Patents by Inventor Martin Schulein

Martin Schulein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6001639
    Abstract: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in posit
    Type: Grant
    Filed: May 21, 1996
    Date of Patent: December 14, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Lene Nonboe Andersen, S.o slashed.ren Flensted Lassen, Markus Sakari Kauppinen, Lene Lange, Ruby Iium Nielsen, Michiko Ihara, Shinobu Takagi
  • Patent number: 5981253
    Abstract: The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: November 9, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein
  • Patent number: 5972872
    Abstract: The present invention relates to detergent compositions comprising an enzyme preparation with substantial cellulytic activity, especially endoglucanase activity, at alkaline conditions which is enzyme composition is derived from or producible by a fungus selected from the Basidiomycetous families Coprinaceae and Bolbitiaceae, preferably from the group of strains belonging to the genera Psathyrella, Podaxis, Panaeolus, Coprinus and Bolbitius, more preferably from the group of strains belonging to the species Coprinus micaceus, Coprinus domesticus, Coprinus ephemerus, Coprinus disseminatus, Coprinus radians, Coprinus picaceus, Coprinus frisei, Coprinus subimpatiens, Psathyrella candolleana, Psathyrella prona, Panaeolus semiovatus, Podaxis pistillaris and Bolbitius aleuriatus; and a method for providing a stone-washed look to textiles, especially to cellulosic fabric such as denim, using the mentioned enzyme preparation; and a method for improving the drainage of paper pulp or the de-inking of recycled paper usi
    Type: Grant
    Filed: June 6, 1997
    Date of Patent: October 26, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Lene Lange, Jack Bech Nielsen, Martin Schulein
  • Patent number: 5958083
    Abstract: This invention relates to a method of forming localized variation of color density in the surface of a dyed cellulosic fabric, and to a composition for use in the method.
    Type: Grant
    Filed: March 2, 1998
    Date of Patent: September 28, 1999
    Assignee: Novo Nordisk A/A
    Inventors: Masahiro Onishi, Merete Fich, Annette Hanne Toft, Martin Schulein
  • Patent number: 5948672
    Abstract: A cellulase preparation consisting essentially of a homogeneous endoglucanase component which is immunoreactive with an antibody raised against a highly purified .about.43 kD endoglucanase derived from Humicola insolens, DSM 1800, or which is homogeneous to said .about.43 kD endoglucanase, may be employed in the treatment cellulose-containing fabrics for harshness reduction or color clarification or to provide a localized variation in the color of such fabrics, or it may be employed in the treatment of paper pulp.
    Type: Grant
    Filed: February 14, 1995
    Date of Patent: September 7, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Grethe Rasmussen, Jan M.o slashed.ller Mikkelsen, Martin Schulein, Shamkant Anant Patkar, Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
  • Patent number: 5945327
    Abstract: An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.
    Type: Grant
    Filed: June 6, 1997
    Date of Patent: August 31, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein, Per Linaa Jorgensen, Mads E. Bjoernvad
  • Patent number: 5922586
    Abstract: An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.
    Type: Grant
    Filed: September 13, 1996
    Date of Patent: July 13, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein, Per Linaa Jorgensen, Mads E. Bjoernvad
  • Patent number: 5919691
    Abstract: DNA sequences are disclosed encoding an enzyme exhibiting endoglucanase activity. The endoglucanase is useful in a variety of industrial processes requiring an alkaline cellulase.
    Type: Grant
    Filed: March 20, 1997
    Date of Patent: July 6, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Karen Margrethe Oxenb.o slashed.ll, Lene Nonboe Andersen, S.o slashed.ren Flensted Lassen, Markus Sakari Kauppinen, Jack Bech Nielsen
  • Patent number: 5916796
    Abstract: This invention relates to an enzyme which exhibits cellulase activity and is producible by a strain of Bacillus sp., NCIMB 40250, or a related Bacillus sp. strain, or a derivative of said cellulase. This invention also relates to an enzyme which comprises a core region derived from an endoglucanase combined with a cellulose-binding domain derived from another cellulase, or which comprises a core region derived from another cellulase combined with a cellulose-binding domain derived from an endoglucanase.
    Type: Grant
    Filed: July 27, 1992
    Date of Patent: June 29, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Per Lin.ang. J.o slashed.rgensen, Martin Schulein, Christian Hansen
  • Patent number: 5912157
    Abstract: The present invention relates to cellulases derived from the strain Myceliophthora thermophila and variants thereof comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having a substrate binding cleft of a depth of at least 12 .ANG., exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.
    Type: Grant
    Filed: September 9, 1996
    Date of Patent: June 15, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Claus von der Osten, Martin Schulein
  • Patent number: 5866392
    Abstract: Enzyme exhibiting cellobiose oxidase activity, which enzyme has a relative activity of at least 70% at a pH of about 9 and a temperature of about 50.degree. C. An enzymatic agent comprising cellobiose oxidase and an endoglycanase and/or oxidoreductase. A process of bleaching paper pulp using cellobiose oxidase.
