Abstract: The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.

Abstract: A method and apparatus for setting cookies on client devices 6, in particular for allowing maintenance of cross domain session continuity. A server 5 is provided for extracting a session ID from a first party cookie in the domain of the server 5 which is included in a HTTP request generated by a page 8 from a different domain active in a browser 7. Code is returned by the server 5 to that page 8 to cause it to generate a first party cookie in its domain which includes the extracted session ID.

Abstract: A method and apparatus for setting cookies on client devices 6, in particular for allowing maintenance of cross domain session continuity. A server 5 is provided for extracting a session ID from a first party cookie in the domain of the server 5 which is included in a HTTP request generated by a page 8 from a different domain active in a browser 7. Code is returned by the server 5 to that page 8 to cause it to generate a first party cookie in its domain which includes the extracted session ID.