Abstract: A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium, which comprises steps of:(1) preparing at least two one-dimensional waveforms composed of signal position along the resolving direction and signal level for each band;(2) detecting a position where signal level is maximum on one of the one-dimensional waveforms;(3) comparing the signal level at said maximum position on the one-dimensional waveform with signal levels at the corresponding positions on one-dimensional waveforms adjacent thereto, to determine a maximum position on the one-dimensional waveform having the highest signal level to be a band position;(4) detecting a subsequent position where signal level is maximum

Abstract: In a method of reading out a radiation image stored in a stimulable phosphor sheet, preliminary read-out for approximately detecting the image is conducted. A cumulative histogram of the image information is created, read-out conditions in the final read-out are corrected based on a characteristic value of the cumulative histogram, then the final read-out is conducted using the corrected conditions. Alternatively, a standard image information range is determined from the characteristic value, and the read-out conditions are adjusted so that the standard range corresponds to a predetermined electronic signal range. In addition, in the read-out method where gradation processing is performed for electronic image signals obtained by photoelectrically detecting the phosphor light emitted by the sheet, histogram of the image signals is created, and a desired image signal range is determined therefrom.

Abstract: A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium,which comprises steps of:(1) detecting at least two bands continuously in the lower part of each resolved row and numbering the bands consecutively in order from the lower end;(2) obtaining correlation of a distance between the detected bands in the resolving direction with the band's number and predicting positions of undetected bands in the resolving direction from the correlation;(3) detecting at least one band on the resolved rows on the basis of the predicted positions and numbering the bands consecutively;(4) obtaining the correlation of the distance between the bands with the band's number for the already detected ban

Abstract: A signal processing method for analyzing an autoradiograph by subjecting digital image data containing an autoradiograph of a resolved pattern to digital signal processing to obtain locational information on radioactively labeled substances in the form of numerals and/or symbols is disclosed. The resolved pattern is formed by resolving radioactively labeled substances, such as, for example, nucleic acids, in a one-dimensional direction on a support medium. The total boundary area of the resolved patterns throughout the digital image data is determined by first dividing the digital image data into two or more blocks along a direction perpendicular to the resolving direction and then preparing a waveform composed of positions along the direction perpendicular to the resolving direction for each block by compiling the digital image date for each block.

Abstract: A signal processing method for analyzing an autoradiograph to identify the locations of radioactively labeled substances which have been resolved in a one-dimensional developing direction on a support medium is provided. According to this method, digital image data including a plurality of different levels and values obtained by placing the support medium and a radiographic film together in layers to record the autoradiograph of the radioactively labeled substances are subjected to digital signal processing to identify their positions in a manner which substantially eliminates background noise. A stimulable phosphor sheet can be used in place of the radiographic film to record the autoradiograph. The method described herein may be effectively used to identify selected components of various biopolymers.

Abstract: A signal processing method for determining the base sequence of nucleic acids from data on an autoradiograph is provided. An autoradiograph including a plurality of resolved rows is formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments that have been labeled with a radioactive element in a one-dimensional direction on a support medium. The method detects bands along each resolved row corresponding to the nucleic acid bases from digital signal data representing position and signal level for each row. The digital signal data corresponding to the autoradiograph may be photoelectrically detected as stimulated emission from the recording of the autoradiograph of the resolved rows on a phosphor sheet or as visible images from the recording by the autoradiograph of the resolved rows on a radiosensitive material.

Abstract: A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of a resolved pattern which is formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium, which comprises steps of:(1) preparing at least two one-dimensional waveforms for each band, with position along the resolving direction as abscissa and signal level as ordinate;(2) detecting positions at which signal level is maximum on each waveform; and(3) comparing the positions having maximum signal level detected on the plural waveforms for each band to determine a position of said band.

Abstract: The base sequence of nucleic acids is determined by subjecting digital signals to signal processing, where the digital signals correspond to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium.The method involves detecting at least two bands in the lower part of each resolved row and numbering the bands consecutively from the lower end, obtaining correlation between the band's number and a resolved distance thereof for each resolved row, and then determining the difference in the resolved distance between the resolved rows from the resulting correlation and making correction for the resolved position on each row by taking the difference as a locational deviation of the rows from each other.

Abstract: A seabed mound creating method suitable for a construction of a large scale offshore structure comprises the steps of setting up a plurality of sheet piles along the outer periphery of a mound creation region in a seabed area of soft ground to form an earth-retaining wall projecting from the seabed, and raising the ground level of the seabed within the earth-retaining wall by depositing soft soil into the earth-retaining wall to heap the deposited soil to a predetermined height. The method further comprises the step of implementing a ground improvement process to both the heaped-up soft soil and the soft ground of the seabed within the earth-retaining wall so that the ground improvement process is provided to a predetermined depth below the seabed, thus to form a seabed mound integral with the improved ground. The seabed mound may be provided at the upper portion with a projection area serving as a shear key with respect to a horizontal external force.