Patents by Inventor Mark Isalan

Mark Isalan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190247519
    Abstract: Disclosed herein are novel nucleic acid molecules which encode novel polypeptides for use in treating polyglutamine diseases such as Huntington's disease. The polypeptides of the invention exhibit reduced immunotoxity in vivo and may have utility in sustained (mid- or long-term) expression in vivo. Also disclosed are methods for the treatment of polyglutamine diseases, such as Huntington's disease and the use of nucleic acids and polypeptides of the invention in medical therapy. Gene therapy methods and compositions, such as AAV vectors for use in gene therapy methods are also disclosed.
    Type: Application
    Filed: November 4, 2016
    Publication date: August 15, 2019
    Applicants: IMPERIAL INNOVATIONS LIMITED, FUNDACIÓ CENTRE DE REGULACIÓ GENÒMICA
    Inventor: Mark ISALAN
  • Patent number: 9732129
    Abstract: Disclosed herein are zinc finger peptides having at least 4 zinc finger domains, such as 6, 11, 12 or 18 zinc finger domains. The zinc finger peptides may be fused to effector domains for modulating gene expression. Zinc finger peptides of the invention are useful for targeting trinucleotide-repeat sequences in mutant genes and may have applications in gene therapy. The zinc finger peptides may have nucleic acid recognition sequences according to SEQ ID NO: 101. Also disclosed are methods for constructing poly-zinc finger peptides having arrays of at least 8 zinc finger domains, along with zinc finger frameworks that may be useful for selecting zinc finger peptides from libraries.
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: August 15, 2017
    Assignee: Fundacio Centre de Regulacio Genomica
    Inventors: Mark Isalan, Mireia Garriga-Canut
  • Publication number: 20160200781
    Abstract: Disclosed herein are zinc finger peptides having at least 4 zinc finger domains, such as 6, 11, 12 or 18 zinc finger domains. The zinc finger peptides may be fused to effector domains for modulating gene expression. Zinc finger peptides of the invention are useful for targeting trinucleotide-repeat sequences in mutant genes and may have applications in gene therapy. The zinc finger peptides may have nucleic acid recognition sequences according to SEQ ID NO: 101. Also disclosed are methods for constructing poly-zinc finger peptides having arrays of at least 8 zinc finger domains, along with zinc finger frameworks that may be useful for selecting zinc finger peptides from libraries.
    Type: Application
    Filed: May 18, 2015
    Publication date: July 14, 2016
    Applicant: FUNDACIO PRIVADA CENTRE DE REGULACIO GENOMICA
    Inventors: Mark Isalan, Mireia Garriga-Canut
  • Patent number: 9096682
    Abstract: Disclosed herein are zinc finger peptides having at least 4 zinc finger domains, such as 6, 11, 12 or 18 zinc finger domains. The zinc finger peptides may be fused to effector domains for modulating gene expression. Zinc finger peptides of the invention are useful for targeting trinucleotide-repeat sequences in mutant genes and may have applications in gene therapy. The zinc finger peptides may have nucleic acid recognition sequences according to SEQ ID NO: 101. Also disclosed are methods for constructing poly-zinc finger peptides having arrays of at least 8 zinc finger domains, along with zinc finger frameworks that may be useful for selecting zinc finger peptides from libraries.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: August 4, 2015
    Assignee: FUNDACIO PRIVADA CENTRE DE REGULACIO GENOMICA
    Inventors: Mark Isalan, Mireia Garriga-Canut
  • Patent number: 8618024
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Grant
    Filed: August 31, 2006
    Date of Patent: December 31, 2013
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Patent number: 8617807
    Abstract: The invention provides a method for designing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn; if base 4 of the quadruplet is G, the position +6 in the alpha helix is Arg or Lys; if position 4 in the quadruplet is T, then position +6 in the alpha helix is Ser, Thr, Val or Lys.
    Type: Grant
    Filed: July 14, 2006
    Date of Patent: December 31, 2013
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20130336947
    Abstract: Disclosed herein are zinc finger peptides having at least 4 zinc finger domains, such as 6, 11, 12 or 18 zinc finger domains. The zinc finger peptides may be fused to effector domains for modulating gene expression. Zinc finger peptides of the invention are useful for targeting trinucleotide-repeat sequences in mutant genes and may have applications in gene therapy. The zinc finger peptides may have nucleic acid recognition sequences according to SEQ ID NO: 101. Also disclosed are methods for constructing poly-zinc finger peptides having arrays of at least 8 zinc finger domains, along with zinc finger frameworks that may be useful for selecting zinc finger peptides from libraries.
