Abstract: The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components.

Abstract: The present invention provides a method for transforming an insect genome that has a much enhanced transformation frequency. The vectors and plasmids employed in the method are further described as transposition vectors that include a minimal amount of nucleotide sequence homologous to a 5? region and a 3? region of a native piggyBac nucleic acid sequence. The transformed cells or embryos may also be developed into transgenic organisms. Disclosed are minimal piggyBac-based plasmid constructs that comprises a minimal nucleic acid sequence homologous to a 5? end of a piggyBac nucleic acid sequence (about 60-80 bp, particularly 66 bp) and a relatively long (300 to about 380 bp, particularly 311 bp or 378 bp) continuous nucleic acid sequence homologous to a 3? end of a piggyBac native nucleic acid sequence. Methods employing these constructs include the use of a helper plasmid.

Abstract: The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components.

Abstract: The present invention relates to DNAzymes (also known as deoxyribozymes, DNA enzymes, catalytic DNA, or DZ), which are conjugated to nanoparticles (NP) to facilitate the detection of nucleic acids. One aspect of the invention relates to compounds comprising DNAzymes conjugated to nanoparticles (DZ-NP), such as metallic or gold nanoparticles, and methods for their synthesis. Another aspect of the invention relates to methods of using the conjugated compounds to detect nucleic acids, such as genomic material or transcripts of infectious agents, such as viruses, exemplified by applications demonstrating visual detection of Flavivirus RNA molecules, such as dengue virus, or Alphavirus RNA molecules, such as chikungunya virus, in short time periods, using compositions comprising stable components.

Abstract: A transgenic silkworm system for recombinant glycoprotein production is provided.

Abstract: Disclosed are anto-DENV ribozyme based methods and compositions useful in the inhibition and control of all Dengue fever serotypes (designated DENV 1 through 4). A group of anti-DENV Group 1 trans-splicing introns (?DENV-GrpIa) are presented that target DENV-2 NGC genomes in situ. Methods for specifically targeting a highly conserved 5?-3? cyclization sequence (CS) region that is common to all serotypes of the DENV are provided. The anti-DENV Group 1 trans-splicing introns (?DENV-GrpIa) specifically target two different uracil bases on the positive sense genomic strand. The invention provides an RNA based approach for transgeneic suppression of DENV in transformed mosquitoes using a group of specifically designed introns that trans-splice a new RNA sequence downstream of a targeted site. The aDENV-GrpIs target DENV infected genomes and thus provide a method for inhibiting the spread of Dengue fever.

Abstract: The present invention provides molecular methods for efficiently transforming the genome of common disease-transmitting parasites, such as Plasmodium falciparum. The transformation efficiencies are improved up to 100 times over those conventionally known. The methods provide high saturation of the target parasite genome, of 50% or greater, and target non-specifically TTAA-rich sites in the parasite genome. The invention also discloses a model that may be used to functionally annotate the genome of the Plasmodium falciparum, thus permitting the design and screening of compounds that may be useful in the control and inhibiting of diseases caused and transmitted by these parasites, including malaria. Highly efficient and multi-site integrating transposons, particularly piggyBac transposons, which provide for random and multi-site integration into parasite genomes in the presence of a helper plasmid, are also presented.

Abstract: The present invention provides efficient transfer of genes into host cells or embryos to transform the cells or embryos by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may also be developed into transgenic organisms.

Abstract: More efficient transfer of genes into host cells or embryos to transform the cells or embryos is facilitated by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may be developed into transgenic organisms.

Abstract: The present invention is directed to nucleic acid and amino acid sequences for transformation constructs containing piggyBac or tagalong transposable elements. These constructs allow for the precise excision and insertion of heterologous DNA into a host cell.

Abstract: More efficient transfer of genes into host cells or embryos to transform the cells or embryos is facilitated by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may be developed into transgenic organisms.

Abstract: The present invention is directed to nucleic acid and amino acid sequences for transformation constructs containing piggyBac or tagalong transposable elements. These constructs allow for the precise excision and insertion of heterologous DNA into a host cell.

Abstract: The present invention relates to recombinant vector/host systems which can direct the expression of foreign genes under the control of the Heliothis polyhedrin promoter. Using the systems of the present invention, a heterologous gene of interest can be expressed as an unfused peptide or protein, a fusion protein, or as a recombinant occlusion body which comprises crystallized polyhedrin fusion proteins bearing the heterologous gene product on the surface of or within the occlusion body. The recombinant proteins or occlusion bodies of the present invention have uses in vaccine formulations and immunoassays, as biological insecticides, and as expression systems for the production of foreign peptides or proteins.

Abstract: The present invention is directed to recombinant baculoviruses which encode fusion polyhedrin proteins capable of forming occlusion bodies containing foreign peptides. The recombinant baculoviruses of the invention are formed by insertion into or replacement of regions of the polyhedrin gene that are not essential for occlusion body formation, with foreign DNA fragments by recombinant DNA techniques. The recombinant occlusion bodies produced in accordance with the present invention have uses in vaccine formulations, immunoassays, immobilized enzyme reactions, as biological insecticides, and as expression vectors.

Abstract: A method of cladding a medium or high steel core rod with another metal includes a hot water quench at a temperature of about 160.degree. F. to boiling temperature and preferably about 195.degree. F. to 205.degree. F. with a subsequent quench in water at a temperature below a level of the first temperature and preferably at about ambient temperature. The clad material is found to be substantially devoid of objectionable oxide particles or surface sponge particles and the steel core substantially devoid of acicular transformation products permitting continued processing by deformation processes, such as wire drawing.

Abstract: A method of cladding a nonaluminum core with at least one aluminum cladding strip by preheating both the core rod and the strip with the former being preheated to about 1000.degree. F. to 1300.degree. F. and the latter being heated to about 600.degree. F. to 1000.degree. F. Cleaning the core rod and the strip and passing them through a controlled environment chamber containing a reducing or neutral gas. Lubricating the bonding roll grooves to provide a substantially continuous coating of lubricant thereon. Galling of the aluminum strip or strips to the bonding rolls is resisted as a result of this process.

Abstract: The specification discloses manufacture of clad rod and the like by rolling cladding strip on to the core rod. By matching the core rod cross section, cladding strip cross section, and roll pass design a composite clad rod of improved concentricity may be produced.

Abstract: A metal rod is supplied to a pair of bonding rolls provided with peripheral grooves forming a substantially circular roll pass that also receives at opposite sides of the rod a pair of metal strips of a different metal than that of the rod. The rolls press the strips against the rod and pull all three together through the roll pass, with the strips bent around the rod to enclose it. Before the strips reach the pass, an electric current passes through a length of each one to heat it to a solid-phase bonding temperature and to burn off contaminants. Also, before the rod reaches the rolls, it is heated to a solid-phase bonding temperature by means of an electric induction coil encircling it while it is passing through an enclosure maintained full of a gas providing a controlled atmosphere around the heated portion of the rod. The diameter of the roll pass is small enough to reduce the diameter of the product therein and simultaneously cause solid-phase bonding together of the heated strips and heated rod.