Patents by Inventor Luhan Yang
Luhan Yang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10925263Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: GrantFiled: December 10, 2019Date of Patent: February 23, 2021Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Marc Guell
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Publication number: 20200404891Abstract: The present disclosure provides methods of generating multiplexed genetically modified animals, for example, porcine endogenous retrovirus (PERV)-inactivated pigs. The disclosure also provides methods of improving the birth rate of multiplexed genetically modified animals. In some embodiments, the present closure is concerned with the generation and utilization of porcine cells in which porcine endogenous retroviral (PERV) elements have been inactivated. In sonic embodiments, the PERV-free or PERV-reduced porcine cells are cloned to produce porcine embryos. In some embodiments, the PERV-free or PERV-reduced embryos may be grown into adult swine from which organs and/or tissues may be extracted and used for such purposes as xenotransplantation into non-porcine animals such as humans.Type: ApplicationFiled: April 20, 2018Publication date: December 31, 2020Inventor: Luhan Yang
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Publication number: 20200308599Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: ApplicationFiled: May 27, 2020Publication date: October 1, 2020Inventors: George M. Church, Prashant G. Mali, Luhan Yang
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Patent number: 10717990Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: GrantFiled: June 30, 2014Date of Patent: July 21, 2020Assignee: President and Fellows of Harvard CollegeInventors: Prashant G. Mali, George M. Church, Luhan Yang
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Publication number: 20200170229Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: ApplicationFiled: December 10, 2019Publication date: June 4, 2020Inventors: George M. Church, Luhan Yang, Marc Guell
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Patent number: 10563225Abstract: Methods are provided for the use of Cas9 in genome engineering of stem cells. Methods include introducing into the stem cell a first foreign nucleic acid encoding a guide RNA complementary to a target DNA and which guides a Cas9 enzyme to the target DNA, wherein the RNA and the enzyme are members of a co-localization complex for the target DNA, introducing into the stem cell a second foreign nucleic acid encoding a donor nucleic acid sequence, wherein the guide RNA and the donor nucleic acid sequences are expressed, wherein the guide RNA and the Cas 9 enzyme co-localize to the target DNA, the Cas 9 enzyme cleaves the target DNA and the donor nucleic acid is inserted into the target DNA to produce altered DNA in the stem cell.Type: GrantFiled: June 30, 2014Date of Patent: February 18, 2020Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Marc Guell, Joyce Lichi Yang
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Publication number: 20200048656Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: ApplicationFiled: June 13, 2019Publication date: February 13, 2020Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Prashant G. Mali, Luhan Yang
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Publication number: 20190350177Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: ApplicationFiled: August 5, 2019Publication date: November 21, 2019Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Marc Guell
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Publication number: 20190352670Abstract: The present disclosure provides methods of improving nuclease-mediated gene targeting frequencies in cells using PFT? or bFGF.Type: ApplicationFiled: January 31, 2018Publication date: November 21, 2019Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang
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Patent number: 10435708Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: GrantFiled: February 12, 2016Date of Patent: October 8, 2019Assignee: President and Fellows of Harvard CollegeInventors: Prashant G. Mali, George M. Church, Luhan Yang
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Publication number: 20190249194Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: ApplicationFiled: April 29, 2019Publication date: August 15, 2019Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Prashant G. Mali, Luhan Yang
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Publication number: 20190249193Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: ApplicationFiled: April 29, 2019Publication date: August 15, 2019Inventors: George M. Church, Prashant G. Mali, Luhan Yang
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Patent number: 10375938Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: GrantFiled: February 5, 2018Date of Patent: August 13, 2019Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Marc Guell
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Patent number: 10273501Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: GrantFiled: July 2, 2015Date of Patent: April 30, 2019Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Prashant G. Mali, Luhan Yang
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Publication number: 20190062739Abstract: Methods and compositions of altering mitochondrial DNA of a eukaryotic cell are provided using one or more of a mitochondrial specific adeno-associated virus to deliver one or more nucleic acids encoding CRISPR system including a Cas9 protein or its nuclease inactive variant and a guide RNA into a mitochondria for expression within the mitochondria. The Cas9 system can cut, nick or regulate a target mitochondrial nucleic acid.Type: ApplicationFiled: February 2, 2017Publication date: February 28, 2019Applicant: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Margo R. Monroe
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Publication number: 20180288983Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: ApplicationFiled: October 7, 2016Publication date: October 11, 2018Inventors: George M. Church, Luhan Yang, Marc Guell
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Publication number: 20180171361Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.Type: ApplicationFiled: February 5, 2018Publication date: June 21, 2018Inventors: George M. Church, Luhan Yang, Marc Guell
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Patent number: 9970024Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: GrantFiled: December 16, 2013Date of Patent: May 15, 2018Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Prashant Mali, Luhan Yang
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Patent number: 9914939Abstract: Methods of genome engineering in cells using a TALEN lacking repeat sequences or Cas9 is provided.Type: GrantFiled: July 25, 2014Date of Patent: March 13, 2018Assignee: President and Fellows of Harvard CollegeInventors: George M. Church, Luhan Yang, Marc Guell, Joyce Lichi Yang
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Publication number: 20170044569Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.Type: ApplicationFiled: April 8, 2015Publication date: February 16, 2017Inventors: George M. Church, Prashant Mali, Luhan Yang