Patents by Inventor Marvin H. Caruthers

Marvin H. Caruthers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20150225442
    Abstract: The present invention provides nucleosides comprising a sterically hindered silyl protecting group on the exocyclic nitrogen atom of the nucleobase, oligonucleotides comprising the same, and methods for producing the same. In particular, the silyl protecting group is a sufficiently sterically hindered such that it is stable to a various oligonucleotide synthesis procedures.
    Type: Application
    Filed: August 3, 2013
    Publication date: August 13, 2015
    Applicant: The Regents of the University of Colorado, a Body Corporate
    Inventors: Marvin H. Caruthers, Subhadeep Roy
  • Patent number: 9067961
    Abstract: Aspects of the invention include 2? protected nucleoside monomers that are protected at the 2? site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: June 30, 2015
    Assignees: Agilent Technologies, Inc., The Regents of the University of Colorado Denver
    Inventors: Douglas J. Dellinger, Agnieszka B. Sierzchala, John Turner, Joel Myerson, Zoltan Kupihar, Fernando Ferreira, Marvin H. Caruthers, Geraldine F. Dellinger
  • Patent number: 8552174
    Abstract: Methods of deprotecting polynucleotides are disclosed. One aspect of the method of deprotecting polynucleotides, among others, includes: providing a polynucleotide, wherein the polynucleotide includes at least one nucleotide monomer that has at least one protecting group selected from the following: a base having a protecting group, a 2?-hydroxyl protecting group, and a combination thereof, and deprotecting at least one of the protecting groups of the polynucleotide by introducing the polynucleotide to a solution including an ?-effect nucleophile.
    Type: Grant
    Filed: March 23, 2006
    Date of Patent: October 8, 2013
    Assignees: Agilent Technologies, Inc., The Regents of the University of Colorado
    Inventors: Douglas J. Dellinger, Zoltan Timar, Agnieszka Sierzchala, Geraldine Dellinger, Marvin H. Caruthers, Joel Myerson
  • Patent number: 8309706
    Abstract: The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with ?-effect nucleophiles. The ?-effect nucleophile irreversibly cleave the carbonate protecting groups while simultaneously oxidizing the internucleotide phosphite triester linkage to a phosphodiester linkage. The procedure may be carried out in aqueous solution at neutral to mildly basic pH. The method eliminates the need for separate deprotection and oxidation steps, and, since the use of acid to remove protecting groups is unnecessary, acid-induced depurination is avoided. Fluorescent or other readily detectable carbonate protecting groups can be used, enabling monitoring of individual reaction steps during oligonucleotide synthesis. The invention is particularly useful in the highly parallel, microscale synthesis of oligonucleotides.
    Type: Grant
    Filed: September 6, 2007
    Date of Patent: November 13, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Marvin H. Caruthers, Jason R. Betley
  • Publication number: 20120238737
    Abstract: Aspects of the invention include 2? protected nucleoside monomers that are protected at the 2? site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
    Type: Application
    Filed: May 31, 2012
    Publication date: September 20, 2012
    Applicant: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Agnieszka Sierzchala, John Turner, Joel Myerson, Zoltan Kupihar, Fernando Ferreira, Marvin H. Caruthers, Geraldine F. Dellinger
  • Patent number: 8242258
    Abstract: Aspects of the invention include 2? protected nucleoside monomers that are protected at the 2? site with orthoester-type protecting groups. The 2? protected monomers also include a second, aryl carbonate-type, protecting group. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
    Type: Grant
    Filed: December 3, 2007
    Date of Patent: August 14, 2012
    Assignees: Agilent Technologies, Inc., The Regents of the University of Colorado
    Inventors: Douglas J. Dellinger, Brian Stell, Marvin H. Caruthers
  • Patent number: 8202983
    Abstract: Aspects of the invention include 2? protected nucleoside monomers that are protected at the 2? site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: June 19, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Agnieszka Sierzchala, John Turner, Joel Myerson, Zoltan Kupihar, Fernando Ferreira, Marvin H. Caruthers, Geraldine F. Dellinger
  • Patent number: 8202985
    Abstract: Nucleotide monomers, polynucleotides, methods of making each, and methods of deprotecting each, are disclosed. An embodiment of the nucleotide monomer, among others, includes a nucleotide monomer having a heterobase protecting group selected from structures I through III as described herein. An embodiment of the polynucleotide, among others, includes a plurality of nucleotide moieties having a heterobase protecting group selected from one of structures I through III as described herein.
