Abstract: The invention discloses a method for improving the yield of Bacillus subtilis acetylglucosamine, which belongs to the technical field of genetic engineering. In the invention, the recombinant Bacillus subtilis S5 (S5-PxylA-glmS-P43-GNA1) is taken as a starting strain, and the glmS ribozyme is integrated into the mid of rbs and the promoter sequence of the glmM and pfkA gene, respectively. The ribozyme mutant has the advantage of prolonging the stability of the mRNA and integrated into the mid of rbs and the promoter sequence of the pgi gene. The yield of GlcNAc of the recombinant strain reaches 11.79-20.05 g/L. This laid the foundation for the further metabolic engineering of Bacillus subtilis to produce GlcNAc.

Abstract: The present invention relates to a method for promoting acetylglucosamine synthesis of Bacillus subtilis, which belongs to the field of genetic engineering. The present invention adopts the recombinant Bacillus subtilis BSGNKAP2 as a starting strain, exogenously introducing pyruvate carboxylase BalpycA derived from Bacillus cereus, eliminating the central carbon metabolism overflow of the Bacillus subtilis and avoiding the synthesis of the by-product acetoin; further, five exogenous reducing force metabolic reactions are introduced to replace the reaction of generating NADH in glycolysis pathway and tricarboxylic acid cycle to reconstruct intracellular reducing force metabolism, which specifically comprise glyceraldehyde-3-phosphate ferredoxin dehydrogenase, isocitrate NAD+ dehydrogenase, a malate quinone dehydrogenase, a ketoacid ferredoxin oxidoreductase and a nitrogenase ferritin. In a shake-flask fermentation process using a complex medium, acetylglucosamine yield of the recombinant strain BSGNKAP8 is 24.

Abstract: The present invention discloses a method for producing N-acetylglucosamine (GlcNAc) by co-utilizing glucose and xylose based on CRISPRi, and belongs to the field of genetic engineering. According to the method, Bacillus subtilis BSGNY-Pveg-glmS-P43-GNA1 is used as an original strain, dCas9 induced by xylose and three sgRNA expression fragments targeting to genes zwf, pfkA and glmM respectively are integrated on the genome, and the strain is fermented in a shake flask, so that the titer of GlcNAc reaches 20.5 g/L, the yield of GlcNAc is 0.612 g/g glucose, at the same time, the efficient co-utilizing of glucose and xylose by the recombinant B.s subtilis is achieved, and the foundation for further metabolic engineering transformation of the B. Subtilis to produce GlcNAc and industrialization thereof is laid.

Abstract: A port listening request dynamically generated by an application process hosted in a container can be identified. Whether the application process hosted in the container is trusted can be determined. Responsive to determining that the application process hosted in the container is trusted, a first port to be used as an external port for the application process can be dynamically selected, and a port assignment can be communicated to a container engine, the port assignment indicating the first port is assigned to the application process. The first port can be mapped to a second port assigned as an internal port for the application process. The first port can be opened for the application process.

Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.

Abstract: The invention discloses a method for improving the yield of Bacillus subtilis acetylglucosamine, which belongs to the technical field of genetic engineering. In the invention, the recombinant Bacillus subtilis S5 (S5-PxylA-glmS-P43-GNA1) is taken as a starting strain, and the glmS ribozyme is integrated into the mid of rbs and the promoter sequence of the glmM and pfkA gene, respectively. The ribozyme mutant has the advantage of prolonging the stability of the mRNA and integrated into the mid of rbs and the promoter sequence of the pgi gene. The yield of GlcNAc of the recombinant strain reaches 11.79-20.05 g/L. This laid the foundation for the further metabolic engineering of Bacillus subtilis to produce GlcNAc.

Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.

Abstract: The present invention relates to a human body model acquisition method and a virtual network fitting system based on depth cameras. The method includes the following steps: step S1: generating mark points covering a model body surface and used for determining features of the model body surface on the model body surface, meanwhile collecting depth images of the model body from a plurality of angles by using the depth cameras to acquire a depth image sequence that covers the model body surface and contains the mark points; step S2: carrying target depth information point cloud grid reconstruction on frames of depth images in the depth image sequence; and step S3: mosaicing the reconstructed frames of depth images into a three-dimensional model of the model body according to the mark points in the reconstructed frames of depth images.

Abstract: The present invention provides a recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. The recombinant Bacillus subtilis is obtained by deletion of glmS ribozyme of Bacillus subtilis for regulating expression of glucosamine synthase, and insertion of a terminator and a constitutive promoter. The method comprises constructing a deleting cassette of a glmS ribozyme encoding gene, which includes an upstream homologous fragment, a resistance gene, a terminator sequence, a constitutive promoter sequence and a downstream homologous fragment in sequence; and transforming the deleting cassette into Bacillus subtilis, to obtain the recombinant Bacillus subtilis. In the invention, glucosamine synthase gene (glmS) ribozyme is deleted by homologous recombination, and in host cells, GlcN6P feedback inhibition of expression of glucosamine synthase gene glmS is blocked, and the accumulation of acetylglucosamine is improved.

