Patents by Inventor Louis Leong
Louis Leong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20130316350Abstract: The present invention relates to the fields of biotechnology and molecular biology. In particular, the present invention relates to the construction and use of nucleic acid molecules comprising cloning sites which differ in nucleotide sequence. In particular embodiments, the present invention relates to nucleic acid molecules which contain recombination sites with different primer binding sites. These different primer binding sites may be used to sequence different ends of nucleic acid segments located between the two recombination sites.Type: ApplicationFiled: September 14, 2012Publication date: November 28, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon CHESNUT, Louis Leong
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Publication number: 20130236899Abstract: Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.Type: ApplicationFiled: March 7, 2013Publication date: September 12, 2013Applicant: Invisible Sentinel, Inc.Inventors: Nicholas Siciliano, Louis Leong, Martin Patrick Keough, Ashley Shaniece Brown
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Publication number: 20130004996Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: ApplicationFiled: August 26, 2011Publication date: January 3, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan D. CHESNUT, John Carrino, Louis Leong, Knut Madden, Martin A. G. Gleeson, James Fan, Michael A. Brasch, David Cheo, James L. Hartley, Devon R.N. Byrd, Gary F. Temple
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Patent number: 8338091Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.Type: GrantFiled: February 21, 2012Date of Patent: December 25, 2012Assignee: Life Technologies CorporationInventors: Jonathan Chesnut, Miroslav Dudas, Adam Harris, Louis Leong, Knut Madden
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Analyte Detection Devices, Multiplex and Tabletop Devices for Detection of Analyte, and Uses Thereof
Publication number: 20120301893Abstract: Devices and methods for the detection of analytes are disclosed. Devices and methods for detecting food-borne pathogens are disclosed.Type: ApplicationFiled: January 27, 2012Publication date: November 29, 2012Applicant: INVISIBLE SENTINEL, INC.Inventors: Nicholas A. Siciliano, Martin Joseph Bouliane, Louis Leong -
Patent number: 8304189Abstract: The present invention relates to the fields of biotechnology and molecular biology. In particular, the present invention relates to the construction and use of nucleic acid molecules comprising cloning sites which differ in nucleotide sequence. In particular embodiments, the present invention relates to nucleic acid molecules which contain recombination sites with different primer binding sites. These different primer binding sites may be used to sequence different ends of nucleic acid segments located between the two recombination sites.Type: GrantFiled: December 1, 2004Date of Patent: November 6, 2012Assignee: Life Technologies CorporationInventors: Jon Chesnut, Louis Leong
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Publication number: 20120149069Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.Type: ApplicationFiled: February 21, 2012Publication date: June 14, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jonathan D. Chesnut, Knut R. Madden, Miroslav Dudas, Louis Leong, Adam N. Harris
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Publication number: 20120077211Abstract: Compositions, assays, methods, and kits are disclosed for use in applications that utilize oxidation of a chromogenic electron donor such as diaminobenzidine (DAB) to generate a signal. Applications include, but are not limited to, immunohistochemistry, chromogenic in situ hybridization, Western blots, Northern blots, Southern blots, ELISA assays, and microarray detection. The compositions, assays, methods, and kits disclosed herein make use of a novel, stabilized formulation of DAB and a novel, stabilized formulation of hydrogen peroxide.Type: ApplicationFiled: December 15, 2009Publication date: March 29, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Lawrence Greenfield, Shawn Starkenburg, Matthew Shallice, Julie Nyhus, Louis Leong
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Publication number: 20110269120Abstract: The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be assayed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptide having a detectable activity.Type: ApplicationFiled: March 21, 2011Publication date: November 3, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Harry Yim, James Fan, Jonathan Chesnut, Kenneth Frimpong, Laura Vozza-Brown, Louis Leong, Peter Welch, Robert Bennett