    Type: Application
    Filed: October 17, 2011
    Publication date: December 19, 2013
    Applicant: FUNDACIÓ PRIVADA CENTRE DE REGULACIÓ GENÓMICA
    Inventors: Mark Isalan, Mireia Garriga-Canut
  • Patent number: 7947469
    Abstract: Disclosed herein are methods and compositions for modulating HIV infection by regulating expression of one or more receptors.
    Type: Grant
    Filed: January 22, 2002
    Date of Patent: May 24, 2011
    Assignee: Gendaq, Ltd.
    Inventors: Michael Moore, Mark Isalan, Lindsey Reynolds, Christopher Ullman, John Girdlestone, Christophe Demaison, Yen Choo
  • Patent number: 7759059
    Abstract: The application provides methods of designing zinc finger binding polypeptides for binding to particular target sequences comprising overlapping nucleotide quadruplets.
    Type: Grant
    Filed: August 31, 2006
    Date of Patent: July 20, 2010
    Assignee: Gendaq Limited, c/o Brobeck Hale and Dorr
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20100167357
    Abstract: An obligate heterodimer meganuclease consisting of a first and a second monomer, deriving from two different homodimeric LAGLIDADG (SEQ ID NO: 66) endonuclease monomers (parent monomers), and having at least one pair of mutations interesting corresponding residues of said parent monomers which make an intermolecular interaction between the two monomers of each parent homodimeric LAGLIDADG (SEQ ID NO: 66) endonuclease, a vector encoding said meganuclease, a cell, an animal or a plant modified by said vector and the use of said meganuclease and derived products for molecular biology, genome engineering and genome therapy.
    Type: Application
    Filed: January 31, 2008
    Publication date: July 1, 2010
    Applicant: CELLECTIS
    Inventors: Emmanuel Fajardo Sanchez, Sylvestre Grizot, Mark Isalan, Luis Serrand Pubul, Francois Stricher
  • Patent number: 7700523
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Grant
    Filed: September 1, 2006
    Date of Patent: April 20, 2010
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20070161014
    Abstract: The application provides methods of designing zinc finger binding polypeptides for binding to particular target sequences comprising overlapping nucleotide quadruplets.
    Type: Application
    Filed: August 31, 2006
    Publication date: July 12, 2007
    Applicant: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Patent number: 7241573
    Abstract: Disclosed herein are methods for designing DNA binding proteins comprising a plurality of zinc fingers and methods for binding the proteins to target nucleotide sequences in cells.
    Type: Grant
    Filed: April 26, 2004
    Date of Patent: July 10, 2007
    Assignee: Gendaq Ltd.
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Patent number: 7241574
    Abstract: Disclosed herein are methods for designing DNA binding proteins comprising a plurality of zinc fingers and methods for binding the proteins to target nucleotide sequences in cells.
    Type: Grant
    Filed: May 24, 2004
    Date of Patent: July 10, 2007
    Assignee: Gendaq Ltd.
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20070077227
    Abstract: The invention provides a method for designing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn; if base 4 of the quadruplet is G, the position +6 in the alpha helix is Arg or Lys; if position 4 in the quadruplet is T, then position +6 in the alpha helix is Ser, Thr, Val or Lys.
    Type: Application
    Filed: July 14, 2006
    Publication date: April 5, 2007
    Applicant: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20070009962
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Application
    Filed: August 31, 2006
    Publication date: January 11, 2007
    Applicant: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20070009948
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Application
    Filed: September 1, 2006
    Publication date: January 11, 2007
    Applicant: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Publication number: 20060166864
    Abstract: Disclosed herein are engineered zinc finger proteins which bind to telomeric structures; methods for their design, synthesis and use; and cells comprising a telomere-binding zinc finger protein. Methods for modulating telomerase activity using telomere-binding zinc finger proteins are also provided.
    Type: Application
    Filed: April 4, 2006
    Publication date: July 27, 2006
    Inventors: Yen Choo, Mark Isalan, Sachin Patel, Shankar Balasubramanian, Xiohai Liu
  • Patent number: 6977154
    Abstract: The invention provides a method for producing a zinc finger polypeptide which binds to a target nucleic acid sequence containing a modified base but not to an identical sequence containing an equivalent unmodified base.
    Type: Grant
    Filed: March 17, 1999
    Date of Patent: December 20, 2005
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Mark Isalan
  • Patent number: 6866997
    Abstract: Disclosed herein are methods of designing zinc finger binding polypeptides for binding to particular target sequences comprising overlapping nucleotide quadruplets.
    Type: Grant
    Filed: May 26, 1998
    Date of Patent: March 15, 2005
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan