Patents by Inventor Martin A. Putnam

Martin A. Putnam has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10414143
    Abstract: A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: September 17, 2019
    Assignee: CyVek, Inc.
    Inventor: Martin A. Putnam
  • Patent number: 10252263
    Abstract: Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/?25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: April 9, 2019
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 10220385
    Abstract: A method for preparing a plurality of micro-length tubular flow elements for use in a fluid assay, each element having interior and exterior surfaces and having at least one axially-extending flow passage through its interior, includes aggressively agitating the flow elements in a solution of assay capture agent to impart disrupting shear forces on the exterior surface of the elements, the shear forces causing the axially-extending external surfaces of the flow elements to be free of assay capture agent, while at least a portion of the interior surface of the flow elements not experiencing such disruptive shear forces and carrying deposits of the assay capture agent.
    Type: Grant
    Filed: November 1, 2016
    Date of Patent: March 5, 2019
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood, Jane M. Tu
  • Publication number: 20190061329
    Abstract: A method of making at least a portion of at least one microfluidic actuator having a flexible diaphragm portion and an opposite surface portion, the diaphragm and opposite surface each having opposed faces, at least one of the faces comprising surface-activated PDMS, and the opposed faces being arranged such that when the opposed faces contact each other, they form a fluidic seal, including performing repeated make-and-break-contact protocol on the contacting opposed faces until the tendency for permanent bonds to form between the contacting faces has been neutralized, thereby enabling the diaphragm portion to perform actuated movements to engage and disengage with the opposite surface portion, without the diaphragm sticking to the opposite surface portion.
    Type: Application
    Filed: August 31, 2018
    Publication date: February 28, 2019
    Inventor: Martin A. Putnam
  • Patent number: 10209250
    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 19, 2019
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 10076752
    Abstract: A method of flowing a fluid with a tracer in a microfluidic channel of an assay device and detecting the tracer for determining the channel location or condition of the channel.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: September 18, 2018
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 10065403
    Abstract: In assembling a portion of a microfluidic device by conducting bonding action by contacting faces of opposed bondable materials, one comprising a flexible sheet, the method, while maintaining continual contact of the faces in a region R2 until bonding is completed, of employing repeated make-and-break-contact manufacturing protocol on a region R1 of the contacted faces of the bondable materials, thereby over time neutralizing the tendency for permanent bonds to form in the region R1, thus to enable making and breaking actuated movements of the region R1 of the flexible sheet relative to the portion of the other material that it opposes.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: September 4, 2018
    Assignee: CyVek, Inc.
    Inventor: Martin A. Putnam
  • Patent number: 10022696
    Abstract: A method of making an assay device comprising providing micro-elements in the form of micro-particles or micro-length tube detection elements and thereafter with an automated tool, picking and placing the micro-elements into open-sided microfluidic channels in a body.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: July 17, 2018
    Assignee: Cyvek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9855735
    Abstract: A method of forming a pneumatically controlled microfluidic device containing a micro-fluidic network that includes micro-elements in the form of micro-particles or micro-length hollow flow elements, and a pneumatic network that includes pneumatic micro-channels and micro-valve features enabling membrane valves to operate to control fluid conditions in the microfluidic network, including the steps (a) forming two portions, denominated fluidic layer, in which microfluidic channels are formed, and pneumatic layer, in which pneumatic micro-channels and micro-valve features are formed, each having a backing that is rigid in the plane of extent of the layers, (b) providing an intervening elastic membrane, and (c) permanently bonding both layers to opposite sides of the membrane, the permanent bonding of the membrane to the fluidic layer being effective to permanently enclose a set of inserted micro-elements in the fluidic network and relate the two layers to enable pneumatic control of fluid conditions in the micr
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: January 2, 2018
    Assignee: CyVek, Inc.
    Inventor: Martin A. Putnam
  • Publication number: 20170307601
    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.
    Type: Application
    Filed: June 30, 2017
    Publication date: October 26, 2017
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20170297022
    Abstract: A method of flowing a fluid with a tracer in a microfluidic channel of an assay device and detecting the tracer for determining the channel location or condition of the channel.
    Type: Application
    Filed: April 28, 2017
    Publication date: October 19, 2017
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9759718
    Abstract: A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: September 12, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20170248621
    Abstract: A method and system for heating and/or inspecting a portable microfluidic assay cartridge for performing an assay includes receiving the assay cartridge on a receiving region of a translatable table under automated control, heating the cartridge, during performance of the assay, with a planar radiant heater plate, the heater plate having an aperture through which an inspection axis extends, and/or inspecting the cartridge using an optical system constructed to inspect the cartridge along the inspection axis by reading a fluorescent light signal which passes through the aperture in the heater plate. In addition, the cartridge moves with movement of the translation table, and the heater plate and optical system may be stationary, and the inspection axis may be fixed.
