Abstract: A cargo cover system for use with and connectable to a cargo carrier having at least a first mounting member and a second mounting member, comprising a cargo cover frame and a cargo carrier cover, the cargo cover frame having elongated first and second side support members, each side support member having first and second ends, a first and second angles forming a middle portion between the two ends, a receiver hole located between the two angles in the middle portion, a side mounting member connecting apparatus and a side locator insertable into the corresponding cargo carrier mounting member, and a fastener insertable through the receiver hole and the side locator and is releasably affixable to the side mounting member connecting apparatus, a first side upper support member, a first side lower support member, a second side upper support member, and a second side lower support member.

Abstract: This invention relates to a heavy-duty cargo carrier, specifically designed for electric bicycles and other heavy bicycles that includes a ramp to help facilitate loading the bicycles and a tilting twin tube where the tilting twin tube allows for the twin tubes to be either locked horizontally or to pivot into an upright, vertical position where they can be locked in place vertically.

Abstract: A trailer accessory for a jack tower where the accessory is a table, the table rests on three bases, the first base is a top plate across the top of the jack tower and the second and third bases are first and second arms that extend outwardly from the top of the jack tower, these first and second arms end at or past the end of the table and are connected to uprights where the first upright connects to the first arm at one end and to a first leg of an A-frame trailer at a second end, and the second upright connects to the second arm at one end and to a second leg of the A-frame trailer at the other end, thus creating a solid base for the table.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The invention relates to clinical applications of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage (ChIC) and cleavage under targets & release using nuclease (CUT&RUN®)). The methods may be used to detect and quantitate the presence of epigenetic modifications and mutations on nucleosomes (histones and/or DNA) from biological samples, monitor changes in the status of modifications and mutations, monitor the effectiveness of epigenetic and mutation therapies, select suitable treatments for a disease, determine the prognosis of a subject, identify biomarkers of a disease, and screen for agents that modify epigenetic or mutation status. The invention further relates to kits for use in the methods of the invention.

Abstract: The invention relates generally to methods for preparing recombinant nucleosomes. In particular, the invention relates to methods for ligating a histone peptide onto a fully assembled recombinant nucleosome. The invention further relates to modified core histone proteins, histone peptides to be ligated to the modified core histone proteins, and fully assembled recombinant nucleosomes and libraries of recombinant nucleosomes prepared by the methods of the invention.

Abstract: An exercise apparatus having a stationary frame, a handlebar assembly, a frontal-torso assembly, a resistance assembly, a rotatable crankset having a pair of crankarms with pedals mounted thereon where the crankset is positioned rearward of the handlebar assembly and the frontal-torso assembly.

Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.

Abstract: A storage stand having a base with a main tube having a first end, a middle portion and a second end with at least one wing receiver affixable to the main tube, a pair of outer stand tubes each having a first end, a middle portion and a second end; a pair of leg connectors to connect the main tube first and second ends to the middle portions of the pair of outer stand tubes; feet located at each of the outer stand tubes first and second ends; at least one wing secureable to the at least one wing receiver; and at least one payload carrying member affixable to the at least one wing.

Abstract: An exercise apparatus having a stationary frame, a handlebar assembly, a frontal-torso assembly, a resistance assembly, a rotatable crankset having a pair of crankarms with pedals mounted thereon where the crankset is positioned rearward of the handlebar assembly and the frontal-torso assembly.

Abstract: The invention relates generally to methods for preparing recombinant nucleosomes. In particular, the invention relates to methods for ligating a histone peptide onto a fully assembled recombinant nucleosome.

Abstract: The present invention is a cargo carrier 100 for attachment to a mounted tire having a continuous curved bridge where the bridge has a first end 110 followed by a first concave section 182, a convex middle section 180 and a second concave section 185 followed by a second end 130; there is an arm member 140 extending upwardly from each of the first 110 and second ends 130 with at least one payload carrying member 200 connectable to the arm members 140 to secure a payload to the cargo carrier 100; there is at least one tire mount 300 at a curved bridge bottom 188 that rests on an upper surface of the tire and at least one securement apparatus 310 to secure the cargo carrier 100 to the tire.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The invention relates to clinical applications of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage (ChIC) and cleavage under targets & release using nuclease (CUT&RUN®)). The methods may be used to detect and quantitate the presence of epigenetic modifications and mutations on nucleosomes (histones and/or DNA) from biological samples, monitor changes in the status of modifications and mutations, monitor the effectiveness of epigenetic and mutation therapies, select suitable treatments for a disease, determine the prognosis of a subject, identify biomarkers of a disease, and screen for agents that modify epigenetic or mutation status. The invention further relates to kits for use in the methods of the invention.

Abstract: A storage stand having a base with a main tube having a first end, a middle portion and a second end with at least one wing receiver affixable to the main tube, a pair of outer stand tubes each having a first end, a middle portion and a second end; a pair of leg connectors to connect the main tube first and second ends to the middle portions of the pair of outer stand tubes; feet located at each of the outer stand tubes first and second ends; at least one wing secureable to the at least one wing receiver; and at least one payload carrying member affixable to the at least one wing.

Abstract: The present invention is a cargo carrier 100 for attachment to a mounted tire having a continuous curved bridge where the bridge has a first end 110 followed by a first concave section 182, a convex middle section 180 and a second concave section 185 followed by a second end 130; there is an arm member 140 extending upwardly from each of the first 110 and second ends 130 with at least one payload carrying member 200 connectable to the arm members 140 to secure a payload to the cargo carrier 100; there is at least one tire mount 300 at a curved bridge bottom 188 that rests on an upper surface of the tire and at least one securement apparatus 310 to secure the cargo carrier 100 to the tire.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The current invention teaches a cargo management apparatus for use on a vehicle tongue where the apparatus has a bottom plate, a first riser member with a bottom end, a middle portion and a top end, and a second riser member with a bottom end, a middle portion and a top end, where the bottom end of the first riser member and the bottom end of the second riser member are secured to a top side of the bottom plate; a top member, where the top end of the first riser member and the top end of the second riser member are secured to a bottom side of the top plate, this base then further having an accessory mount securedly affixed on top of the base with the ability to secure a variety of cargo carrying accessories.