Abstract: Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic silane material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms.

Abstract: A device for limiting sample evaporation. The device has a vessel body having a body exterior surface and at least one body interior surface. The at least one body interior surface has one or more walls defining a first chamber, a second chamber and a conduit means. The first chamber has a first chamber opening for receiving at least one of the groups selected from a sample and a sample dispensing device. The first chamber opening defines the border of the body exterior surface and the body interior surface. The conduit means is in communication with the first chamber and the second chamber for receiving at least one of the group consisting of sample from said first chamber, a sample from a sample dispensing device and a sample dispensing device passing through or into the conduit means for placing the sample in the conduit means or into the second chamber.

Abstract: Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic diene material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms.

Abstract: Methods and systems for analyzing samples using multi-dimensional chromatography are disclosed.

Abstract: A method for extracting at least one target compound from a sample includes injecting an overloaded amount of the sample into a chromatographic conduit (110), and flowing a solvent having a time-varying composition through the conduit (110). An apparatus for extracting at least one target compound from a sample includes a chromatography module, a mass-spectrometry module in fluid communication with the chromatography module to receive a portion of an eluent from the chromatography module, and a control unit in communication with the chromatography module and the mass-spectrometry module.

Abstract: Methods and systems for analyzing samples using multi-dimensional chromatography are disclosed.

Abstract: The invention provides methods of isolating, purifying, analyzing and/or detecting, functionalized macromolecules, e.g., peptides, phosphopeptides, polypeptides, proteins, oligonucleotides, or phospholipids in a sample, e.g., a biological mixture, using solid phase extraction with an alumina sorbent packed in a micro-elution plate.

Abstract: A device for limiting sample evaporation. The device has a vessel body having a body exterior surface and at least one body interior surface. The at least one body interior surface has one or more walls defining a first chamber, a second chamber and a conduit means. The first chamber has a first chamber opening for receiving at least one of the groups selected from a sample and a sample dispensing device. The first chamber opening defines the border of the body exterior surface and the body interior surface. The conduit means is in communication with the first chamber and the second chamber for receiving at least one of the group consisting of sample from said first chamber, a sample from a sample dispensing device and a sample dispensing device passing through or into the conduit means for placing the sample in the conduit means or into the second chamber.

Abstract: Methods and systems for analyzing samples using multi-dimensional chromatography are disclosed.

Abstract: A method for extracting at least one target compound from a sample includes injecting an overloaded amount of the sample into a chromatographic conduit (110), and flowing a solvent having a time-varying composition through the conduit (110). An apparatus for extracting at least one target compound from a sample includes a chromatography module, a mass-spectrometry module in fluid communication with the chromatography module to receive a portion of an eluent from the chromatography module, and a control unit in communication with the chromatography module and the mass-spectrometry module.

Abstract: Embodiments of the present invention are directed to articles of manufacture, devices, methods and apparatus for performing liquid chromatography featuring a chromatographic sorbent having one or more pentafluorophenyl groups, wherein said one or more pentafluorophenyl groups are a bonded phase on a sorbent selected from the group comprising silica, organic polymers or hybrid organic silane material and said pentafluorophenyl groups are in a mono-, bi-, and tridentate forms.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: The present invention provides methods for enhancing chemical reactions of molecules, e.g., biomolecules, with destructible surfactants. The chemical reactions may involve and/or be associate with analysis, e.g., solubilizing, separating, purifying and/or characterizing the molecules. In one aspect, the anionic surfactants of the present invention may be selectively broken up at relatively low pH. The resulting breakdown products of the surfactants may be removed from the molecule/sample with relative ease. The invention has applicability in a variety of analytical techniques.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter; and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the well having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter within the well; a bed of sorbent particles within the well below the first filter, and a second filter having a diameter smaller than the first filter within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.

Abstract: A solid phase extraction (SPE) device having a reservoir with an opening; a well comprising an internally tapered wall, the wall having a wider interior diameter at an end closest to the opening than at an exit spout; a first filter placed within the well; a bed of sorbent particles placed within the well below the first filter; and a second filter having a diameter smaller than the first filter placed within the well below the bed of sorbent particles and above the exit spout is provided. A method of performing SPE using the device is also provided.