Abstract: The present invention pertains to a flow path structure body for forming self-assembling molecular particles. The flow path structure body has a base body and a flow path structure provided to the interior thereof, the flow path structure having a first introduction channel 10 and a second introduction channel 20 that are independent of one another on the upstream side of the flow path structure, and the introduction channels merging at a merging site. The flow path structure has a dilution flow path 40 that is bent three-dimensionally toward the downstream side of the merging site. The dilution flow path 40 has two or more Y structural elements 50 that protrude out in the Y direction and one or more Z structural elements 60 that protrude out in the Z direction within the dilution flow path, and at least two adjacent Y structural elements protrude out alternately in the Y direction.

Abstract: The present invention addresses the problem of providing a lipid nanoparticle in which a nucleic acid, etc., required in genome editing is encapsulated and which can be produced by an alcohol dilution method using flow channels and contributes to high genome editing efficiency. The present invention pertains to a lipid nanoparticle which comprises a lipid component, a DNA nuclease, a guide RNA and a single-stranded oligonucleotide, wherein: the lipid component comprises a pH-sensitive cationic lipid, a neutral phospholipid and a polyalkylene glycol-modified lipid; the ratio of the pH-sensitive cationic lipid relative to the total lipids constituting the lipid nanoparticle is 30-50 mol %; the ratio of the neutral phospholipid relative to the total lipids constituting the lipid nanoparticle is 20-50 mol %; and the ratio of the polyalkylene glycol-modified lipid relative to the total lipids constituting the lipid nanoparticle is 1-4 mol %.

Abstract: A fluorescence polarization immunoassay that uses a fluorescent labeling substance in which a single domain antibody is labeled with a fluorescent dye. A fluorescent labeling substance obtained by labeling a single domain antibody, such as a VHH antibody or a vNAR antibody, having a binding ability to a target substance with a fluorescent dye is used. The fluorescence polarization immunoassay includes a binding step for binding the fluorescent labeling substance to a target substance; and a measuring step for measuring a change in the fluorescence polarization of the fluorescent labeling substance to which the target substance is bound.

Abstract: Provided are: a flow channel structure with which lipid particles or micelles, which are useful as nano-sized carriers, for example, in drug delivery systems, are produced with good control of particle size; and a method for forming lipid particles or micelles using the same. Said flow channel structure has a two-dimensional structure such as one in which multiple structural elements (baffles) of a specified width are alternately disposed from the two side faces in a micro-sized flow channel through which feedstock solutions are flowed.

Abstract: The present invention provides a device capable of detecting a plurality of items in a specimen using a single optical system. Microstructures holding specific binding reagents in a photocured hydrophilic resin are arranged in a channel, whereby the reagents do not mix during manufacture of the device, and a device capable of multiplex detection can be manufactured.

Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1, and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.

Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1 and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.

Abstract: It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.

Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.

Abstract: Disclosed is a method for introducing a foreign substance (3) into a cell (1) having a cell wall. The method comprises the steps of: providing a carbon nanotube (2) carrying at least one enzyme capable of decomposing a cell wall; supplying the carbon nanotube (2) and the foreign substance (3) into a treatment solution containing the cell (1); decomposing the cell wall of the cell (1) by the action of the enzyme carried on the carbon nanotube (2) upon the contact of the carbon nanotube (2) with the cell (1); and introducing the foreign substance (3) into the cell (1) through a site decomposed with the enzyme on the cell wall.

Abstract: A microchip, which is used in a diagnostic system using a microfluid system, has a flow path capable of greatly improving the reaction efficiency and realizing a stable measurement with high reproducibility. The microchip has two substrates with at least a flow path 12 formed at the interface between the two substrates, the flow path 12 having a reaction area 14 and a detection area 15 downstream of the reaction area 14, and the flow path 12 at the detection area 15 having a depth which is deeper than the flow path 12 in the reaction area 14.