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Patent number: 8030066Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: GrantFiled: December 18, 2006Date of Patent: October 4, 2011Assignee: Life Technologies CorporationInventors: Jonathan D. Chesnut, John Carrino, Louis Leong, Knut Madden, Martin Gleeson, James Fan, Michael Brasch, David Cheo, James L. Hartley, Devon R. Byrd, Gray F. Temple
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Publication number: 20110046201Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.Type: ApplicationFiled: April 30, 2008Publication date: February 24, 2011Applicant: INVITROGEN CORPORATIONInventors: Jonathan D. Chesnut, Knut R. Madden, Miroslav Dudas, Louis Leong, Adam N. Harris
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Publication number: 20100009342Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.Type: ApplicationFiled: July 10, 2009Publication date: January 14, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: John Matthew MAURO, Thomas Harry STEINBERG, Lawrence I. GREENFIELD, Louis LEONG
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Publication number: 20090176975Abstract: The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be assayed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptide having a detectable activity.Type: ApplicationFiled: January 25, 2008Publication date: July 9, 2009Applicant: INVITROGEN CORPORATIONInventors: Harry Yim, James Fan, Jonathan Chesnut, Kenneth Frimpong, Laura Vozza-Brown, Louis Leong, Peter Welch, Robert Bennett
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Publication number: 20080241889Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: ApplicationFiled: December 18, 2006Publication date: October 2, 2008Applicant: INVITROGEN CORPORATIONInventors: Jonathan D. Chesnut, John Carrino, Louis Leong, Knut Madden, Martin A.G. Gleeson, James Fan, Michael A. Brasch, David Cheo, James L. Hartley, Devon R.N. Byrd, Gray F. Temple
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Publication number: 20080176213Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.Type: ApplicationFiled: March 27, 2008Publication date: July 24, 2008Applicant: INVITROGEN CORPORATIONInventors: John Matthew Mauro, Thomas Harry Steinberg, Lawrence I. Greenfield, Louis Leong
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Publication number: 20070196838Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions vectors having multiple recombination sites and/or multiple topoisomerase recognition sities. The methods premit the simultaeous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: ApplicationFiled: December 19, 2006Publication date: August 23, 2007Applicant: INVITROGEN CORPORATIONInventors: Jonathan Chesnut, John Carrino, Louis Leong, Knut Madden, Martin Gleeson, James Fan, Michael Brasch, David Cheo, James Hartley, Devon Byrd, Gray Temple
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Patent number: 7244560Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: GrantFiled: December 7, 2001Date of Patent: July 17, 2007Assignee: Invitrogen CorporationInventors: Jonathan D. Chestnut, John Carrino, Louis Leong, Knut Madden, Martin Gleeson, James Fan, Michael A. Brasch, David Cheo, James L. Hartley, Devon R.N. Byrd, Gary F. Temple
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Publication number: 20070134685Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.Type: ApplicationFiled: September 6, 2006Publication date: June 14, 2007Inventors: John MAURO, Thomas STEINBERG, Lawrence GREENFIELD, Louis LEONG
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Patent number: 7198924Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.Type: GrantFiled: March 4, 2004Date of Patent: April 3, 2007Assignee: Invitrogen CorporationInventors: Jonathan D. Chesnut, John Carrino, Louis Leong, Knut Madden, Martin A. G. Gleeson, James Fan, Michael A. Brasch, David Cheo, James L. Hartley, Devon R. N. Byrd, Gary F. Temple
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Publication number: 20050208530Abstract: The present invention relates to the fields of biotechnology and molecular biology. In particular, the present invention relates to the construction and use of nucleic acid molecules comprising cloning sites which differ in nucleotide sequence. In particular embodiments, the present invention relates to nucleic acid molecules which contain recombination sites with different primer binding sites. These different primer binding sites may be used to sequence different ends of nucleic acid segments located between the two recombination sites.Type: ApplicationFiled: December 1, 2004Publication date: September 22, 2005Applicant: Invitrogen CorporationInventors: Jon Chesnut, Louis Leong