Patents by Inventor Manabu Tokeshi

Manabu Tokeshi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210341488
    Abstract: A fluorescence polarization immunoassay that uses a fluorescent labeling substance in which a single domain antibody is labeled with a fluorescent dye. A fluorescent labeling substance obtained by labeling a single domain antibody, such as a VHH antibody or a vNAR antibody, having a binding ability to a target substance with a fluorescent dye is used. The fluorescence polarization immunoassay includes a binding step for binding the fluorescent labeling substance to a target substance; and a measuring step for measuring a change in the fluorescence polarization of the fluorescent labeling substance to which the target substance is bound.
    Type: Application
    Filed: April 27, 2021
    Publication date: November 4, 2021
    Applicants: TOHOKU UNIVERSITY, Tianma Japan, Ltd., NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Mao Fukuyama, Akihide Hibara, Ayuko Imai, Koji Shigemura, Manabu Tokeshi
  • Publication number: 20210129103
    Abstract: Provided are: a flow channel structure with which lipid particles or micelles, which are useful as nano-sized carriers, for example, in drug delivery systems, are produced with good control of particle size; and a method for forming lipid particles or micelles using the same. Said flow channel structure has a two-dimensional structure such as one in which multiple structural elements (baffles) of a specified width are alternately disposed from the two side faces in a micro-sized flow channel through which feedstock solutions are flowed.
    Type: Application
    Filed: April 13, 2018
    Publication date: May 6, 2021
    Inventors: Manabu Tokeshi, Masatoshi Maeki, Yusuke Sato, Hideyoshi Harashima
  • Publication number: 20180080929
    Abstract: The present invention provides a device capable of detecting a plurality of items in a specimen using a single optical system. Microstructures holding specific binding reagents in a photocured hydrophilic resin are arranged in a channel, whereby the reagents do not mix during manufacture of the device, and a device capable of multiplex detection can be manufactured.
    Type: Application
    Filed: March 17, 2016
    Publication date: March 22, 2018
    Inventors: Toshihiro KASAMA, Yoshinobu BABA, Manabu TOKESHI, Nanako NISHIWAKI
  • Patent number: 9719927
    Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1, and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.
    Type: Grant
    Filed: May 8, 2015
    Date of Patent: August 1, 2017
    Assignees: TOHOKU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Akihide Hibara, Manabu Tokeshi, Osamu Wakao
  • Publication number: 20170074796
    Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1 and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.
    Type: Application
    Filed: May 8, 2015
    Publication date: March 16, 2017
    Inventors: Akihide HIBARA, Manabu TOKESHI, Osamu WAKAO
  • Patent number: 8920845
    Abstract: It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.
    Type: Grant
    Filed: November 2, 2007
    Date of Patent: December 30, 2014
    Assignee: National University Corporation Nagoya University
    Inventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Hideyoshi Harashima, Kentaro Kogure, Yasuo Shinohara, Hiroshi Kuramoto
  • Patent number: 8802449
    Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.
    Type: Grant
    Filed: December 25, 2006
    Date of Patent: August 12, 2014
    Assignees: Institute of Microchemical Technology, Kansai Paint Co., Ltd.
    Inventors: Masaya Kakuta, Manabu Tokeshi, Hitoshi Izumida, Takahide Takadera, Kenji Miyagawa
  • Patent number: 8420891
    Abstract: Disclosed is a method for introducing a foreign substance (3) into a cell (1) having a cell wall. The method comprises the steps of: providing a carbon nanotube (2) carrying at least one enzyme capable of decomposing a cell wall; supplying the carbon nanotube (2) and the foreign substance (3) into a treatment solution containing the cell (1); decomposing the cell wall of the cell (1) by the action of the enzyme carried on the carbon nanotube (2) upon the contact of the carbon nanotube (2) with the cell (1); and introducing the foreign substance (3) into the cell (1) through a site decomposed with the enzyme on the cell wall.
    Type: Grant
    Filed: June 11, 2008
    Date of Patent: April 16, 2013
    Assignee: National University Corporation Nagoya University
    Inventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Ibrahim Maged Fouad Serag Eldin Bayoumi
  • Publication number: 20110038758
    Abstract: A microchip, which is used in a diagnostic system using a microfluid system, has a flow path capable of greatly improving the reaction efficiency and realizing a stable measurement with high reproducibility. The microchip has two substrates with at least a flow path 12 formed at the interface between the two substrates, the flow path 12 having a reaction area 14 and a detection area 15 downstream of the reaction area 14, and the flow path 12 at the detection area 15 having a depth which is deeper than the flow path 12 in the reaction area 14.
