Abstract: A stopper rod comprises a body (11) having a central passageway (12) with part of a carrier rod (13) fixed in the passageway and a further part outside of the passageway for attachment to a lifting device. An enlarged part of the passageway defines a sealing surface (14) and an insert (16) around the carrier rod is partly received in the enlarged part. Retention means (17, 18) are carried on the carrier rod and expandable means are disposed around the carrier rod between the insert (16) and the retention means (17, 18). The expandable means include an expandable graphitic composition formed by removing a proportion of interstitial water from an expandable graphite formulation. The insert has a co-efficient of thermal expansion no greater than that of the carrier rod, whilst expansion of the expandable means is greater than that of the carrier rod.

Abstract: Embodiments provide unique and novel systems and methods for deleting data on removable disk drives. In embodiments, the removable disk drives store data that may be erased such that the data is considered destroyed but that allows the removable disk drive to be reused. The archiving system can determine which data should be erased. Then, the data is digitally shredded on sector boundaries of the removable disk drives such that the reclaimed memory cannot be read to decipher the erased data. In alternative embodiments, data is written across sector boundaries such that two or more files may occupy a single sector. A journal area, in embodiments, allows for copying the data in a sector with two or more files, digitally shredding the sectors in the removable disk drive, and rewriting the data that was not to be digitally shredded to the original location of the data.

Abstract: Embodiments provide systems and methods for maintaining immutable data in an archiving system using random access memory. To ensure data is immutable, novel pointers are maintained in the hardware/firmware of the drive ports and on the removable disk drives. For example, a hardware/firmware in a modular drive bay maintains a pointer to a memory address in the removable disk drive memory that cannot write to a memory block that precedes the pointer. Data may only be stored after the pointer in the removable disk drive. As such, once data is written to the removable disk drive, the data cannot be overwritten although the removable disk drive employs random access memory.

Abstract: A stopper rod for controlling the flow of molten steel from a tundish has a metal carrier element partly received in an internal passageway of the stopper rod, the carrier element compressing sealing means between a frustoconical undersurface of a laterally enlarged portion thereof and a complementary seating surface of the stopper rod passageway, there being an insert threadedly engaged with one of the carrier element and stopper rod body at a position above a laterally enlarged portion of the carrier element, with further sealing means disposed between said insert and the carrier element. Preferably the insert is a nut screwed down onto a washer which compresses the further sealing means.

Abstract: A brachytherapy treatment device includes at least one tubular insertion member, an expandable member, and means for deflecting the at least one tubular insertion member. The tubular insertion member has a longitudinal axis and proximal and distal ends. The expandable member is disposed on and surrounding the distal end of the tubular insertion member. The distal end of the at least one deflected tubular insertion member within the first expandable member is offset from the longitudinal axis when deflected. The at least one deflected tubular insertion member is configured to receive a radiation source to position a radiation source offset with regard to the longitudinal axis to form an asymmetric radiation dosing profile. Additional brachytherapy treatment devices and methods for forming an asymmetric radiation dosing profile are disclosed.

Abstract: A device for verifying occlusion of the fallopian tube in a female subject includes an elongate gas delivery member having a lumen disposed therein, the elongate gas delivery member adapted for sealing engagement with the subject's uterus. The device includes a pressurized insufflation gas source coupled to the elongate gas delivery member, the insufflation gas source being in communication with the lumen of the elongate gas delivery member. The insufflation gas may includes for example, carbon dioxide. In one aspect, the device includes a pressure sensor or gauge to measure intra-uterine pressure to verify occlusion of the fallopian tube(s). In another aspect, the flow rate of insufflation gas into the uterus may be measured using a flow meter to verify occlusion of the fallopian tube(s).

Abstract: A medical device and procedure is described for sterilizing a female. A two-part procedure includes an immediate sterilization part and a permanent sterilization part. The immediate sterilization part provides immediate sterility that endures at least until the permanent effects of a tubal occlusion procedure provide permanent sterility.

Abstract: A system and method of operating a storage system is provided. The storage system includes a tape cartridge having a magnetic tape medium and a tape drive for reading and writing data to the magnetic tape medium of the tape cartridge. Performance data associated with specific operating loci (e.g., bands, directions, tracks, segments, etc.) of the tape drive and tape medium are collected, analyzed, and stored, allowing for analysis and detection of trends over time. In one example, tape usage events are monitored and when they cross a threshold the system may remove particular tapes in a preventative maintenance approach to reduce system outage. Performance data is collected and analyzed with a portion of the performance data being saved to emulated medium auxiliary memory (EMAM) on the magnetic tape, and a portion of the of the performance data saved in non-volatile storage in the drive.

Abstract: A pivoting mid-floor section for use in a feeder house in an agricultural combine is located between the transverse drum and the transverse sprocket means. The pivoting mid-floor can move or adjust when a large volume of crop material is fed into the feeder house, while providing support for the conveyor chain along substantially the entire length of travel between the transverse drum and sprocket means, thus reducing the friction on the chain, and preventing the system slow downs that occurred in feeder house systems using a fixed mid-floor. Additionally, replaceable wear-resistant surfaces can be affixed to the runners or guides of the mid-floor to further extend wear life and reduce friction between the chain slats and mid-floor. The friction occurs between the wear pads on the runners or guides and the chain slats, rather than between the chain components and the mid-floor.

Abstract: Various methods are provided for the enzymatic production of glycolic acid from glycolonitrile. These methods include: 1) use of Acidovorax facilis 72W nitrilase mutants having improved nitrilase activity for converting glycolonitrile to glycolic acid, and 2) methods to improve catalyst stability and/or productivity. The methods to improve catalyst stability/productivity include use of reaction stabilizers, running the reactions under substantially oxygen free conditions, and controlling the concentration of substrate in the reaction mixture.

Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitriles to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidovorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.

Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.

Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitrites to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted in situ with an inorganic peroxide in the presence of a non-heme haloperoxidase having perhydrolysis activity to produce peroxycarboxylic acids.

Abstract: A new and improved conveyor feeder house chain slat having a unique shape and structure that provides sufficient structural rigidity to improve crop throughput while reducing clogging of crop material. The unique slat also provides additional support to the chain. Because the chain rides on the rear of the elongated section of the slat, the slat contacts the feed house, rather than the chain, reducing chain wear, overheating and lubricant evacuation. Because of the new slat configuration, the slats can be secured to the chain with removable securing means, enabling easier replacement of an individual slat if necessary.

Abstract: The invention relates to the isolation, sequencing, and recombinant expression of genes encoding either a nitrile hydratase (NHase) or amidase (Am) from Comamonas testosteroni 5-MGAM-4D, where the NHase is useful for catalyzing the hydration of nitrites to the corresponding amides, and the amidase is useful for hydrolysis of amides to the corresponding carboxylic acids. Also provided are transformed host cells containing polynucleotides for expressing the nitrile hydratase or amidase enzymes from Comamonas testosteroni 5-MGAM-4D.

Abstract: This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Abstract: A process is provided to produce a concentrated aqueous peracid solution in situ using at least one enzyme having perhydrolase activity in the presence of hydrogen peroxide (at a concentration of at least 500 mM) under neutral to acidic reaction conditions from suitable carboxylic acid esters (including glycerides) and/or amides substrates. The concentrated peracid solution produced is sufficient for use in a variety of disinfection and/or bleaching applications.

Abstract: A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.