Abstract: The present invention provides for separation of bacterial species and serotypes using electrophoretic methods.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Abstract: In some embodiments, a crossbow crank comprises a housing and a shaft rotatable with respect to the housing. A one-way mechanism is arranged to prevent rotation of the shaft in a first rotational direction, but allow rotation in a second direction. A release mechanism is arranged to disengage the one-way mechanism from the shaft. The release mechanism has a first position and a second position, wherein the release mechanism moves along a length of the shaft between the first position and the second position.

Abstract: In some embodiments, a crossbow crank comprises a housing and a shaft rotatable with respect to the housing. A one-way mechanism is arranged to prevent rotation of the shaft in a first rotational direction, but allow rotation in a second direction. A release mechanism is arranged to disengage the one-way mechanism from the shaft. The release mechanism has a first position and a second position, wherein the release mechanism moves along a length of the shaft between the first position and the second position.

Abstract: The present invention provides for separation of bacterial species and serotypes using electrophoretic methods.

Abstract: A mixing system includes a housing having a motor mount rotatably coupled thereto, the motor mount having a passage extending therethrough. A drive shaft is removably positioned within the passage of the motor mount. A cap includes a main body removably coupled to the motor mount and an actuator coupled to the main body so as to be pivotable between a first position and a second position with respect to the main body. The actuator producing a camming action when the actuator is pivoted such that when the actuator is in the first position, the actuator pushes the drive shaft against the motor mount so that the main body is locked to the motor mount and so that rotation of the motor mount causes rotation of the drive shaft and when the actuator is in the second position, the actuator is disengaged from the drive shaft and the cap is removable from the motor mount.

Abstract: The present invention provides devices and methods to separate and concentrate target species. In some embodiments, a punctuated continuous microchannel or parallel processing (array-based) separations are provided, the microchannel having a plurality of sequential, constrictive insulating features to form a plurality of reservoirs including a first, second and third reservoir, and a plurality of constricted passageways including a first constricted passageway that connects the first reservoir to the second reservoir and a second constricted passageway that connects the second reservoir to the third reservoir. A voltage is applied to the microchannel to create different electrical fields and/or different dielectrophoresis (DEP) gradients at each of the plurality of constricted passageways in order to separate species that have differing ratios of electrokinetic mobility to dielectrophoretic mobility.

Abstract: The present invention provides for separation of bacterial species and serotypes using electrophoretic methods.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Abstract: The present invention provides devices and methods to separate and concentrate target protein species at a microliter scale and to generate reagents to those variants with exquisite selectivity for specific protein isoforms using only picograms of target material.

Abstract: In some embodiments, a crossbow crank comprises a housing and a shaft rotatable with respect to the housing. A one-way mechanism is arranged to prevent rotation of the shaft in a first rotational direction, but allow rotation in a second direction. A release mechanism is arranged to disengage the one-way mechanism from the shaft. The release mechanism has a first position and a second position, wherein the release mechanism moves along a length of the shaft between the first position and the second position.

Abstract: Disclosed are embodiments of methods, apparatus, systems, compositions, and articles of manufacture relating to identifying the source of bioparticles, such as bioparticles shed by an organism. In embodiments, a method may include collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most informative for identifying their source, and gathering data from those bioparticles to form bioparticle signatures; the bioparticle signatures may be processed into a multi-dimensional vector which may be compared to a multi-dimensional vector derived from a standard using a pattern recognition strategy. In embodiments, an apparatus may include a particle collection device to collect a sample, a transfer device to select bioparticles, and a detector that restricts the movement of the bioparticles; the restricted movement may be used to produce a bioparticle signature.

Abstract: The invention relates to a method for identifying the source of shed bioparticles and an apparatus that implements the method. The method involves collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most effective in identifying their source, and gathering data from those bioparticles to form bioparticle signatures. The bioparticle signatures are then processed into a multi-dimensional vector which is then compared to the multi-dimensional vector derived from a standard using a pattern recognition strategy that identifies the source. The apparatus has a particle collection device to collect the sample, a transfer device that selects information-rich bioparticles and a detector that restricts the movement of the information-rich bioparticles. The restricted movement is then translated into a bioparticle signature.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Abstract: Data objects are replicated from a source storage managed by a source server to a target storage managed by a target server. A source list is built of objects at the source server to replicate to the target server. The target server is queried to obtain a target list of objects at the target server. A replication list is built indicating objects on the source list not included on the target list to transfer to the target server. For each object in the replication list, data for the object not already at the target storage is sent to the target server and metadata on the object is sent to the target server to cause the target server to include the metadata in an entry for the object in a target server replication database. An entry for the object is added to a source server replication database.

Abstract: Data objects are replicated from a source storage managed by a source server to a target storage managed by a target server. A source list is built of objects at the source server to replicate to the target server. The target server is queried to obtain a target list of objects at the target server. A replication list is built indicating objects on the source list not included on the target list to transfer to the target server. For each object in the replication list, data for the object not already at the target storage is sent to the target server and metadata on the object is sent to the target server to cause the target server to include the metadata in an entry for the object in a target server replication database. An entry for the object is added to a source server replication database.

Abstract: A process is disclosed by which data is securely deleted in a transactionally consistent manner. This may be accomplished by committing a preparation transaction for a data object within a system managing the data object in order to return the system to an initial condition if necessary, attempting to commit an execution transaction with the data object only after committing the preparation transaction, and securely deleting any portion of the data object necessary to return the system to the initial condition if committing the execution transaction fails and to change the system to a completed condition only if committing the execution transaction succeeds. In a delete or move transaction an existing backup object may be assigned a new logically deleted state such that if the delete or move transaction fails, the data will be made accessible again.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.