    Type: Application
    Filed: November 18, 2005
    Publication date: February 17, 2011
    Applicant: Nissui Pharmaceutical Co., Ltd.
    Inventors: Shuichi Akaba, Yuichi Oku, Manabu Tokeshi, Takehiko Kitamori
  • Publication number: 20100261205
    Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.
    Type: Application
    Filed: December 25, 2006
    Publication date: October 14, 2010
    Inventors: Masaya Kakuta, Manabu Tokeshi, Hitoshi Izumida, Takahide Takadera, Kenji Miyagawa
  • Publication number: 20100248373
    Abstract: Disclosed is a method for introducing a foreign substance (3) into a cell (1) having a cell wall. The method comprises the steps of: providing a carbon nanotube (2) carrying at least one enzyme capable of decomposing a cell wall; supplying the carbon nanotube (2) and the foreign substance (3) into a treatment solution containing the cell (1); decomposing the cell wall of the cell (1) by the action of the enzyme carried on the carbon nanotube (2) upon the contact of the carbon nanotube (2) with the cell (1); and introducing the foreign substance (3) into the cell (1) through a site decomposed with the enzyme on the cell wall.
    Type: Application
    Filed: June 11, 2008
    Publication date: September 30, 2010
    Applicant: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
    Inventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Ibrahim Maged Fouad Serag Eldin Bayoumi
  • Publication number: 20100143482
    Abstract: It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.
    Type: Application
    Filed: November 2, 2007
    Publication date: June 10, 2010
    Applicant: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
    Inventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Hideyoshi Harashima, Kentaro Kogure, Yasuo Shinohara, Hiroshi Kuramoto
  • Patent number: 7695684
    Abstract: For enlarging the application range of samples capable of being treated, decreasing the amount of samples used, preventing deterioration of samples by a change with the passage of time and preventing mixing of samples by diffusion, a micro fluidics system according to the present invention comprises a sample server which stores plural samples and which has sample efflux portions, a microchip for feeding samples to a treating portion through plural sample introducing portions communicating with the sample efflux portions and further through a microchannel, allowing the samples to be subjected to a predetermined treatment, and a sample feeder for feeding the samples stored in the sample server to the sample introducing portions through the sample efflux portions.
    Type: Grant
    Filed: May 27, 2005
    Date of Patent: April 13, 2010
    Assignee: Hitachi Plant Technologies, Ltd.
    Inventors: Hidekazu Tsudome, Takehiko Kitamori, Manabu Tokeshi
  • Publication number: 20100060981
    Abstract: An objective of the present invention is to provide a circular dichroism thermal lens microscope apparatus capable of identifying and quantifying optically active samples in ultra-trace amounts, and which has a higher sensitivity than conventional apparatuses. The objective is achieved by a circular dichroism thermal lens microscope apparatus which beams excitation light and detection light into an optical microscope, where the detection light enters a thermal lens formed by irradiating a sample with the excitation light, and a substance in a sample is detected by determining the diffusion of the detection light by the thermal lens, and where the excitation light is modulated by a phase-modulation element, so as to identify or quantify an optical isomer.
    Type: Application
    Filed: February 10, 2006
    Publication date: March 11, 2010
    Applicants: Kanagawa Academy of Science and Technology, Institute of Microchemical Technology, The University of Tokyo
    Inventors: Masayo Yamauchi, Akihide Hibara, Takehiko Kitamori, Kazuma Mawatari, Manabu Tokeshi
  • Patent number: 7604406
    Abstract: A plurality of elements can be analyzed simultaneously with high sensitivity using a microchip. The microchip (1) comprises a substrate (30), a channel (23) formed in the substrate (30), and an analyzing part (10) consisting of a part of flat surface of the substrate (30), where the outlet of the channel (23) is formed as an opening (9c) and measurement object liquid overflowed from the opening (9c) stays on the flat surface of the substrate (30) to become a sample of analysis. The measurement object liquid is made to overflow as a sample of analysis to the analyzing part (10) using the microchip (1) and then the sample of analysis is preferably dried before a primary X-ray is made to enter under conditions of total reflection and fluorescent X-rays are detected.