    Type: Grant
    Filed: June 24, 1996
    Date of Patent: February 2, 1999
    Assignee: Novo Nordisk A/S
    Inventors: Charlotte Schou, Martin Schulein, Thomas Vollmond
  • Patent number: 5792641
    Abstract: A cellulase variant of a parent cellulase, e.g. a cellulase classified in family 45 such as a Humicola insolens 43 kD endoglucanase, comprising a cellulose binding domain (CBD), a catalytically active domain (CAD) and a region linking the cellulose binding domain and catalytically active domain (the linking region), wherein one more amino acid residues of the CBD, CAD or linking region is deleted or substituted by one or more amino acid residues and/or one or more amino acids are added to the linking region and/or another CBD is added at the opposite end of the catalytically active domain, has improved properties as regards e.g. alkaline activity, compatibility with detergent composition ingredients, particulate soil removal, color clarification, defuzzing, depilling, harshness reduction, and sensitivity to anionic surfactants and peroxidase bleaching systems and is useful e.g. in detergent compositions, for textile treatment, in paper pulp processing, for animal feed and for stone washing of jeans.
    Type: Grant
    Filed: May 5, 1995
    Date of Patent: August 11, 1998
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Henrik Fredholm, Carsten Mailand Hjorth, Grethe Rasmussen, Egon Nielsen, Peter Rosholm
  • Patent number: 5776757
    Abstract: A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and for reducing the rate at which such fabrics become harsh comprises about 40% or more (based on the total protein content) of an endoglucanase component with a pH optimum of about 7.5-10.0 with a high CMC-endoase activity and affinity towards cellulose and essentially no cellobiohydrolase activity. The cellulase composition is endogenous to a strain of Humicola, Myceliophthora, or Fusarium. A method for the recombinant production of said cellulase preparation is provided.
    Type: Grant
    Filed: May 30, 1995
    Date of Patent: July 7, 1998
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Kirsten B.o slashed.egh Levring
  • Patent number: 5770424
    Abstract: The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.
    Type: Grant
    Filed: August 16, 1996
    Date of Patent: June 23, 1998
    Assignee: NovoNordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein, Per Linaa Jorgensen
  • Patent number: 5741693
    Abstract: The present invention relates to proteases obtained from a strain of Bacillus sp. AC13, and having (a) an apparent molecular weight of approximately 30 kD as determined by SDS-PAGE; (b) an isoelectric point of approximately 9.3 as determined by isoelectric focusing on LKB Ampholine PAG plates; (c) a pH optimum above 10 determined at 25.degree. C. with casein as substrate; (d) a temperature optimum in the range of 45.degree.-55.degree. C. determined at pH 9.5 with casein as substrate; and (e) immunochemical properties identical or partially identical to those of a protease derived from Bacillus sp. AC13, NCIMB No. 40482.
    Type: Grant
    Filed: November 30, 1994
    Date of Patent: April 21, 1998
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein
  • Patent number: 5696068
    Abstract: The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: December 9, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Helle Outtrup, Claus Dambmann, Arne Agerlin Olsen, Henrik Bisg.ang.rd-Frantzen, Martin Schulein
  • Patent number: 5691178
    Abstract: A cellulase preparation useful for reducing the harshness of cotton-containing fabrics and for reducing the rate at which such fabrics become harsh comprises about 40% or more (based on the total protein content) of an endoglucanase component with a pH optimum of about 7.5-10.0 with a high CMC-endoase activity and affinity towards cellulose and essentially no cellobiohydrolase activity. The cellulase composition is endogenous to a strain of Humicola, Myceliophthora, or Fusarium.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: November 25, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Kirsten B.o slashed.egh Levring
  • Patent number: 5648263
    Abstract: The present invention relates to methods of reducing the rate at which a cotton-containing fabric becomes harsh or of reducing the harshness of a cotton-containing fabric, comprising treating the cotton-containing fabric with a cellulase preparation obtained from a fungus, wherein the cellulase preparation comprises a substantially homogeneous endoglucanase component which: (a) has a pH optimum of about 7.5-10.0; (b) exhibits a CMC endoase activity of at least about 10 CMC-endoase units per mg of total protein; (c) exhibits a CAVU activity of at least about 50% at a pH of 7.0-12.0; and (d) has essentially no cellobiohydrolase activity; wherein at least about 50% by weight of the total cellulase content of the cellulase preparation is the endoglucanase component.
    Type: Grant
    Filed: May 30, 1995
    Date of Patent: July 15, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Kirsten B.o slashed.egh Levring
  • Patent number: 5610048
    Abstract: The xylanase is characterized by several partial amino acid sequences and is immunoreactive with an antibody raised against a purified xylanase derived from Humicola insolens, DSM 1800. This xylanase preparation is practically free of cellulase xylanase and is well suited for treatment of paper pulp, as a baking agent and as an additive to fodder.
    Type: Grant
    Filed: September 21, 1993
    Date of Patent: March 11, 1997
    Assignee: Novo Nordisk A/S
    Inventors: Martin Schulein, Torben Halkier, Hans P. Heldt-Hansen, Henrik Dalb.o slashed.ge, Lars S. Pedersen
  • Patent number: 4478939
    Abstract: SPS--A novel pectic-like polysaccharide derived from soy plant cell walls characterized by capability to bind to proteins.SPS-ase--The carbohydrase complex capable of decomposing SPS into decomposition products incapable of attaching to protein, and method for producing SPS-ase by cultivation of an SPS-ase producing microorganism for which preferred microorganism strains are Aspergillus aculeatus CBS 101.43 and Aspergillus japonicus IFO 4408.
    Type: Grant
    Filed: December 24, 1981
    Date of Patent: October 23, 1984
    Assignee: Novo Industri A/S
    Inventors: Jens L. Adler-Nissen, Henrik Gurtler, Georg W. Jensen, Hans A. S. Olsen, Steen Riisgaard, Martin Schulein