Abstract: The present invention discloses a television virtual touch control method and system. The television virtual touch control method is used in a television having a depth camera and includes the following steps: acquiring a human body image having depth information by the depth camera in real time; presetting an area excluding a hand area in a human body area as a reference area; extracting the depth information of the reference area and the hand area from the human body image; defining a virtual touch surface between the depth camera and the reference area at a first predetermined distance D1 from the reference area; and determining whether the hand area touches or penetrates through the virtual touch surface according to the depth information of the hand area, the depth information of the reference area and the first predetermined distance D1. The present invention has the beneficial effects of improving touch sensitivity and enhancing user experience.

Abstract: The invention discloses a method for increasing citrate production from genome reconstructed Aspergillus niger. The method is to insert a gene of low affinity glucose transporter, LGT1, to genome of A. niger. The expression level of LGT1 is under control of promoter Pgas. The genome reconstructed A. niger is tolerant to higher fermentation temperature and lower pH than that of the parental strain. Moreover, the production, yield and purity of product from reconstructed A. niger are higher than that of parental strain, and the fermentation time is shorter.

Abstract: The invention discloses a method for improving citric acid production by Aspergillus niger fermentation, which integrates Aspergillus niger GABA pathway succinate semialdehyde dehydrogenase SSD gene into Aspergillus niger genome to obtain recombinant Aspergillus niger strain, and uses recombinant black The Aspergillus strain ferments to produce citric acid; the expression of the succinate semialdehyde dehydrogenase SSD gene is regulated by the Pgas promoter. The method of the invention realizes the expression of succinate semialdehyde dehydrogenase SSD in Aspergillus niger to enhance the GABA pathway so as to strengthen the TCA cycle and promote the synthesis of citric acid.

Abstract: The present disclosure relates to a dedicated ASIC chip system for optical three-dimensional sensing, including a DEPTH ENGINE module, a REGISTER PROCESSOR module, a controller module, a register module, an RGB CMOS driving module, an IR CMOS driving module, an AXI bus interface module, an APB bus interface module, an AXI/APB bridge module, a Flash storage driving module and a DDR3 storage driving module; and when non-optical three-dimensional data is processed, the control module sends an instruction to connect an external Flash memory for processing the non-optical three-dimensional data. When optical three-dimensional data is processed, the control module sends an instruction to simultaneously connect the external Flash memory and an external DDR3 memory for processing the optical three-dimensional data, so as to quickly process high-precision optical three-dimensional data, the resolution of an optical three-dimensional depth image obtained from the processing is high, and the delay is short.

Abstract: The present invention provides a recombinant Bacillus subtilis for producing acetylglucosamine and construction method thereof. The recombinant Bacillus subtilis is obtained by deletion of glmS ribozyme of Bacillus subtilis for regulating expression of glucosamine synthase, and insertion of a terminator and a constitutive promoter. The method comprises constructing a deleting cassette of a glmS ribozyme encoding gene, which includes an upstream homologous fragment, a resistance gene, a terminator sequence, a constitutive promoter sequence and a downstream homologous fragment in sequence; and transforming the deleting cassette into Bacillus subtilis, to obtain the recombinant Bacillus subtilis. In the invention, glucosamine synthase gene (glmS) ribozyme is deleted by homologous recombination, and in host cells, GlcN6P feedback inhibition of expression of glucosamine synthase gene glmS is blocked, and the accumulation of acetylglucosamine is improved.

Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.

Abstract: A case and an electronic device having the case are provided according to the present application. A fan is arranged on a case wall of the case, an air inlet and an air outlet in communication with the air inlet are arranged in the case wall. The fan is arranged inside the case and is located between the air inlet and the air outlet. Each of the fan is mounted in a bottom-to-top manner with its air inlet facing downwards and its air outlet facing upwards. The air inlet is located at a lower end of the case, the air outlet is located at an upper end of the case, and an air duct from bottom to top is formed by the air inlet, the fan and the air outlet.

Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.

Abstract: The present invention provides a method for enhancing N-acetylglucosamine production by usage of a recombinant Bacillus subtilis with a glcK knockout. This invention enhanced the production of GlcNAc by knocking out the glcK gene which encodes a glucokinase, thus eliminating the GlcNAc phosphorylation to GlcNAc-6-P. The specific growth rate and content of GlcNAc in the supernatant of the recombinant Bacillus subtilis with the glcK knockout were 0.15 h?1 and 3.0 g/L, respectively, which were 2.32 times and 2.14 times of those of the control strain without glcK knockout. The recombinant Bacillus subtilis of the present invention would be potentially useful for industrial production of GlcNAc.

Abstract: A display control method for an application program interface is provided. The method includes: determining an appropriate resolution corresponding to the application program to be executed from at least two selectable resolutions; determining configuration information according to the appropriate resolution; establishing a display window having a size equal to a size corresponding to the appropriate resolution according to the configuration information, and loading a resource file corresponding to the application program interface; and rendering the application program interface in the display window on the a display device according to the appropriate resolution and the resource file. Through the above method, the present invention is capable of selecting an appropriate resolution for displaying an application program interface.

Abstract: The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineered B. subtilis Deletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineered B. subtilis for GlcNAc production, which can be further applied to industrial production of GlcNAc.