes two first magnetic field modules and are at two sides of the flow channel. The second magnetic field generator is between the two first magnetic field modules and includes multiple second magnetic field coils. The droplet is provided in the flow channel and includes a magnetic particle. A first magnetic field is produced on the flow channel by the first magnetic field modules, so the magnetic particle in the droplet has the direction of magnetic field corresponding to the first magnetic field. A second magnetic field is produced on the flow channel by the second magnetic field coils, for driving the magnetic particle in the droplet to be in motion in the flow channel.

Abstract: A apparatus for microfluid detection for detecting a sample fluid including a plurality of magnetic particles is provided. The apparatus for microfluid detection includes a microfluidic chip and a magnetic generating module. The microfluidic chip includes a substrate and microfluidic channels, wherein the sample fluid is carried by a carry surface of the substrate. The magnetic generating module is adapted for providing a positioning magnetic field and a surrounding magnetic field. The magnetic module controls to move the sample fluid and change a distribution of the magnetic particles in the sample fluid through the positioning magnetic field and the surrounding magnetic field.

Abstract: The disclosure relates to a droplet manipulating device and a method for manipulating a droplet. The droplets manipulating device includes a first magnetic field generator, a second magnetic field generator, and a flow channel. The first magnetic field generator produces a first magnetic field on the droplet, so that the droplet has the direction of magnetic field corresponding to the first magnetic field. Further, the second magnetic field generator produces a second magnetic field on the droplet so as to drive the droplet to be in motion in the flow channel.

Abstract: A detection chip is provided. The detection chip includes a substrate, an active reagent, a hydrophilic droplet and a lipophilic substance. The substrate includes a first containing slot, wherein the first containing slot includes a first space and a second space adjacent to each other. The active reagent is disposed in the first space of the first containing slot. The hydrophilic droplet is disposed in the second space of the first containing slot. The lipophilic substance is disposed in the first containing slot, wherein the lipophilic substance is immiscible to the active reagent and the hydrophilic droplet, and separates the active reagent from the hydrophilic droplet.

Abstract: A concentration method of dielectrophoretic particles includes: providing a fluid pipe structure, wherein the fluid pipe structure has a protrudent structure lateral protruding inwardly so as to form a line-like gate; making a fluid containing particles to be measured flow through the fluid pipe structure; and applying an electrical field through the line-like gate so as to produce a dielectrophoresis force to concentrate the particles to be measured.

Abstract: A dielectrophoretic particle concentrator includes first substrate, detection electrodes, second substrate, protrudent structure and edge wall structures. The first substrate extends along first direction. The detection electrodes are disposed on the first substrate and extend along second direction. The second direction crosses the first direction. The second substrate is disposed over the first substrate and extends along the first direction. The protrudent structure is disposed on the second substrate and protruded towards the first substrate. A top portion of the protrudent structure includes a line-like structure extending along the second direction and adjacent to the detection electrodes. The edge wall structures are integrated with the first substrate and the second substrate, to form pipe-like structure to enable a fluid flowing through the protrudent structure from an end to another end.

Abstract: A method of manufacturing a three-dimensional nanochannel device is provided. In the method, a first insulation layer is formed on a substrate, a first opening is formed in the first insulation layer, and a patterned photoresist is formed on the first insulation layer. The patterned photoresist includes at least one second opening, wherein the second opening is adjacent to the first opening and exposes the first insulation layer. Afterwards, the first insulation layer is etched and the substrate is also continued to be etched by using the patterned photoresist as a mask, so as to form a housing space, wherein a depth of the housing space is at least two orders greater than a thickness of the first insulation layer. Thereafter, the patterned photoresist is removed, and a second insulation layer is formed on a surface of the substrate.

Abstract: A three-dimensional nanochannel device and a method of manufacturing the same are provided. In the device, a first substrate, a second substrate, and a channel layer sandwiched by the first and the second substrates are included. At least one channel is constituted by the first and the second substrates and the channel layer and includes a fluid inlet, a fluid outlet, and at least one condensed channel between the fluid inlet and the fluid outlet. The condensed channel at least has a first size and a second size on an X-Y plane and has a third size and a fourth size on an X-Z plane. A difference between the first size and the second size is about at least two orders in scale, and a difference between the third size and the fourth size is about at least two orders in scale.

Abstract: A pneumatic micropump is provided. The pneumatic micropump includes a fluidic channel layer, an upper substrate, a lower substrate, an upper membrane and a lower membrane. The fluidic channel includes a fluid inlet a reservoir, and a fluid outlet, wherein the fluid passes through the fluid inlet, the reservoir and the fluid outlet, successively. The upper substrate includes an upper pneumatic chamber facing the reservoir. The lower substrate includes a lower pneumatic chamber facing the reservoir. The upper membrane is disposed between the upper pneumatic chamber and the reservoir, and the lower membrane is disposed between the lower pneumatic chamber and the reservoir.

Abstract: A three-dimensional nanochannel device and a method of manufacturing the same are provided. In the device, a first substrate, a second substrate, and a channel layer sandwiched by the first and the second substrates are included. At least one channel is constituted by the first and the second substrates and the channel layer and includes a fluid inlet, a fluid outlet, and at least one condensed channel between the fluid inlet and the fluid outlet. The condensed channel at least has a first size and a second size on an X-Y plane and has a third size and a fourth size on an X-Z plane. A difference between the first size and the second size is about at least two orders in scale, and a difference between the third size and the fourth size is about at least two orders in scale.

Abstract: A dielectrophoretic particle concentrator includes first substrate, detection electrodes, second substrate, protrudent structure and edge wall structures. The first substrate extends along first direction. The detection electrodes are disposed on the first substrate and extend along second direction. The second direction crosses the first direction. The second substrate is disposed over the first substrate and extends along the first direction. The protrudent structure is disposed on the second substrate and protruded towards the first substrate. A top portion of the protrudent structure includes a line-like structure extending along the second direction and adjacent to the detection electrodes. The edge wall structures are integrated with the first substrate and the second substrate, to form pipe-like structure to enable a fluid flowing through the protrudent structure from an end to another end.