Abstract: The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be assayed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptide having a detectable activity.

Abstract: The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention.

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.

Abstract: The present invention relates to the fields of biotechnology and molecular biology. In particular, the present invention relates to methods for preparing marker molecules for identifying physical properties of molecular species separated by electrophoretic systems. The methods comprise joining multiple nucleic acid segments containing at least one recombination site under conditions favoring recombination. The present invention relates to both nucleic acid marker molecules and protein marker molecules, compositions comprising said marker molecules, and methods for preparing said marker molecules.

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different.

Abstract: A programmable logic array integrated circuit device has a relatively large block of programmable memory cells in addition to the usual programmable logic modules and the usual programmable interconnection conductor network. In order to simplify the circuitry associated with the large block, and especially the circuitry for addressing that block during programming and/or verification of the device, the address decoder that is normally used to address the block during use of the device to perform logic is also used during programming and/or verification. During programming and/or verification a counter or other similar coded address signal generating circuitry is used to supply address information to the decoder.