Abstract: A focused treatment tip (FTT) for controlling the evaporation rate and providing targeted delivery of low temperature liquified gases for contact with living tissue includes a contoured body. When filled with liquified gas, the device insulates the gas from waste heat sources, such as the surrounding environment. The device can control the evaporation rate of the liquified gas at the treatment site. The controlled evaporation rate affects the rate of heat transfer from the treated tissue allowing for controlled exposure times and desired outcomes. The device can be used with various application tips to further define the target tissue area to be treated while minimizing collateral damage to surrounding tissue and isolating the gas within the contoured body and focusing heat transfer to the desired treatment area. The device may use transparent materials that make the treatment visible to the operator while the liquified gas is evaporating.

Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section.

Abstract: A focused treatment tip (FTT) for controlling the evaporation rate and providing targeted delivery of low temperature liquified gases for contact with living tissue includes a contoured body. When filled with liquified gas, the device insulates the gas from waste heat sources, such as the surrounding environment. The device can control the evaporation rate of the liquified gas at the treatment site. The controlled evaporation rate affects the rate of heat transfer from the treated tissue allowing for controlled exposure times and desired outcomes. The device can be used with various application tips to further define the target tissue area to be treated while minimizing collateral damage to surrounding tissue and isolating the gas within the contoured body and focusing heat transfer to the desired treatment area. The device may use transparent materials that make the treatment visible to the operator while the liquified gas is evaporating.

Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section.

Abstract: A flow modulation device 300 for controlling a rheological state of a dispensed pressurized fluid includes a porous element 304 and an exit tube. The porous element 304 is in fluid communication with a distal end of an outlet tube 303 and receives pressurized fluid in a first rheological state. The porous element 304 includes a plurality of channels that divide a flow channel into a plurality of flow paths through which the pressurized fluid flows and that modulates the flow of the pressurized fluid. The exit tube 305 includes proximal end 355 and distal end 345 and an intermediate body including a sidewall 365 defining a hollow internal lumen 375. The exit tube 305 is in fluid communication with the porous element 304 and receives the modulated pressurized fluid from the plurality of flow paths and refocuses the fluid to dispense the pressurized fluid in a second rheological state.

Abstract: The portable cryosurgical device is economical, easy to use and operate, and delivers cryogenic material such as nitrous oxide and carbon dioxide gas in any direction or orientation. The delivered materials destroy target tissue using extremely cold temperatures and the abrasives formed when the cryogenic material becomes a solid. The device includes a portable countertop enclosure housing a cryogen source, a cryogen flow tube in fluid communication with the cryogen source, and a flow path assembly. The flow path assembly includes a valve between the cryogen source and the cryogen flow tube having substantially zero dead volume. The flow path assembly can include a quick-connect cryogen tank adapter and an in-line cryogen filter. The flow path assembly delivers liquefied compressed gas from the cryogen source to a terminal end of the cryogen flow tube without a phase change occurring in the flow path.

Abstract: A flow modulation device 300 for controlling a rheological state of a dispensed pressurized fluid includes a porous element 304 and an exit tube. The porous element 304 is in fluid communication with a distal end of an outlet tube 303 and receives pressurized fluid in a first rheological state. The porous element 304 includes a plurality of channels that divide a flow channel into a plurality of flow paths through which the pressurized fluid flows and that modulates the flow of the pressurized fluid. The exit tube 305 includes proximal end 355 and distal end 345 and an intermediate body including a sidewall 365 defining a hollow internal lumen 375. The exit tube 305 is in fluid communication with the porous element 304 and receives the modulated pressurized fluid from the plurality of flow paths and refocuses the fluid to dispense the pressurized fluid in a second rheological state.

Abstract: Systems, devices, and methods for delivery of a topical substance, including medicaments and/or cosmetic formulas, with carbon dioxide to the surface of the skin. The systems regulate the pH, temperature and viscosity of the topical substance, which provides benefits to the treatment of the skin. For beauty treatments, the systems lessen wrinkles of the skin and hydrate at the same time. For medicaments with active ingredients, the systems enhance delivery and ionic state using pH adjustment. The systems include devices that contain a reservoir of carbon dioxide, which flows through a heat control path where the temperature of the carbon dioxide is adjusted to a desired setting. The temperature-controlled carbon dioxide then mixes with the topical substance in a chamber, which is pressed against or applied to the skin to achieve the benefits.

Abstract: The portable cryosurgical device is economical, easy to use and operate, and delivers cryogenic material such as nitrous oxide and carbon dioxide gas in any direction or orientation. The delivered materials destroy target tissue using extremely cold temperatures and the abrasives formed when the cryogenic material becomes a solid. The device includes a portable countertop enclosure housing a cryogen source, a cryogen flow tube in fluid communication with the ciyogen source, and a flow path assembly. The flow path assembly includes a valve between the ciyogen source and the cryogen flow tube having substantially zero dead volume. The flow path assembly can include a quick-connect cryogen tank adapter and an in-line ciyogen filter. The flow path assembly delivers liquefied compressed gas from the cryogen source to a terminal end of the cryogen flow tube without a phase change occurring in the flow path.

Abstract: A focused treatment tip (FTT) for controlling the evaporation rate and providing targeted delivery of low temperature liquified gases for contact with living tissue includes a contoured body. When filled with liquified gas, the device insulates the gas from waste heat sources, such as the surrounding environment. The device can control the evaporation rate of the liquified gas at the treatment site. The controlled evaporation rate affects the rate of heat transfer from the treated tissue allowing for controlled exposure times and desired outcomes. The device can be used with various application tips to further define the target tissue area to be treated while minimizing collateral damage to surrounding tissue and isolating the gas within the contoured body and focusing heat transfer to the desired treatment area. The device may use transparent materials that make the treatment visible to the operator while the liquified gas is evaporating.

Abstract: A flow modulation device 300 for controlling a rheological state of a dispensed pressurized fluid includes a porous element 304 and an exit tube. The porous element 304 is in fluid communication with a distal end of an outlet tube 303 and receives pressurized fluid in a first rheological state. The porous element 304 includes a plurality of channels that divide a flow channel into a plurality of flow paths through which the pressurized fluid flows and that modulates the flow of the pressurized fluid. The exit tube 305 includes proximal end 355 and distal end 345 and an intermediate body including a sidewall 365 defining a hollow internal lumen 375. The exit tube 305 is in fluid communication with the porous element 304 and receives the modulated pressurized fluid from the plurality of flow paths and refocuses the fluid to dispense the pressurized fluid in a second rheological state.

Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section.

Abstract: A channel-less microfluidic pump includes a cartridge including a substrate and an actuatable film layer disposed on the substrate, and a manifold having at least three actuatable void volumes separated by a plurality of wall sections and an actuatable flexible layer disposed on the manifold interfacing the actuatable film layer. In operation, the pump can be in an unactuated state wherein the actuatable film layer is disposed against the surface of the substrate or an actuated state wherein at least a portion of the flexible layer and a corresponding portion of the actuatable film layer are deflected into a corresponding void volume thus forming a fluidic volume between the deflected portion of the actuatable film layer and the surface of the substrate. In the actuated state, there is a fluidic gap between immediately adjacent void volumes formed by a thinned region of the flexible layer at a point of contact with a top surface of a wall section.

Abstract: Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

Abstract: Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

Abstract: Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

Abstract: A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing.