Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region.

Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additional analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed.

Abstract: Disclosed is a system for monitoring facial presentation of a first user to a second user. The system may include at least one image capturing device configured for capturing at least one image of the first user. Further, the system may include a communication device communicatively coupled to the at least one image capturing device. Further, the system may include a processing device communicatively coupled to the communication device. The communication device may be configured for transmitting the at least one image to a processing device. The processing device may be configured for analyzing the at least one image. The processing device may be configured for identifying a face of the first user based on the analyzing. Further, the processing device may be configured for generating facial presentation data based on the identifying. Further, the system may include a storage device configured for storing the facial presentation data.

Abstract: A tankless electric water heater system including a heating chamber having an inlet at a first end and an outlet at a second end, a heating element connected to the heating chamber, a first temperature sensor disposed near the first end of the heating chamber, a second temperature sensor disposed near the second end of the heating chamber, a flow sensor configured to detect a flow of water and disposed near the heating chamber, and a controller connected to the first and second temperature sensors, the flow sensor, and the heating element. The controller is configured to have a set point temperature, to detect temperature and flow data from the first and second temperature sensors, and the flow sensor, and to provide as output a power setting to the heating element.

Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Abstract: A liquid flow device to receive fluid and laminarize the fluid flow. The liquid flow device includes a jet nozzle assembly to receive the fluid and direct the fluid through a plurality of jet channels and a flow disruptor assembly to receive the fluid from the jet nozzle assembly to generate a flow path of the fluid. The liquid flow device also includes a chamber assembly connected to the flow disruptor to receive the fluid from the flow disruptor assembly and an outlet assembly, open to an external environment to receive the fluid from the chamber assembly and output the fluid with a laminar flow pattern.

Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.

Abstract: A tankless electric water heater system including a heating chamber having an inlet at a first end and an outlet at a second end, a heating element connected to the heating chamber, a first temperature sensor disposed near the first end of the heating chamber, a second temperature sensor disposed near the second end of the heating chamber, a flow sensor configured to detect a flow of water and disposed near the heating chamber, and a controller connected to the first and second temperature sensors, the flow sensor, and the heating element. The controller is configured to have a set point temperature, to detect temperature and flow data from the first and second temperature sensors, and the flow sensor, and to provide as output a power setting to the heating element.

Abstract: Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).

Abstract: Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).

Abstract: The present invention is a system to utilize slow burning fuels that contain ash with a piston that is utilized as a compressor and an expander, a pair of combustion chambers, an ash bin with a pathway on each side of the ash bin and a cylinder that houses the piston that is integral to the y-shaped pathway and produces gases that are produced by the piston emitted from the cylinder as well as a connecting rod and a crank shaft. The combustion chambers can be cone-shaped and have one or more fan blades that are made of a nonconductive material and have a positive charge. The present invention also creates a rotating body in a single combustion chamber that provides heat energy for evaporation of water and initiation of ignition of fuels that contain more than 5% water that can be connected to an ash bin to expel ash.

Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.

Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.

Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.

Abstract: Methods for detecting target analytes utilizing an array of wells are advantageous for detection of low concentrations of target analytes. Use of an array of wells requires sealing of the wells. The methods provided herein utilize digital microfluidics to seal wells of an array with a fluid that is immiscible with the aqueous liquid present in the wells to prevent evaporation and contamination of the aqueous fluid during analysis of signals from the wells. The disclosed method include generating a biphasic droplet composed of the immiscible fluid and an aqueous fluid. The immiscible fluid present in the biphasic droplet is moved over the array of wells to seal the wells by electrically actuating the aqueous fluid present in the biphasic droplet which in turn pulls the immiscible fluid.

Abstract: Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. The detection modules disclosed here can be used for detecting analytes of interest which analytes may have been enriched by amplification, isolation, or other techniques.

Abstract: The present invention provides systems, methods, and compositions for performing molecular tests. In particular, the present invention provides methods, compositions and systems for generating target sequence-linked solid supports (e.g., beads) using a solid support linked to a plurality of capture sequences and capture primers composed of a 3? target-specific portion and a 5? capture sequence portion. In certain embodiments, the target sequence linked solid support is used in sequencing methods (e.g., pyrosequencing, zero-mode waveguide type sequencing, nanopore sequencing, etc.) to determine the sequence of the target sequence (e.g., in order to detect the identity of a target nucleic acid in sample).

Abstract: A liquid flow device to receive fluid and laminarize the fluid flow. The liquid flow device includes a jet nozzle assembly to receive the fluid and direct the fluid through a plurality of jet channels and a flow disruptor assembly to receive the fluid from the jet nozzle assembly to generate a flow path of the fluid. The liquid flow device also includes a chamber assembly connected to the flow disruptor to receive the fluid from the flow disruptor assembly and an outlet assembly, open to an external environment to receive the fluid from the chamber assembly and output the fluid with a laminar flow pattern.

Abstract: Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein:nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide.