Abstract: A method for establishing a multi-degree-of-freedom bending-shear coupling nonlinear analysis model and a method for applying the model are provided. Collected building information is counted and analyzed; floor mass and floor moment of inertia of the building are calibrated by a mass and moment of inertia matrix; based on different types of the building structures, a proportion of the bending stiffness and a proportion of the shear stiffness of each floor of the building are different; global damping of the building is calibrated by a global damping matrix based on the processed building information; the global elastic rigidness of the building is calibrated by a global elastic rigidness matrix; a weight of the shear stiffness matrix and a weight of the bending stiffness matrix in the global elastic stiffness matrix are controlled; and a multi-degree-of-freedom bending-shear coupling model is established.

Abstract: The present disclosure relates to the field of biotechnology, and in particular to method for modifying a high-yield L-lysine-producing strain engineering and application thereof. The present disclosure provides a recombinant strain obtained by knocking out the cbrB gene and/or the cbrC gene in the genome of E. coli. Fermentation of the recombinant strain can increase the yield and conversion rate of L-lysine and reduce the mortality rate of the strain during the fermentation process. The present disclosure has significant application value. Through experimental studies, it was found that these two genes have a synergistic effect on E. coli. L-lysine hydrochloride imposes osmotic stress resistance on the strains, and silencing the related gene proteins can alter the membrane lipid composition (increasing a proportion of unsaturated fatty acids), maintain membrane fluidity, improve tolerance to lysine hydrochloride, and reduce the mortality rate in the late stage of fermentation.

Abstract: A series-parallel circulating irrigation device for ecological restoration slopes at different elevations in pumped-storage power station and operation method thereof, comprising a pipeline network system for irrigation water delivery; partial devices of the pumped-storage power station are connected to the pipeline network system to provide an irrigation water source; the pipeline network system is laid on the ecological restoration slopes; the pipeline network system is connected to a data acquisition and processing system, and an interior of the pipeline network system is provided with a filtration device. This device can fully utilize pumped-storage water resources, achieve targeted automatic irrigation for ecological restoration slopes at different elevations, and can also filter excess water resources for use in lower slope irrigation and power generation. This device organically integrates with the pumped-storage system, achieving water resource recycling and energy conservation.

Abstract: An all-terrain vehicle has a prime mover assembly which includes a tall and narrow gearbox. The collection of shafts in the gearbox include a (highest) input shaft, a (lowest) front output shaft, and a (forwardmost) reverse shaft, as well as a (rearwardmost) rear output gear. A shaft spacing aspect ratio of the vertical shaft spacing distance to the horizontal shaft spacing distance is greater than or equal to 0.85 and less than or equal to 1.4. A parking pawl control linkage has a pawl control wheel, with a park lobe, rotationally mounted about an axis of the shifting shaft used for gear shifting. Rotation of the shifting shaft into park causes rotation of the pawl control wheel such that the park lobe pushes on the parking pawl to move the parking pawl from the disengaged position to the engaged position.

Abstract: A robotic device for reducing electromagnetic interference (EMI) noise and suppress leakage current is provided. The robotic device comprises a controller configured to control phase-shift switching of a plurality of axes to reduce electromagnetic interference (EMI) noise and suppress leakage current, an electrical circuit, and a plurality of loads coupled to a plurality of motors of the plurality of axes. The electrical circuit comprises a power source and rectifier; an EMI filter configured to further reduce the EMI noise and suppress the leakage current from the plurality of axes; and the plurality of axes comprising a plurality of drives and a plurality of motors that are configured to operate the plurality of loads.

Abstract: The present invention provides a method for efficiently producing L-homophenylalanine and a strain producing L-homophenylalanine. In the present invention, a new route for the synthesis of L-homophenylalanine by a cascade enzymatic method using cheap benzaldehyde and pyruvic acid as raw materials is designed. By constructing the pathway-related enzymes into the same E. coli strain, a recombinant E. coli is obtained, with which L-homophenylalanine is catalytically produced through reaction in a 5 L reactor, with a yield of 100.9 g/L, a conversion rate of 94%, and ee>99%. Compared with the existing main methods for producing L-HPA, the production cost of L-homophenylalanine is greatly reduced. Thus, the present invention has good application prospects.

Abstract: A stirring method includes: stirring cell suspension by circulating the cell suspension in a circulation path including a bioreactor; acquiring a predetermined or selected physical quantity related to the cell suspension at a measurement target site in the circulation path over time; and determining if a uniform diffusion state in which cells are uniformly diffused in the cell suspension is reached by comparing a degree of variation of the physical quantity to the a predetermined or selected degree.

Abstract: A cell transfer method includes supplying a culture medium to a bioreactor using a pipe in communication with the bioreactor, where cells remain in the pipe (first circulation flow path) to transfer cells remaining in the pipe to the bioreactor; acquiring a predetermined physical quantity related to the supply of the culture medium to the bioreactor; and stopping the supply of the culture medium to the bioreactor when the physical quantity reaches a predetermined threshold value.

Abstract: A distribution measurement device includes: a plurality of electrodes provided in a bioreactor, a selection unit that is configured to selects a pair of the electrodes from the plurality of electrodes, and a measurement unit that is configured to measure a physical quantity between the pair of electrodes selected by the selection unit.

