Patents by Inventor Marek Kwiatkowski

Marek Kwiatkowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20040044195
    Abstract: Methods for deprotecting and derivatizing oligonucleotides that are non-covalently bound to a solid support are described.
    Type: Application
    Filed: August 28, 2002
    Publication date: March 4, 2004
    Inventor: Marek Kwiatkowski
  • Patent number: 6646118
    Abstract: The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
    Type: Grant
    Filed: March 25, 2002
    Date of Patent: November 11, 2003
    Assignee: Quiatech AB
    Inventors: Marek Kwiatkowski, Mats Nilsson, Ulf Landegren
  • Patent number: 6639088
    Abstract: A hydrocarbyldithiomethyl-modified compound of the Formula: R1—O—CH2—S—S—R2 or a salt thereof wherein R1 is an organic molecule and R2 is a hydrocarbyl is useful for protecting and/or blocking hydroxyl groups in organic molecules such as nucleotides. The hydrocarbyldithiomethyl-modified compounds can also be used for chemically synthesizing oligonucleotides and for sequencing nucleic acid compounds.
    Type: Grant
    Filed: September 12, 2001
    Date of Patent: October 28, 2003
    Assignee: Quiatech AB
    Inventor: Marek Kwiatkowski
  • Publication number: 20030153741
    Abstract: Methods for separating oligonucleotides are described. The oligonucleotides to be separated include separation tags attached to each end of the oligonucleotides.
    Type: Application
    Filed: February 8, 2002
    Publication date: August 14, 2003
    Inventor: Marek Kwiatkowski
  • Publication number: 20030003483
    Abstract: Synthetic probes and methods for detecting analytes using such probes are described. The probes contain an analyte reactive moiety linked to a nucleic acid molecule that can be extended by DNA polymerase.
    Type: Application
    Filed: May 9, 2002
    Publication date: January 2, 2003
    Inventor: Marek Kwiatkowski
  • Publication number: 20020177698
    Abstract: The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
    Type: Application
    Filed: March 25, 2002
    Publication date: November 28, 2002
    Applicant: Quiatech AB
    Inventors: Marek Kwiatkowski, Mats Nilsson, Ulf Landegren
  • Patent number: 6429309
    Abstract: The present invention relates to a support system for the solid phase synthesis of oligomers, such as oligonucleotide, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: August 6, 2002
    Assignee: Quiatech AB
    Inventors: Marek Kwiatkowski, Mats Nilsson, Ulf Landegren
  • Publication number: 20020051994
    Abstract: A method of preparing an immobilized oligonucleotide having a free 3′-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3′-end and having a free 5′-end; ii) binding said oligonucleotide in a second position remote from the 3′-end to the solid support; and iii) selectively making the 3′-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3′-end.
    Type: Application
    Filed: September 12, 2001
    Publication date: May 2, 2002
    Inventors: Marek Kwiatkowski, Ulf Landegren, Mats Nilsson
  • Publication number: 20020035247
    Abstract: The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
    Type: Application
    Filed: August 2, 2001
    Publication date: March 21, 2002
    Inventors: Marek Kwiatkowski, Mats Nilsson, Ulf Landegren
  • Publication number: 20020015961
    Abstract: A hydrocarbyldithiomethyl-modified compound of the Formula:
    Type: Application
    Filed: September 12, 2001
    Publication date: February 7, 2002
    Inventor: Marek Kwiatkowski
  • Patent number: 6313284
    Abstract: A method of preparing an immobilized oligonucleotide having a free 3′-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3′-end and having a free 5′-end; ii) binding said oligonucleotide in a second position remote from the 3′-end to the solid support; and iii) selectively releasing the 3′-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3′-end.
    Type: Grant
    Filed: November 12, 1999
    Date of Patent: November 6, 2001
    Inventors: Marek Kwiatkowski, Ulf Landegren, Mats Nilsson
  • Patent number: 6309836
    Abstract: A hydrocarbyldithiomethyl-modified compound of the Formula: R1—O—CH2—S—S—R2 or a salt thereof wherein R1 is an organic molecule and R2 is a hydrocarbyl is useful for protecting and/or blocking hydroxyl groups in organic molecules such as nucleotides. The hydrocarbyldithiomethyl-modified compounds can also be used for chemically synthesizing oligonucleotides and for sequencing nucleic acid compounds.
    Type: Grant
    Filed: October 5, 1999
    Date of Patent: October 30, 2001
    Inventor: Marek Kwiatkowski
  • Patent number: 6291669
    Abstract: The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage. Furthermore, the invention relates to a method for synthesis of oligonucleotides on a solid support. The support system comprises a stable disiloxyl linkage providing high nucleoside loadings to the support and the method allows convenient non-laborious oligomer synthesis.
    Type: Grant
    Filed: July 19, 1999
    Date of Patent: September 18, 2001
    Inventors: Marek Kwiatkowski, Mats Nilsson, Ulf Landegren
  • Patent number: 6255475
    Abstract: The invention relates to compounds of general structure (I) or salts thereof, wherein B is a nucleobase, X and Z independently are oxygen or sulphur, Y is hydrogen or hydroxy, which optionally may be protected, R1 is hydrocarbyl, which optionally is substituted with a functional group, R2 is hydrogen or hydrocarbyl, which optionally is substituted with a functional group, A is an electron withdrawing or electron donating group capable of moderating the acetal stability of compound (I), L1 and L2 are hydrocarbon linkers, which may be the same or different, L2, when present, being either (i) connected to L1 via the group A, or (ii) directly connected to L1, the group A then being connected to one of linkers L1 and L2, F is a dye label, Q is a coupling group for F, and l, m and n independently are 0 or 1, with the proviso that l is 1 when m is 1, and l is 1 and m is 1 when n is 1. The compounds of formula (I) are useful as deactivatable chain extension terminators.