    Type: Application
    Filed: April 3, 2017
    Publication date: August 31, 2017
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9700889
    Abstract: A method of flowing a fluid with a tracer in a microfluidic channel of an assay device and detecting the tracer for determining the channel location or condition of the channel.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: July 11, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9651568
    Abstract: An operating and reading instrument for performing an assay employing a portable microfluidic assay cartridge, the instrument comprising a translatable table under automated control, the translatable table carrying a receiving region for the portable cartridge and carrying a port system connectable to the cartridge that includes at least one remotely automated valve carried by the translatable table, the valve arranged to apply pressurized flowable substance at selected times to the cartridge while the cartridge is on the translatable table.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: May 16, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20170050186
    Abstract: A method for preparing a plurality of micro-length tubular flow elements for use in a fluid assay, each element having interior and exterior surfaces and having at least one axially-extending flow passage through its interior, includes aggressively agitating the flow elements in a solution of assay capture agent to impart disrupting shear forces on the exterior surface of the elements, the shear forces causing the axially-extending external surfaces of the flow elements to be free of assay capture agent, while at least a portion of the interior surface of the flow elements not experiencing such disruptive shear forces and carrying deposits of the assay capture agent.
    Type: Application
    Filed: November 1, 2016
    Publication date: February 23, 2017
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood, Jane M. Tu
  • Patent number: 9546932
    Abstract: A pneumatically driven portable assay cartridge having analyte capture regions associated with microfluidic channels within its interior, the portable cartridge having pneumatic ports clampable against pneumatic ports of an operating instrument for controlled application of positive pressure and vacuum to pneumatic operating channels within the cartridge, the cartridge having a well for receiving sample from a user and microfluidic channels that include pneumatically operated pistons and valves controllable by the pneumatic operating channels to cause all flows of the assay from the reservoirs through reaction regions within the cartridge to on-board waste reservoir during conduct of the assay.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: January 17, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9500645
    Abstract: A microfluidic assay device that defines a micro-fluidic flow channel (44) having a flow axis, in which a series of discrete, axially-spaced apart, transparent hollow flow elements (32) are secured in fixed position, each flow element having at least one axially-extending flow passage through its interior, assay capture agent fixed to the interior surface of the elements for capture of an analyte in liquid flowing through the interior of the flow elements, the device constructed to enable light to be transmitted out of the elements for reading of fluorescence from captured analyte, wherein: the exterior axially-extending surfaces of the flow elements are free of active capture agent, while at least part of the interior surfaces carry deposits of active capture agent exposed to flow through the elements.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: November 22, 2016
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood, Jane M. Tu
  • Patent number: 9477042
    Abstract: Techniques and systems suitable for performing low-loss fusion splicing of optical waveguide sections are provided. According to some embodiments, multiple laser beams (from one or more laser) may be utilized to uniformly heat a splice region including portions of the optical waveguide sections to be spliced, which may have different cross-sectional dimensions. According to some embodiments, the relative distance of the optical waveguide sections and/or the power of the multiple laser beams may be varied during splicing operations.
    Type: Grant
    Filed: December 6, 2011
    Date of Patent: October 25, 2016
    Assignee: Weatherford Technology Holdings, LLC
    Inventors: Mark R. Fernald, Trevor W. MacDougall, Martin A. Putnam, Rebecca S. Bryant, Christopher J. Wright, Michael Arcand, Christopher T. Chipman
  • Publication number: 20160158750
    Abstract: Microfluidic devices are provided for conducting fluid assays, for example biological assays, that have the ability to move fluids through multiple channels and pathways in a compact, efficient, and low cost manner. Discrete flow detection elements, preferably extremely short hollow flow elements, with length preferably less than 700 micron, preferably less than 500 micron, and internal diameter preferably of between about 50+/?25 micron, are provided with capture agent, and are inserted into microfluidic channels by tweezer or vacuum pick-and-place motions at fixed positions in which they are efficiently exposed to fluids for conducting assays. Close-field electrostatic attraction is employed to define the position of the elements and enable ready withdrawal of the placing instruments.
    Type: Application
    Filed: December 1, 2015
    Publication date: June 9, 2016
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood