Patents by Inventor Lishan Zhao
Lishan Zhao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8546118Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: March 7, 2007Date of Patent: October 1, 2013Assignee: Verenium CorporationInventors: David P. Weiner, Lishan Zhao, Peter Luginbuhl, Ellen Burke, Toby Richardson, Paula M. Hicks
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Patent number: 8435765Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: May 20, 2008Date of Patent: May 7, 2013Assignee: Cargill, IncorporatedInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, James R. Millis, John Rosazza, David P. Weiner, Lishan Zhao
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Patent number: 8372989Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: November 3, 2004Date of Patent: February 12, 2013Assignee: Cargill, IncorporatedInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, John Rosazza, Lishan Zhao, David P. Weiner
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Publication number: 20120270260Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: ApplicationFiled: July 5, 2012Publication date: October 25, 2012Applicant: AMYRIS, INC.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main
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Publication number: 20120266328Abstract: The invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, ?-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.Type: ApplicationFiled: January 20, 2012Publication date: October 18, 2012Applicant: BP CORPORATION NORTH AMERICA INC.Inventors: KEVIN A. GRAY, LISHAN ZHAO, MICHELLE H. CAYOUETTE
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Patent number: 8236512Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: GrantFiled: February 1, 2012Date of Patent: August 7, 2012Assignee: Amyris, Inc.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main
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Publication number: 20120196315Abstract: The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.Type: ApplicationFiled: February 1, 2012Publication date: August 2, 2012Applicant: AMYRIS, INC.Inventors: Lishan Zhao, Lan Xu, Patrick Westfall, Andrew Main
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Patent number: 8101393Abstract: The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a ?-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, ?-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass.Type: GrantFiled: December 8, 2006Date of Patent: January 24, 2012Assignee: BP Corporation North America Inc.Inventors: Kevin A. Gray, Lishan Zhao, Michelle H. Cayouette
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Publication number: 20120010104Abstract: The invention provides growth selection methods for screening an environmental or a mixed population library of nucleic acids comprising uncharacterized nucleic acids for the presence of a nucleic acid encoding an enzyme of interest. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzymes of interest, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases, are also provided.Type: ApplicationFiled: June 21, 2011Publication date: January 12, 2012Inventors: David Weiner, Tim Hitchman, Lishan Zhao, Mark J. Burk, Grace Desantis, Sarah Richardson Hanson, Aileen Milan, Toby Richardson, Patti Kretz, William Greenberg
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Publication number: 20120009634Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: September 16, 2011Publication date: January 12, 2012Inventors: Ellen BURKE, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Patent number: 8043837Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: GrantFiled: March 7, 2007Date of Patent: October 25, 2011Assignee: Cargill, IncorporatedInventors: Ellen Burke, Steven John Gort, Paula M. Hicks, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Patent number: 7981844Abstract: The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. This invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided.Type: GrantFiled: June 21, 2002Date of Patent: July 19, 2011Assignee: Verenium CorporationInventors: David Weiner, Tim Hitchman, Lishan Zhao, Mark J. Burk, Grace Desantis, Sarah Richardson Hanson, Aileen Milan, Toby Richardson, Patti Kretz, William Greenberg
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Publication number: 20100095390Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: March 7, 2007Publication date: April 15, 2010Inventors: David P. Weiner, Lishan Zhao, Peter Luginbuhl, Ellen Burke, Toby H. Richardson, Paula M. Hicks
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Publication number: 20090220480Abstract: The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a ?-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, ?-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass.Type: ApplicationFiled: December 8, 2006Publication date: September 3, 2009Applicant: VERENIUM CORPORATIONInventors: Kevin A. Gray, Lishan Zhao, Michelle H. Cayouette
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Patent number: 7582455Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.Type: GrantFiled: April 26, 2006Date of Patent: September 1, 2009Assignee: Cargill, IncorporatedInventors: Brian J. Brazeau, Ellen Burke, Mervyn DeSouza, Steven J. Gort, Paula M. Hicks, Sherry R. Kollmann, Peter Luginbuhl, Sara C. McFarlan, Toby Richardson, Fernando A. Sanchez-Riera, Christopher Solheid, David Weiner, Lishan Zhao
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Patent number: 7572607Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: April 26, 2005Date of Patent: August 11, 2009Assignee: Cargill, IncorporatedInventors: Paula M. Hicks, Sara C. McFarlan, Timothy W. Abraham, Douglas C. Cameron, James R. Millis, John Rosazza, Lishan Zhao, David Paul Weiner
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Publication number: 20090130285Abstract: The present invention relates to novel beverage compositions comprising monatin and methods for making such compositions. The present invention also relates to beverage compositions comprising specific monatin stereoisomers, specific blends of monatin stereoisomers, and/or monatin produced via a biosynthetic pathway in vivo (e.g., inside cells) or in vitro.Type: ApplicationFiled: November 14, 2008Publication date: May 21, 2009Inventors: Timothy W. Abraham, Douglas C. Cameron, Melanie J. Goulson, Paula M. Hicks, Michael G. Lindley, Sara C. McFarlan, James R. Millis, John Rosazza, Lishan Zhao, David P. Weiner
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Publication number: 20090117625Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: ApplicationFiled: May 20, 2008Publication date: May 7, 2009Applicant: Cargill, IncorporatedInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, Jim Millis, John Rosazza, Lishan Zhao, David P. Weiner
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Publication number: 20080020435Abstract: This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: March 7, 2007Publication date: January 24, 2008Applicant: Cargill, IncorporatedInventors: Ellen Burke, Steven Gort, Paula Hicks, Peter Luginbuhl, Sara McFarlan, Toby Richardson, Christopher Solheid, David Weiner, Lishan Zhao
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Publication number: 20060252135Abstract: Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.Type: ApplicationFiled: April 26, 2006Publication date: November 9, 2006Applicant: Cargill, IncorporatedInventors: Brian Brazeau, Ellen Burke, Mervyn DeSouza, Steven Gort, Paula Hicks, Sherry Kollmann, Peter Luginbuhl, Sara McFarlan, Toby Richardson, Fernando Sanchez-Riera, Christopher Solheid, David Weiner, Lishan Zhao