    Type: Grant
    Filed: September 16, 1997
    Date of Patent: July 3, 2001
    Inventor: Marek Kwiatkowski
  • Patent number: 6235472
    Abstract: The present invention provides a nucleic acid detecting agent able to interact with a target sequence through catenation. The invention provides a nucleic acid detecting reagent comprising a) two free nucleic acid ends which are at least partially complementary to and capable of hybridizing to two adjacent regions of a target nucleic acid sequence; and b) an intermediate segment between the ends, the intermediate segment being labeled with a least one detectable element selected from the group consisting of chromophores, fluorophores, luminescers, radiolabels, affinity groups and enzymes, wherein only the ends of the reagent hybridize to the target nucleic acid sequence.
    Type: Grant
    Filed: June 18, 1998
    Date of Patent: May 22, 2001
    Inventors: Ulf Landegren, Marek Kwiatkowski
  • Patent number: 6127529
    Abstract: Bifuncitonal chelating pyridine compound and its use for conferring chelating properties on organic compounds. The pyridine compound has the structure ##STR1## where (i) n is an integer 1 or 2,(ii) R.sub.1, R.sub.2 and R.sub.3 represent groups that have no electrons capable of significantly delocalizing or resonating with the pyridine ring, such as hydrogen, alkyl or aralkyl having an aliphatic carbon atom next to the pyridine ring; at least two of R.sub.1, R.sub.2 and R.sub.
    Type: Grant
    Filed: September 13, 1991
    Date of Patent: October 3, 2000
    Assignee: Wallac Oy
    Inventors: Marek Kwiatkowski, Christian Sund, Jyrki Ylikoski, Veli-Matti Mukkala, Ilkka Hemmila
  • Patent number: 5871921
    Abstract: The invention relates to a method of detecting a target nucleic acid sequence in a sample by contacting the sample with a detectable probe having ends which hybridize to two adjacent regions of the target sequence. The hybridized probe ends are then covalently connected to form a cyclized structure interlocking with the target molecule. This structure is then subjected either to non-hybridizing conditions and/or to exonuclease activity to remove any non-cyclized probes from the target sequence. The target molecule is then detected by detecting the presence of the interlocking, cantenated probe.
    Type: Grant
    Filed: August 23, 1996
    Date of Patent: February 16, 1999
    Inventors: Ulf Landegren, Marek Kwiatkowski
  • Patent number: 5252462
    Abstract: The method for demonstrating the presence of an activity of an enzyme by(a) incubating said enzyme with a fluorogenic substrate A which is converted by the enzyme to a product B differing from A in respect to its fluorescent properties, A and/or B carrying a chromophore which is a triplet sensitizer having a triplet energy level above the excitation energy level of a lanthanide ion selected from the group consisting of Eu.sup.3+, Tb.sup.3+, Dy.sup.3+ and Sm.sup.3+ and which is capable of chelating said lanthanide ion by means of an oxygen or nitrogen atom in said chromophore, and that B differs from A either by(i) carrying a different chromophore, or(ii) having a different chelating ability, and(b) measuring the change in fluorescence caused by said enzyme.
    Type: Grant
    Filed: January 5, 1990
    Date of Patent: October 12, 1993
    Assignees: Pharmacia AB, Wallac Oy
    Inventors: Hakan Drevin, Anna T. Martin, Jan Carlsson, Sven O. Oscarsson, Timo Lovgren, Ilkka Hemmila, Marek Kwiatkowski
  • Patent number: 5216134
    Abstract: A chelate formed between Eu.sup.3+, Tb.sup.3+, Dy.sup.3+ and Sm.sup.3+ and a compound having the formula ##STR1## where A.sub.1-6 are single carbon or nitrogen atoms; n=1 or 2; R.sub.1-6 is nothing when A is a nitrogen, and hydrogen or an organic group when A is a carbon; Z and Z' are selected among --N(CH.sub.2 CH.sub.2 COO.sup.-).sub.2, --N(CH.sub.2 COO.sup.-).sub.2, --N(CH.sub.2 OPO.sub.3.sup.2-).sub.2 and --N(CH.sub.2 PO.sub.3.sup.2-).sub.2 ;--species that --X--Y is a substituent replacing a hydrogen anywhere in the parent compound; and --X--Y represents an organic group containing no chelating heteroatom closer than four atoms from a chelating heteroatom in the parent compound and X is a stable bridge containing certain groups and Y is selected from (a) specified relative groups allowing coupling to other compounds and (b) residues of compounds participating in biospecific affinity reactions.
    Type: Grant
    Filed: March 6, 1991
    Date of Patent: June 1, 1993
    Assignee: Wallac Oy
    Inventors: Veli-Matti Mukkala, Christian Sund, Marek Kwiatkowski