Patents by Inventor Martin Schulein
Martin Schulein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20030175940Abstract: The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.Type: ApplicationFiled: March 31, 2003Publication date: September 18, 2003Applicant: Novozymes A/SInventors: Sanne O. Schroder Glad, Carsten Andersen, Martin Schulein, Hanne Dela, Torben Peter
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Patent number: 6613528Abstract: The invention relates to a ellulose film comprising microfibrillated cellulose and to the use of it for screening of a biological compound and nucleic acids encoding a biological compound.Type: GrantFiled: May 20, 2002Date of Patent: September 2, 2003Assignee: Novozymes A/SInventors: William Helbert, Henri Dominique Chanzy, Steffen Ernst, Martin Schulein, Tommy Lykke Husum, Lars Kongsbak
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Patent number: 6607902Abstract: A variant of a cell-wall degrading enzyme having a beta-helix structure, which variant holds at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterising the stack as interior or exterior; characterising the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.Type: GrantFiled: July 19, 2001Date of Patent: August 19, 2003Assignee: Novozymes A/SInventors: Sanne O Schrøder Glad, Carsten Andersen, Martin Schulein, Torben Peter Frandsen
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Publication number: 20030119167Abstract: The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component which is immunoreactive with an antibody raised against a highly purified ˜43 kD endoglucanase derived from Humicola insolens, DSM 1800, or which is homogenous to said ˜43 kD endoglucanase, may be employed in the treatment cellulose-containing fabrics for harshness reduction or color clarification or to provide a localized variation in the color of such fabrics, or it may be employed in the treatment of paper pulp.Type: ApplicationFiled: May 3, 2002Publication date: June 26, 2003Applicant: Novozymes A/SInventors: Grethe Rasmussen, Jan Moller Mikkelsen, Martin Schulein, Shamkant Anant Patkar, Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
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Patent number: 6566114Abstract: Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO:2 or their homologues may be derived from e.g. Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.Type: GrantFiled: June 24, 1999Date of Patent: May 20, 2003Assignee: Novozymes, A/SInventors: Markus Sakari Kauppinen, Martin Schülein, Kirk Schnorr, Lene Nonboe Andersen, Mads Eskelund Bjørnvad
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Publication number: 20030092097Abstract: Described is a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:Type: ApplicationFiled: March 3, 1999Publication date: May 15, 2003Applicant: NOVOZYME A/SInventors: KIM VILBOUR ANDERSEN, MARTIN SCHULEIN, LARS CHRISTIANSEN, BO DAMGAARD, CLAUS VON DER OSTEN
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Publication number: 20030087415Abstract: The present invention relates to transformed bacterial hosts capable of expressing a pectate lyase enzyme endogenous to a strain of Thermotoga maritima, especially a Bacillus or E. coli host cell, is useful in a method for producing the Thermotoga maritima pectate lyase. The Thermotoga maritima pectate lyase is useful for industrial use, e.g. for treatment of textiles.Type: ApplicationFiled: April 12, 2001Publication date: May 8, 2003Applicant: Novozymes A/SInventors: Jens Toenne Andersen, Lars Kongsbak, Martin Schulein, Mads Eskelund Bjornvad
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Publication number: 20030054534Abstract: An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.Type: ApplicationFiled: July 11, 2001Publication date: March 20, 2003Applicant: Novozymes A/SInventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisgard-Frantzen, Martin Schulein, Per Linaa Jorgensen, Mads E. Bjoernvad
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Publication number: 20030054539Abstract: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence 1 (SEQ ID NO:79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa 1 2 3 4 5 6 7 8 9 Xaa Cys Xaa Trp Xaa 10 11 12 13 14Type: ApplicationFiled: December 10, 2001Publication date: March 20, 2003Applicant: Novozymes A/SInventors: Martin Schulein, Hanne Dela, Lene Nonboe Andersen, Soren Flensted Lassen, Markus Sakari Kauppinen, Lene Lange, Ruby Ilum Nielsen, Shinobu Takagi, Michiko Ihara
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Publication number: 20030049707Abstract: The invention relates to a ellulose film comprising microfibrillated cellulose and to the use of it for screening of a biological compound and nucleic acids encoding a biological compound.Type: ApplicationFiled: May 20, 2002Publication date: March 13, 2003Applicant: Novozymes A/SInventors: William Helbert, Martin Schulein, Hanne Dela, Henri Dominique Chanzy, Steffen Ernst, Tommy Lykke Husum, Lars Kongsbak
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Publication number: 20030032162Abstract: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.Type: ApplicationFiled: February 16, 2001Publication date: February 13, 2003Applicant: Novozymes A/SInventors: Kirk Schnorr, Per Lina Jorgensen, Martin Schulein
