Abstract: The present invention provides recombinant Escherichia coli for producing glutarate, a construction method and use thereof. A double-plasmid recombinant bacterium is constructed through molecular biological means for co-expressing an aldehyde synthase (AAS) gene, an amine oxidase Mao (gene) and an aldehyde dehydrogenase (Glox) gene. The constructed expression plasmids are introduced into the Escherichia coli to reconstruct to obtain recombinant cells. A recombination strain for efficiently producing glutarate is obtained through amicillin resistance and kanamycin resistance combined plate screening. Efficient production of the glutarate is achieved by optimizing concentration of a substrate, cell concentration and a transformation temperature. L-lysine with a concentration of 30 g/L may be transformed into 19.

Abstract: The disclosure provides a medical sealant glue capable of promoting wound healing and a preparation method thereof. The medical sealant glue is formed by covalent cross-linking of two components after being physically mixed by a mixing tool, wherein the first component is a nucleophilic component, and the second component is an electrophilic component. The medical sealant glue has a gelation time of 1-10 s, a swelling ratio of 0-100%, a bursting strength of 100-250 mmHg, and a degradation time of 0.5-300 days. The nucleophilic component of the medical sealant glue contains exosome or hyaluronic acid and chitosan, and thus the medical sealant glue not only has the effect of promoting wound healing, but also has antibacterial and anti-infection effects.

Abstract: A self-contained actuator for use in a robot manipulator includes: a housing, in which are arranged in axial sequence a gear, a motor, a brake, a sensor and a motor drive. The motor drive includes wide-bandgap/WBG electronics and is arranged in heat-dissipating relationship with a free end of the housing. Through the motor and some further components, there is provided a central channel for receiving a cable connected to at least one further actuator of the robot manipulator. The channel may be angled or straight. In embodiments where the motor drive is hollow, the central channel extends between the axial ends of the actuator. There is further provided an all-in-one industrial robot comprising a robot manipulator, in which one or more self-contained actuators with the above characteristics are installed and a robot controller is physically integrated.

Abstract: A probe request sent by at least one second router is received. A router to be networked is determined from the at least one second router according to the probe request. A networking request carrying networking matching information is returned to the router to be networked. Wireless access of the first router is enabled. The networking matching information is provided to the router to be networked for networking matching. After the router to be networked has enabled wireless access of the router to be networked based on the networking request, networking is completed by establishing, based on wireless access of the first router and wireless access of the router to be networked, a connection with the router to be networked.

Abstract: The present invention relates to a recombinant strain for producing shikimic acid, in which a target gene that regulates the asymmetric cell division and target genes that regulate the shikimic acid production are expressed The target gene that regulates the asymmetric cell division includes cytoskeletal protein PopZ coding gene popZ, and the target genes that regulate the shikimic acid production include DAHP synthase coding gene aroG, 3-dehydroquinate synthase coding gene aroB, and transketolase coding gene tktA. The recombinant strain of the present invention realizes the de novo synthesis of shikimic acid using glucose as a substrate, with a low cost. After fermentation with the strain in a 7.5 L fermentor, the highest production of shikimic acid is 88.1 g/L, the yield is 0.33 g/g, and the production intensity of shikimic acid is 1.1 g/L/h.

Abstract: A first network node having gained access to a preset network receives a probe message broadcast by a second network node seeking access to the preset network. The probe message carries a second Internet Protocol (IP) address of the second network node. If the probe message includes a configuration mechanism identifier, the first network node unicasts a reply message of the probe message to the second network node based on the second IP address. The reply message carries a first IP address of the first network node. The first IP address is configured for establishing a network communication channel between the second network node and the first network node. The first network node sends network configuration information to the second network node through the network communication channel. The network configuration information is configured to allow the second network node to access the preset network.

Abstract: Disclosed is an aromatic compound. Such compounds can locally produce a long-lasting nerve blocking effect, have an analgesic effect, an anti-pruritic effect, etc., and can be used in the preparation of a long-acting local anaesthetic drug, a local analgesic drug and an anti-pruritic drug.

Abstract: An access method is provided for an access point (AP). The method is applied to a first AP, and includes: responsive to determining that an access restriction rule allows a second AP to access the first AP, interface configuration information of the first AP is determined according to the access restriction rule; and an access interface of the first AP is configured according to the interface configuration information, where the access interface is configured for the second AP to access the first AP in response to determining that the access interface is configured to allow access.

Abstract: A magnet coil for magnetic Czochralski single crystal growth includes: a first coil, a second coil, and an auxiliary coil arranged between the first coil and the second coil. A distance between the first coil and a first edge of the auxiliary coil close to the first coil is equal to a distance between the second coil and a second edge of the auxiliary coil close to the second coil. The auxiliary coil, the first coil and the second coil have a common central axis. When being energized, a direction of a current in the first coil is opposite to a direction of a current in the second coil, and a magnetic field generated by a current in the auxiliary coil is used for enhancing a cusp magnetic field between the first coil and the second coil.

Abstract: A first network node having gained access to a preset network receives a probe message broadcast by a second network node seeking access to the preset network. The probe message carries a second Internet Protocol (IP) address of the second network node. If the probe message includes a configuration mechanism identifier, the first network node unicasts a reply message of the probe message to the second network node based on the second IP address. The reply message carries a first IP address of the first network node. The first IP address is configured for establishing a network communication channel between the second network node and the first network node. The first network node sends network configuration information to the second network node through the network communication channel. The network configuration information is configured to allow the second network node to access the preset network.

