Patents by Inventor Mark A. Hayden

Mark A. Hayden has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230302453
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells.
    Type: Application
    Filed: April 24, 2023
    Publication date: September 28, 2023
    Applicant: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Peter J. Karabatsos, Andrew Fischer, John M. Robinson, Shelley R. Holets-McCormack, Sophie Laurenson
  • Patent number: 11697117
    Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.
    Type: Grant
    Filed: May 23, 2019
    Date of Patent: July 11, 2023
    Assignees: Abbott Laboratories, The Katholieke Universiteit Leuven
    Inventors: Mark A. Hayden, Karen Leirs, Elena Pérez-Ruiz, Jeroen Lammertyn
  • Patent number: 11633738
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: April 25, 2023
    Assignee: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Peter J. Karabatsos, Andrew Fischer, John M. Robinson, Shelley R. Holets-McCormack, Sophie Laurenson
  • Patent number: 11578358
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. The detection modules disclosed here can be used for detecting analytes of interest which analytes may have been enriched by amplification, isolation, or other techniques.
    Type: Grant
    Filed: September 14, 2017
    Date of Patent: February 14, 2023
    Inventors: Mark A. Hayden, Jeffrey B. Huff, Sophie Laurenson, Andrew Fischer, John Robinson, Shelley R. Holets-McCormack
  • Patent number: 11369963
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additional analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed.
    Type: Grant
    Filed: April 16, 2020
    Date of Patent: June 28, 2022
    Assignee: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Publication number: 20220126296
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additional analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed.
    Type: Application
    Filed: November 4, 2021
    Publication date: April 28, 2022
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Publication number: 20220088600
    Abstract: System for storing and dispensing liquid in a digital microfluidic chip includes a plurality of reservoir electrodes defining a reservoir having an outlet and a first end opposite the outlet, the reservoir configured to be in fluidic communication with at least one device electrode proximate the outlet, the at least one device electrode and at least one of the plurality of reservoir electrodes configured to generate electrical actuation forces to dispense at least one droplet from the reservoir through the outlet. The plurality of reservoir electrodes include a first reservoir electrode proximate the first end, a reservoir outlet electrode proximate the outlet, and at least one intermediate reservoir electrode disposed between the first electrode and the reservoir outlet electrode.
    Type: Application
    Filed: December 2, 2021
    Publication date: March 24, 2022
    Applicant: ABBOTT LABORATORIES
    Inventors: Jeffrey B. HUFF, Mark A. HAYDEN, Nicholas John COLLIER, Stephen BROWN, Karen Xin Zhou YU
  • Publication number: 20220091146
    Abstract: Digital microfluidic device includes a first substrate and a second substrate aligned generally parallel to each other with a gap defined therebetween in side view. At least one of the first substrate and the second substrate include a first electrode array, a second electrode array spaced from and in electrical communication with the first electrode array, and a first interstitial area defined between the first electrode array and the second electrode array. At least one of the first electrode array and the second electrode array is configured to generate electrical actuation forces within an actuation area to urge at least one droplet within the gap along the at least one of the first substrate and the second substrate. At least one spacer is disposed in the first interstitial area to maintain the gap between the first substrate and the second substrate.
    Type: Application
    Filed: December 2, 2021
    Publication date: March 24, 2022
    Applicant: ABBOTT LABORATORIES
    Inventors: Jeffrey B. HUFF, Mark A. HAYDEN, Nicholas John COLLIER, Stephen BROWN, Karen Xin Zhou YU
  • Publication number: 20220088599
    Abstract: Digital microfluidic and analyte detection device includes a first substrate and a second substrate aligned generally parallel to each other with a gap defined therebetween in side view. At least one of the first and second substrates has an electrode array configured to generate electrical actuation forces to urge at least one droplet within the gap along the at least one of the first substrate and the second substrate. The electrode array has a plurality of electrodes defining an electrode array area in plan view. At least one of the first substrate and the second substrate has a well array defining a well array area in plan view. The well array area is bounded within the electrode array area and overlapping a portion of each of the plurality of electrodes. The well array area overlaps less than 75% of the electrode array area in plan view.
    Type: Application
    Filed: December 2, 2021
    Publication date: March 24, 2022
    Applicant: ABBOTT LABORATORIES
    Inventors: Mark A. HAYDEN, Jeffrey B. HUFF, Nicholas John COLLIER, Karen Xin Zhou YU
  • Patent number: 11198129
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additonal analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed. The analyte detection chip may include a digital microfluidics (DMF) region and an analyte detection region which may overlap or may be spatially separated.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: December 14, 2021
    Assignee: Abbott Laboratories
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Publication number: 20210325334
    Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region.
    Type: Application
    Filed: April 2, 2021
    Publication date: October 21, 2021
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Patent number: 11022598
    Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: June 1, 2021
    Assignee: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Peter J. Karabatsos, Andrew S. Schapals, Anthony S. Muerhoff, M. Felicia Bogdan, Thomas Leary, Shelley R. Holets-McCormack, Sophie Laurenson, Andrew T. Fischer, Richard Haack, Stefan Hershberger, Dustin House, Lei Qiao, M. Shawn Murphy, Mark R. Pope, Edna M. Prieto-Ballengee, QiaoQiao Ruan, Pathik Soni, Sergey Tetin, Lyle Yarnell
  • Patent number: 11016053
    Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: May 25, 2021
    Assignee: Abbott Laboratories
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Publication number: 20200384470
    Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additional analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed.
    Type: Application
    Filed: April 16, 2020
    Publication date: December 10, 2020
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Graham Davis, Sergey Gershtein
  • Publication number: 20200256843
    Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
    Type: Application
    Filed: May 1, 2020
    Publication date: August 13, 2020
    Applicant: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Peter J. Karabatsos, Andrew S. Schapals, Anthony S. Muerhoff, M. Felicia Bogdan, Thomas Leary, Shelley R. Holets-McCormack, Sophie Laurenson, Andrew T. Fischer, Richard Haack, Stefan Hershberger, Dustin House, Lei Qiao, M. Shawn Murphy, Mark R. Pope, Edna M. Prieto-Ballengee, QiaoQiao Ruan, Pathik Soni, Sergey Tetin, Lyle Yarnell
  • Patent number: 10697009
    Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.
    Type: Grant
    Filed: August 12, 2019
    Date of Patent: June 30, 2020
    Assignee: IBIS BIOSCIENCES INC.
    Inventors: Mark A. Hayden, Jeffrey Huff, Mark W. Eshoo, John Picuri
  • Publication number: 20200123605
    Abstract: Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).
    Type: Application
    Filed: December 23, 2019
    Publication date: April 23, 2020
    Inventors: Mark W. Eshoo, John M. Clemens, Mark A. Hayden
  • Patent number: 10550428
    Abstract: Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: February 4, 2020
    Assignee: IBIS BIOSCIENCES, INC.
    Inventors: Mark W. Eshoo, John M. Clemens, Mark A. Hayden
  • Publication number: 20200001300
    Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.
    Type: Application
    Filed: May 23, 2019
    Publication date: January 2, 2020
    Inventors: Mark A. Hayden, Karen Leirs, Elena Pérez-Ruiz, Jeroen Lammertyn
  • Publication number: 20190367976
    Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.
    Type: Application
    Filed: August 12, 2019
    Publication date: December 5, 2019
    Inventors: Mark A. Hayden, Jeffrey Huff, Mark W. Eshoo, John Picuri