Abstract: Diversified grafts suitable for use in repair and reconstruction procedures are provided. Some diversified grafts comprise two or more heterogenous features which form regions having different preferred properties. A diversified graft useful for pre-pectoral breast reconstruction, meshing is provided on an upper pole of the diversified graft and a plurality of slits arranged in particular patterns is provided on a lower pole of the graft, whereby the upper pole has a greater expansion ability and the lower pole has lesser degree of expansion ability but maintains greater load bearing capacity which is necessary to support the lower pole of the breast undergoing reconstruction, while allowing for fluid egress. In other embodiments, the diversified graft comprises two or more components which are combined by attaching them to each other to form a larger graft of the required size. The components may be smaller pieces of the same material or different materials.

Abstract: Multiple component grafts are provided for treatment of tissue defects and comprise two or more components, each of which is a tissue-derived matrix and at least two of which are derived from different types of tissue. For example, a first component may be a matrix derived from cartilage tissue such as cartilage fibers with or without viable cells, cartilage particles with or without viable cells, or combinations of any two or more such cartilage-derived matrices. A second component may be a matrix derived from bone tissue such as mineralized or demineralized cortical bone fibers, viable cancellous bone matrix (e.g., cryopreserved or lyophilized chips, particulates, powder, sheets, putty, flowable fluid, etc.), demineralized or demineralized cancellous bone matrix (chips, particulates, powder, sheets, putty, flowable fluid, etc.), or combinations of any two or more of such bone-derived matrices. Also provided are methods for making and using such multiple component grafts.

Abstract: Provided herein are compositions containing dehydrated placental tissue particulates, methods of making the compositions and methods for treating various musculoskeletal disorders and other conditions using such compositions, including osteoarthritis (OA), degenerative disc disease, tendonitis, plantar fasciitis, and pain associated therewith.

Abstract: A ligament-derived graft is provided which comprises a portion of a ligament sample recovered from a mammalian donor and which has been cut approximately in half lengthwise, where the ligament derived graft has dimensions suitable for use in a surgical procedure to repair small to mid-sized, or less load bearing joints. Small joints for which the ligament-derived grafts may be used to repair, include, interphalangeal, metacarpophalangeal joint, scapholunate joint, and other joints. In some embodiments, the dimensions of the ligament-derived graft include a length of from about 50 mm to about 150 mm, and a width of from about 3 mm to about 20 mm. In some embodiments, ligament samples used to make the ligament-derived grafts are lateral or medial collateral ligaments. Methods of using such ligament-derived grafts include attaching one or more ligaments, at their ends, to bones on opposite sides of the joint being repaired, so that the ligament(s) extend across the joint.

Abstract: Provided herein are compositions and methods for treating heart disease. In particular, provided herein are constructs, devices, and systems, each comprising one or more placenta-derived compositions, and their use in treating disorders involving aberrant cardiac rhythms and promoting repair of damaged cardiac tissue. The method of treatment comprises applying one or more constructs on the surface of all or a portion of the heart and/or adjacent tissues, during or after surgical heart treatment.

Abstract: Decellularized muscle matrices are provided for use as implants and grafts to repair, regenerate, supplement, reinforce and replace muscle tissue. The decellularized muscle matrices are derived from muscle tissue having preserved extracellular matrix components, retained muscle-forming potential, and from which immunogenic components have been removed. The decellularized muscle matrices are produced in various physical forms and combinations. Methods for making and using the decellularized muscle matrices are also provided.

Abstract: Provided herein are compositions containing dehydrated placental tissue particulates, methods of making the compositions and methods for treating various musculoskeletal disorders and other conditions using such compositions, including osteoarthritis (OA), degenerative disc disease, tendonitis, plantar fasciitis, and pain associated therewith.

Abstract: The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.

Abstract: In one embodiment, the present invention includes a method for repairing a cartilage defect including: preparing a cartilage defect by removing unwanted or damaged tissue; creating at least one perforation into or through subchondral bone, below and/or adjacent to the cartilage defect to induce the flow of bone fluid; allowing the bone fluid to bleed through the at least one perforation up into the cartilage defect to fill at least a portion of the cartilage defect; and applying a biomaterial into the defect to produce a clot for cartilage regeneration.

Abstract: In one embodiment, the present invention is a method of preparing a bioactive filamentary fixation device in situ, including (a) providing a filamentary fixation device including a sleeve member and a filament; (b) providing a physiological solution; (c) providing bioactive material; (d) wetting the sleeve of the fixation device with the physiological solution to produce a wetted sleeve; and (e) applying the bioactive material to the wetted sleeve to coat the sleeve, thus producing a bioactive filamentary fixation device at the time of surgery in situ.

Abstract: The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.

Abstract: Disclosed are methods of treating inflammation within a synovial joint, comprising administering to the joint a composition comprising primed stem or progenitor cells in an amount effective to enhance recovery, and a pharmaceutically acceptable carrier.

Abstract: In one embodiment, the present invention includes a method for repairing a cartilage defect including: preparing a cartilage defect by removing unwanted or damaged tissue; creating at least one perforation into or through subchondral bone, below and/or adjacent to the cartilage defect to induce the flow of bone fluid; allowing the bone fluid to bleed through the at least one perforation up into the cartilage defect to fill at least a portion of the cartilage defect; and applying a biomaterial into the defect to produce a clot for cartilage regeneration.

Abstract: Disclosed are methods of enhancing recovery from joint surgery, comprising administering to the joint during surgery a composition comprising stem or progenitor cells in an amount effective to enhance recovery, and a pharmaceutically acceptable carrier.

Abstract: Disclosed are methods of treating inflammation within a synovial joint, comprising administering to the joint a composition comprising primed stem or progenitor cells in an amount effective to enhance recovery, and a pharmaceutically acceptable carrier.

Abstract: The invention is directed to a method of accelerating healing in a joint comprising administering a platelet composition to the joint.

Abstract: The present invention includes a method for activating platelets in a platelet solution, the method may include passing the platelet solution through an activator at least once to activate at least one of the platelets in the platelet solution to produce an activated platelet solution. The activator may further include a flow disruption element. The method may further induce administering the activated platelet solution to the repair site of a patient. The present invention may also be a system for treating a medical condition at a repair site within a patient in need thereof, which may include a platelet solution container for housing a platelet solution; an activator to activate at least one platelet; and an introducer to direct the at least one activated platelet to the repair site.

Abstract: In one embodiment, the present invention may include a method of performing microdrilling surgery including directing a distal portion of a cannulated guide having an angle of curvature adjacent to a defect site; directing a drill, including a drill head and a flexible shaft, through the cannulated guide from a proximal portion of the guide through the distal portion and towards the defect site; drilling at least one hole into the defect site; and removing the drill and cannulated guide from the defect site and allowing blood or bone marrow to flow into the at least one hole towards the defect site.

Abstract: The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.

Abstract: The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.