Abstract: An objective of the present invention is to provide immunoassay microchips in which microstructures of beads having a sufficient reaction area were constructed within microchannels while suppressing flow path resistance, and to provide simple and highly-sensitive immunoassay methods for microsamples. The objective was achieved by immunoassay microchips comprising microchannels with microstructures arranged in at least a portion of the microchannels, the microstructures retaining microbeads uniformly dispersed in photo-cured hydrophilic resins, and the microbeads having a primary antibody immobilized on their surfaces, and by immunoassay methods using the microchips.

Abstract: Disclosed is a method for introducing a foreign substance (3) into a cell (1) having a cell wall. The method comprises the steps of: providing a carbon nanotube (2) carrying at least one enzyme capable of decomposing a cell wall; supplying the carbon nanotube (2) and the foreign substance (3) into a treatment solution containing the cell (1); decomposing the cell wall of the cell (1) by the action of the enzyme carried on the carbon nanotube (2) upon the contact of the carbon nanotube (2) with the cell (1); and introducing the foreign substance (3) into the cell (1) through a site decomposed with the enzyme on the cell wall.

Abstract: It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.

Abstract: For enlarging the application range of samples capable of being treated, decreasing the amount of samples used, preventing deterioration of samples by a change with the passage of time and preventing mixing of samples by diffusion, a micro fluidics system according to the present invention comprises a sample server which stores plural samples and which has sample efflux portions, a microchip for feeding samples to a treating portion through plural sample introducing portions communicating with the sample efflux portions and further through a microchannel, allowing the samples to be subjected to a predetermined treatment, and a sample feeder for feeding the samples stored in the sample server to the sample introducing portions through the sample efflux portions.

Abstract: An objective of the present invention is to provide a circular dichroism thermal lens microscope apparatus capable of identifying and quantifying optically active samples in ultra-trace amounts, and which has a higher sensitivity than conventional apparatuses. The objective is achieved by a circular dichroism thermal lens microscope apparatus which beams excitation light and detection light into an optical microscope, where the detection light enters a thermal lens formed by irradiating a sample with the excitation light, and a substance in a sample is detected by determining the diffusion of the detection light by the thermal lens, and where the excitation light is modulated by a phase-modulation element, so as to identify or quantify an optical isomer.

Abstract: A plurality of elements can be analyzed simultaneously with high sensitivity using a microchip. The microchip (1) comprises a substrate (30), a channel (23) formed in the substrate (30), and an analyzing part (10) consisting of a part of flat surface of the substrate (30), where the outlet of the channel (23) is formed as an opening (9c) and measurement object liquid overflowed from the opening (9c) stays on the flat surface of the substrate (30) to become a sample of analysis. The measurement object liquid is made to overflow as a sample of analysis to the analyzing part (10) using the microchip (1) and then the sample of analysis is preferably dried before a primary X-ray is made to enter under conditions of total reflection and fluorescent X-rays are detected.

Abstract: A plurality of elements can be analyzed simultaneously with high sensitivity using a microchip. The microchip (1) comprises a substrate (30), a channel (23) formed in the substrate (30), and an analyzing part (10) consisting of a part of flat surface of the substrate (30), where the outlet of the channel (23) is formed as an opening (9c) and measurement object liquid overflowed from the opening (9c) stays on the flat surface of the substrate (30) to become a sample of analysis. The measurement object liquid is made to overflow as a sample of analysis to the analyzing part (10) using the microchip (1) and then the sample of analysis is preferably dried before a primary X-ray is made to enter under conditions of total reflection and fluorescent X-rays are detected.

Abstract: In a microchip which enables cell cultivation and accurate cell count measurement, fine particles affixed with cells are trapped within a passage by making the minimum width of a solution and fine particle inlet into a cell culture portion larger than the maximum diameter of the fine particles, and making the width of an outlet smaller than the maximum diameter of the fine particles.

Abstract: An integrated structure of multilayer flow microchannel, which comprises a plurality of microchannels in which parallel multilayer flow that involves the interface of fluids is formed, arranged on various positions of a substrate, wherein each of the multilayer flow microchannels is communicated with another multilayer flow microchannel. A plurality of unit operations can be carried out continuously with high efficiency on a microchip, enabling high-degree microanalysis or precise chemical synthesis using the highly integrated structure of multilayer flow microchannel.