Abstract: Provided are modifications of human interleukin-2 (IL-2) and interferon ? (IFN?), a fusion protein formed by modified IL-2, IFN? or both of IL-2/IFN? and an Fc, and design, preparation and use of the fusion protein. By changing the binding capacity of IFN? to a receptor thereof by means of gene mutation, a superior fusion protein is acquired, which may overcome the defects of weak specificity and serious adverse effects of existing drugs.

Abstract: The present invention relates to a recombinant strain for producing shikimic acid, in which a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed The target gene that regulates the asymmetric cell division includes cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production include DAHP synthase coding gene aroG, 3-dehydroquinate synthase coding gene aroB, and transketolase coding gene tktA. The recombinant strain of the present invention realizes the de novo synthesis of shikimic acid using glucose as a substrate, with a low cost. After fermentation with the strain in a 7.5 L fermentor, the highest production of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity of shikimic acid is 1.1 g/L/h.

Abstract: The present invention provides recombinant Escherichia coli for producing glutarate, a construction method and use thereof. A double-plasmid recombinant bacterium is constructed through molecular biological means for co-expressing an aldehyde synthase (AAS) gene, an amine oxidase Mao (gene) and an aldehyde dehydrogenase (Glox) gene. The constructed expression plasmids are introduced into the Escherichia coli to reconstruct to obtain recombinant cells. A recombination strain for efficiently producing glutarate is obtained through amicillin resistance and kanamycin resistance combined plate screening. Efficient production of the glutarate is achieved by optimizing concentration of a substrate, cell concentration and a transformation temperature. L-lysine with a concentration of 30 g/L may be transformed into 19.

Abstract: The present disclosure relates to light guides and near-eye display apparatuses. One example light guide includes a light guide plate and a two-dimensional grating that is disposed on a surface of the light guide plate. The two-dimensional grating includes a plurality of grating units arranged in a planar shape, the grating units are arranged at an interval of a first distance along a first direction and at an interval of a second distance along a second direction, and the first direction intersects with the second direction. The two-dimensional grating includes a light egress area and a light ingress area that are arranged along a third direction, and a fourth direction is perpendicular to the third direction.

Abstract: Disclosed is an acid phosphatase mutant obtained from Pseudomonas aeruginosa having the amino acid sequence of SEQ ID NO:3 and method of using the mutant in the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, the problems of a high substrate cost, environmental pollution and the like are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

Abstract: The present disclosure relates to a medical silicon rubber having high mechanical property and a preparation method thereof. The medical silicon rubber comprises the following raw materials in parts by weight: 50˜90 parts of silicon rubber, 10˜50 parts of polyurethane, 10˜50 parts of fillers, 2˜6 parts of hydrogen-containing silicon oil and 2˜5 parts of a vulcanizing agent. The preparation method comprises: evenly mixing a part of fillers with silicon rubber in an internal mixer, and evenly mixing the other part of fillers with polyurethane in the internal mixer; and mixing the mixed polyurethane on an open type rubber refining machine, then successively adding hydrogen-containing silicon oil, the evenly mixed silicon rubber and the vulcanizing agent into the open type rubber refining machine many times by small portions, and finally vulcanizing the mixed rubber on a plate rubber vulcanizing machine for 5˜15 min at 150˜190° C.

Abstract: A cell culture device includes a cell culture circuit, a sensor that is disposed in the cell culture circuit and is configured to measure turbidity of a cell fluid, and a calibration unit. The calibration unit is configured to calculate, as a first concentration, a cell concentration of a culture medium that includes the cell fluid, to calculate, as a second concentration, a cell concentration of a culture medium that includes no cells, and to create a calibration curve for a measurement value of the sensor and the turbidity of the cell fluid by associating the first concentration with a first measurement value for the culture medium that includes the cell fluid and associating the second concentration with a second measurement value for the culture medium that includes no cells.

Abstract: The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.

Abstract: A robotic device for reducing electromagnetic interference (EMI) noise and suppress leakage current is provided. The robotic device comprises a controller configured to control phase-shift switching of a plurality of axes to reduce electromagnetic interference (EMI) noise and suppress leakage current, an electrical circuit, and a plurality of loads coupled to a plurality of motors of the plurality of axes. The electrical circuit comprises a power source and rectifier; an EMI filter configured to further reduce the EMI noise and suppress the leakage current from the plurality of axes; and the plurality of axes comprising a plurality of drives and a plurality of motors that are configured to operate the plurality of loads.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: The invention provides a method for preparing pyrrolidone, and the invention provides a method for catalytically preparing pyrrolidone with ?-aminobutyric acid in the presence of carnitine-CoA ligase CaiC. The carnitine-CoA ligase CaiC has an amino acid sequence as shown in SEQ ID NO:1. The ligase has catalytic activity in the cyclization of ?-aminobutyric acid to produce pyrrolidone. The carnitine-CoA ligase provided in the present invention affords a yield of pyrrolidone of 3.26 g/L and a molar yield of 39.53% in 24 h when ?-aminobutyric acid is used as a substrate, thus reducing the production period, improving the production of pyrrolidone, and accelerating the industrialization process of producing pyrrolidone by enzymatic conversion method.