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Publication number: 20030026810Abstract: Novel isolated enzymes exhibiting rhamnogalacturonan hydrolase activity and belonging to a glycosyl hydrolase family other than family 28 are obtained and cloned from bacterial strains, for example, Bacillus licheniformis, Bacillus subtilis, Bacillus halodurans, Bacillus agaradhaerens, Caldicellulosiruptor, Streptomyces and Sorangium.Type: ApplicationFiled: April 19, 2002Publication date: February 6, 2003Applicant: Novozymes A/SInventors: Per Lina Jorgensen, Kirk Schnorr, Lene Nonboe Andersen, Martin Schulein, Hanne Dela, Helle Outtrup
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Publication number: 20030022807Abstract: The present invention relates to xyloglucanases belonging to family 5 of glycosyl hydrolases are derived from strains of Paenibacillus, especially from strains of Paenibacillus pabuli. The xyloglucanases show high performance in conventional liquid detergent compositions.Type: ApplicationFiled: March 1, 2001Publication date: January 30, 2003Applicant: Novozymes A/SInventors: Reinhard Wilting, Mads Eskelund Bjornvad, Markus Sakari Kauppinen, Martin Schulein, Hanne Dela
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Publication number: 20030017575Abstract: A variant of a cell-wall degrading enzyme having a beta-helix structure, which variant holds at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variant of a wild-type parent pectate lyase (EC 4.2.2.Type: ApplicationFiled: July 19, 2001Publication date: January 23, 2003Applicant: Novozymes A/SInventors: Sanne O. Schroder Glad, Carsten Andersen, Martin Schulein, Hanne Dela, Torben Peter Frandsen
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Patent number: 6500658Abstract: An isolated or purified polypeptide having xyloglucanase activity which is obtained from a strain of the genus Malbranchea and has xyloglucanase activity in the pH range 4-11, measured at 50° C.; and/or a molecular mass of 25±10 kDa, as determined by SDS-PAGE; and/or an isoelectric point (pI) in the range 3-5; and/or an N-terminal sequence Ala-Asp-Phe-Cys-Gly-Gln-Xaa-Asp-Ser-Glu-Gln-Ser-Gly-Pro-Tyr-Ile-Val-Tyr-Asn-Asn-Leu is useful in industrial applications such as in laundry detergent compositions and for treatment of textiles.Type: GrantFiled: September 1, 2000Date of Patent: December 31, 2002Assignee: Novozymes, A/SInventors: Wenping Wu, Martin Schulein, Markus Sakari Kauppinen, Mary Ann Stringer
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Patent number: 6468391Abstract: Sanitary paper with improved softness (lower stiffness) can be obtained without significant loss of paper strength by using a papermaking pulp which is treated with a certain type of cellulase component. The cellulase component in question is characterized by not containing a cellulose-binding domain (CBD), and is more effective for making softer sanitary paper than a conventional cellulase preparation which contains a mixture of various cellulase components with and without a CBD.Type: GrantFiled: June 25, 1998Date of Patent: October 22, 2002Assignee: Novozymes A/SInventors: Masaki Sharyo, Hiromichi Sakaguchi, Masahiro Ohishi, Mamoru Takahashi, Katsunori Kida, Hitoshi Tamagawa, Martin Schulein, Neal Franks
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Publication number: 20020142438Abstract: The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.Type: ApplicationFiled: February 7, 2002Publication date: October 3, 2002Applicant: Novozymes A/SInventors: Lene Nonboe Andersen, Martin Schulein, Hanne Dela, Niels Erik Krebs Lange, Mads Eskelund Bjornvad, Soren Moller, Sanne O. Schroder Glad, Markus Sakari Kauppinen, Kirk Schnorr, Lars Kongsbak
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Patent number: 6429000Abstract: Pectin degrading enzymes derived from or endogeneous to Bacillus licheniformis or other Bacillus species which are at least 99% homologous to Bacillus licheniformis based on aligned 16S rDNA sequences have optimum activity at pH higher than 8. The pectin degrading enzymes belongs to the enzyme classes pectate lyases (EC 4.2.2.2), pectin lyases (EC 4.2.2.10) and polygalacturonases (EC 3.2.1.15) and are useful in industrial processes under alkaline conditions such as in textile processing and as an active ingredient eg in laundry detergents and hard surface cleaning products.Type: GrantFiled: September 26, 2000Date of Patent: August 6, 2002Assignee: Novozymes A/SInventors: Lene Nonboe Andersen, Martin Schülein, Niels Erik Krebs Lange, Mads Eskelund Bjørnvad, Kirk Schnorr
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Patent number: 6426189Abstract: The invention relates to a cellulose film comprising microfibrillated cellulose and to the use of it for screening of a biological compound and nucleic acids encoding a biological compound.Type: GrantFiled: September 29, 2000Date of Patent: July 30, 2002Assignee: Novozymes A/SInventors: William Helbert, Henri Dominique Chanzy, Steffen Ernst, Martin Schulein, Tommy Lykke Husum, Lars Kongsbak
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Patent number: 6399347Abstract: The present invention is directed to isolated enzymes exhibiting rhamnogalacturonan hydrolase activity, to nucleic acids encoding such enzymes, and to methods of producing such enzymes. The present invention is also related to detergent and animal feed compositions, compositions and methods for treating cellulosic materials, such as fibers, compositions and methods for preparing fruit or vegetable products, and compositions and methods for preparing wine and juice.Type: GrantFiled: May 13, 1999Date of Patent: June 4, 2002Assignee: Novozymes A/SInventors: Per Linå Jørgensen, Kirk Schnorr, Lene Nonboe Andersen, Martin Schülein, Helle Outtrup