    Type: Grant
    Filed: August 30, 2006
    Date of Patent: October 20, 2009
    Assignee: Japan Science and Technology Agency
    Inventors: Kouichi Tsuji, Takehiko Kitamori, Manabu Tokeshi, Keita Tanaka, Kazuhiko Nakano
  • Publication number: 20090147912
    Abstract: A plurality of elements can be analyzed simultaneously with high sensitivity using a microchip. The microchip (1) comprises a substrate (30), a channel (23) formed in the substrate (30), and an analyzing part (10) consisting of a part of flat surface of the substrate (30), where the outlet of the channel (23) is formed as an opening (9c) and measurement object liquid overflowed from the opening (9c) stays on the flat surface of the substrate (30) to become a sample of analysis. The measurement object liquid is made to overflow as a sample of analysis to the analyzing part (10) using the microchip (1) and then the sample of analysis is preferably dried before a primary X-ray is made to enter under conditions of total reflection and fluorescent X-rays are detected.
    Type: Application
    Filed: August 30, 2006
    Publication date: June 11, 2009
    Inventors: Kouichi Tsuji, Takehiko Kitamori, Manabu Tokeshi, Keita Tanaka, Kazuhiko Nakano
  • Publication number: 20090035793
    Abstract: In a microchip which enables cell cultivation and accurate cell count measurement, fine particles affixed with cells are trapped within a passage by making the minimum width of a solution and fine particle inlet into a cell culture portion larger than the maximum diameter of the fine particles, and making the width of an outlet smaller than the maximum diameter of the fine particles.
    Type: Application
    Filed: August 31, 2006
    Publication date: February 5, 2009
    Applicants: Nissan Chemical Industries, Ltd., Kanagawa Academy of Science and Technology
    Inventors: Taito Nishino, Masaki Kitahara, Manabu Tokeshi, Takehiko Kitamori
  • Patent number: 7402439
    Abstract: An integrated structure of multilayer flow microchannel, which comprises a plurality of microchannels in which parallel multilayer flow that involves the interface of fluids is formed, arranged on various positions of a substrate, wherein each of the multilayer flow microchannels is communicated with another multilayer flow microchannel. A plurality of unit operations can be carried out continuously with high efficiency on a microchip, enabling high-degree microanalysis or precise chemical synthesis using the highly integrated structure of multilayer flow microchannel.
    Type: Grant
    Filed: July 10, 2002
    Date of Patent: July 22, 2008
    Assignee: Kanagawa Academy of Science and Technology
    Inventors: Takehiko Kitamori, Manabu Tokeshi, Akihide Hibara
  • Publication number: 20080123088
    Abstract: There are provided a microchemical system capable of acquiring a highly accurate TLM output value and a method for calculating TLM output thereof. A microchemical system 1 comprises: a microchemical chip having a channel with a depth t in which a sample flows; an exciting light source 13 adapted to irradiate the sample with an exciting light through an objective lens 10 with a numerical aperture NA; a detecting light source 14 adapted to irradiate the sample with a detecting light coaxially with the exciting light through the objective lens 10; and a PD adapted to receive a transmitted light when the detecting light transmits the sample before and after formation of a thermal lens 12. When a TLM output is calculated in the microchemical system 1 on the basis of a received light amount of the PD, the depth t (?m) is set to the range of 75?t?300, the numerical aperture NA is set to the range of 0.04?NA?0.
    Type: Application
    Filed: November 16, 2007
    Publication date: May 29, 2008
    Applicant: Nippon Sheet Glass Company, Limited
    Inventors: Masatoshi Nara, Ryo Anraku, Takahiro Asai, Jun Yamaguchi, Akihiko Hattori, Takehiko Kitamori, Manabu Tokeshi, Akihide Hibara, Kazuma Mawatari
  • Publication number: 20080030735
    Abstract: A desktop thermal lens microscope apparatus can detect a chemical reaction in a micro spatial region within a glass chip, or the like, more conveniently and at any given place. The desktop thermal lens microscope apparatus has small laser light sources such as semiconductor lasers as an excitation light source and a probe light source. Since an excitation light source system (P), a probe light source (Q), and a thermal lens microscope optical system (R, S, T) are integrally provided in a single housing, the size of the desktop thermal lens microscope is as small as that of a desktop microscope.
    Type: Application
    Filed: February 5, 2007
    Publication date: February 7, 2008
    Inventors: Takehiko Kitamori, Manabu Tokeshi, Akira Tsunemi