Abstract: An access method is provided for an access point (AP). The method is applied to a first AP, and includes: responsive to determining that an access restriction rule allows a second AP to access the first AP, interface configuration information of the first AP is determined according to the access restriction rule; and an access interface of the first AP is configured according to the interface configuration information, where the access interface is configured for the second AP to access the first AP in response to determining that the access interface is configured to allow access.

Abstract: According to one aspect of the present disclosure, a single-stage converter includes a rectifying circuit and a buck-boost circuit. The buck-boost circuit includes an inductor with a center tap configured to supply an output of the buck-boost circuit to the rectifying circuit. The buck-boost circuit also includes first and second interleaved arms arranged in parallel with a voltage input of the single-stage converter. The first and second interleaved arms are each coupled to the inductor and include a plurality of switches operable to control the output of the buck-boost circuit.

Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: A welding-rolling integrated composite forming method uses the low heat input wire filling welding method to cooperate with the optimization of the welding process, and at the same time the rolling device is used to roll the weld reinforcement area of the welded joint, realizing the precise control of the structure and properties of each area of the welded joint by using the plastic flow and deformation strengthening effect of the weld reinforcement of the welded joint during rolling process. The integrated composite forming device adjusts the distances between the welding mechanism, the rolling mechanism and the metal sheet to-be-welded and rolled by the coordination of each mechanism, and carries out synchronous rolling on the weld reinforcement of the welded joint.

Abstract: A probe request sent by at least one second router is received. A router to be networked is determined from the at least one second router according to the probe request. A networking request carrying networking matching information is returned to the router to be networked. Wireless access of the first router is enabled. The networking matching information is provided to the router to be networked for networking matching. After the router to be networked has enabled wireless access of the router to be networked based on the networking request, networking is completed by establishing, based on wireless access of the first router and wireless access of the router to be networked, a connection with the router to be networked.

Abstract: Disclosed is a mutant of acid phosphatase and an application thereof, belonging to the technical field of biological engineering. The disclosure provides a mutant of acid phosphatase PaAPaseMu3. By expressing the mutant of acid phosphatase PaAPaseMu3 in Escherichia coli and using a whole-cell conversion method, L-ascorbic acid is transformed into L-ascorbate-2-phosphate. Moreover, a catalytic system of the mutant of acid phosphatase PaAPaseMu3 is optimized from the aspects of reaction pH and temperature, so that a yield of L-ascorbate-2-phosphate can reach 90.1 g/L and a molar yield can reach 75.1%. Therefore, the problems of a high substrate cost, environmental pollution and the like before are greatly reduced, laying a foundation for the industrial green production of L-ascorbate-2-phosphate.

Abstract: Unique systems, methods, techniques and apparatuses of photovoltaic (PV) string power systems are disclosed. One exemplary embodiment is a PV power system comprising a plurality of PV strings and a floating DC-DC optimizer. The floating DC-DC optimizer comprises a first DC bus rail, a second DC bus rail, a plurality of input legs, each leg being coupled across the first DC bus rail and second DC bus rail, including two input leg semiconductor switches coupled at an input terminal, each input terminal being structured to receive an input current from an end of one PV string of the plurality of PV strings, and an output leg including two output leg semiconductor devices coupled at an output terminal.

Abstract: The disclosure discloses construction and application of an engineered strain of E. coli for producing malic acid by fixing CO2, and belongs to the field of fermentation. The engineered strain is obtained by performing genetic engineering transformation on Escherichia coli MG1655; the genetic engineering transformation includes knocking out a fumarate reductase gene, a fumarase gene, a lactate dehydrogenase gene and an alcohol dehydrogenase gene and freely overexpressing a formate dehydrogenase, an acetyl coenzyme A synthetase, an acylated acetaldehyde dehydrogenase, a formaldehyde lyase, a dihydroxyacetone kinase, a malic enzyme and a phosphite oxidoreductase to obtain a strain GH0407. The strain is used for producing malic acid by fermentation, anaerobic fermentation is performed for 72 hours with CO2 and glucose as a co-substrate, the production of malic acid reaches 39 g/L, the yield is 1.53 mol/mol, and accumulation of malic acid in the original strain is not achieved.

Abstract: Systems, methods, techniques and apparatuses of power switch protection are disclosed. One exemplary embodiment is a protection system for a power switch comprising a resistor and a detection circuit. The resistor is coupled in series with a decoupling capacitor. The detection circuit is structured to receive a voltage of the resistor, determine a short circuit is occurring based on the received electrical characteristic, and transmit a fault trigger signal in response to determining the short circuit is occurring.

Abstract: The present disclosure relates to a medical silicon rubber having high mechanical property and a preparation method thereof. The medical silicon rubber comprises the following raw materials in parts by weight: 50˜90 parts of silicon rubber, 10˜50 parts of polyurethane, 10˜50 parts of fillers, 2˜6 parts of hydrogen-containing silicon oil and 2˜5 parts of a vulcanizing agent. The preparation method comprises: evenly mixing a part of fillers with silicon rubber in an internal mixer for later use, and evenly mixing the other part of fillers with polyurethane in the internal mixer; and mixing the mixed polyurethane on an open type rubber refining machine, then successively adding hydrogen-containing silicon oil, the evenly mixed silicon rubber and the vulcanizing agent into the open type rubber refining machine many times by small portions, and finally vulcanizing the mixed rubber on a plate rubber vulcanizing machine for 5˜15 min at 150˜190° C.