    Type: Grant
    Filed: March 23, 2006
    Date of Patent: June 19, 2012
    Assignees: Agilent Technologies, Inc., The Regents of the University of Colorado
    Inventors: Douglas J. Dellinger, Zoltan Timar, Agnieszka Sierzchala, Geraldino Dellinger, Marvin H. Caruthers
  • Patent number: 8097711
    Abstract: Embodiments of the invention include nucleotide and nucleoside monomers protected at the 5?- or 3?-hydroxyls with thioether substituted aryl carbonate protecting groups. In certain cases, the carbonate protecting groups include an aryl moiety, e.g., a phenyl group, attached to the carbonate, where the aryl moiety further includes a thioether group, e.g., an alkyl or aryl thioether group, bound directly to the aryl ring. Aspects of the invention further include methods of synthesizing nucleic acids, e.g., oligonucleotides, using such protected nucleoside monomer monomers, as well as nucleic acids produced using methods of the invention and compositions thereof.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: January 17, 2012
    Assignee: Agilent Technologies, Inc.
    Inventors: Zoltan Timar, Zoltan Kupihar, Douglas J. Dellinger, Marvin H. Caruthers
  • Patent number: 7999087
    Abstract: Nucleoside monomers, nucleic acids, e.g., oligonucleotides and polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein. Aspects of the invention include 2? silyl containing thiocarbonate protecting groups. Corresponding compositions and methods are provided.
    Type: Grant
    Filed: November 15, 2007
    Date of Patent: August 16, 2011
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Agnieska Sierzchala, Marvin H. Caruthers, Geraldine F. Dellinger
  • Patent number: 7790387
    Abstract: In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
    Type: Grant
    Filed: September 24, 2007
    Date of Patent: September 7, 2010
    Assignees: Agilent Technologies, Inc., Regents of the University of Colorado
    Inventors: Douglas J. Dellinger, Zoltan Timar, Joel Myerson, Geraldine Dellinger, Marvin H. Caruthers
  • Patent number: 7759471
    Abstract: Nucleotide monomers, polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein.
    Type: Grant
    Filed: March 23, 2006
    Date of Patent: July 20, 2010
    Assignees: Agilent Technologies, Inc., Regents of the University Of Colorado
    Inventors: Douglas J. Dellinger, Zoltan Timar, Agnieszka Sierzchala, Geraldine Dellinger, Marvin H. Caruthers
  • Publication number: 20100010069
    Abstract: The present invention provides a novel method for solid-phase phosphoramidite based synthesis of borane phosphonate DNA. Also provided are novel phosphoramidite molecules, novel extended length borane phosphonate nucleic acid compounds, and methods of use thereof.
    Type: Application
    Filed: March 9, 2007
    Publication date: January 14, 2010
    Applicant: The Regents of the University of Colorodo
    Inventors: Heather Brummel McCuen, Agnieszka B. Sierzchala, Marvin H. Caruthers
  • Publication number: 20090209479
    Abstract: Aspects of the invention include 2? protected nucleoside monomers that are protected at the 2? site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
    Type: Application
    Filed: May 9, 2008
    Publication date: August 20, 2009
    Inventors: Douglas J. Dellinger, Agnieszka Sierzchala, John Turner, Joel Myerson, Zoltan Kupihar, Fernando Ferreira, Marvin H. Caruthers
  • Patent number: 7572907
    Abstract: Methods of forming polynucleotides are disclosed.
    Type: Grant
    Filed: April 29, 2005
    Date of Patent: August 11, 2009
    Assignees: Agilent Technologies, Inc., The Regents of the University of Colorado
    Inventors: Douglas J. Dellinger, Geraldine Dellinger, Marvin H. Caruthers, Zoltan Kupihar
  • Patent number: 7524950
    Abstract: Inkjet printhead solvents and methods of forming an addressable nucleotide array are disclosed.
    Type: Grant
    Filed: April 29, 2005
    Date of Patent: April 28, 2009
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Eric LeProust, Bill Peck, Marvin H. Caruthers, Peter Cogan
  • Publication number: 20090082554
    Abstract: In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
    Type: Application
    Filed: September 24, 2007
    Publication date: March 26, 2009
    Inventors: Douglas J. Dellinger, Zoltan Timar, Joel Myerson, Geraldine Dellinger, Marvin H. Caruthers
  • Patent number: 7427679
    Abstract: Precursors for use in the synthesis of polynucleotides are disclosed. The precursors include a heterocyclic base having an exocyclic amine group and a substituted or unsubstituted triaryl methyl protecting group bound to the exocyclic amine group.
    Type: Grant
    Filed: August 30, 2003
    Date of Patent: September 23, 2008
    Assignee: Agilent Technologies, Inc.
    Inventors: Douglas J. Dellinger, Agnieszka B. Sierzchala, Marvin H. Caruthers
  • Publication number: 20080227964
    Abstract: Nucleoside monomers, nucleic acids, e.g., oligonucleotides and polynucleotides, methods of making each, methods of deprotecting each, and the like are disclosed herein. Aspects of the invention include 2? silyl containing thiocarbonate protecting groups. Corresponding compositions and methods are provided.
    Type: Application
    Filed: November 15, 2007
    Publication date: September 18, 2008
    Inventors: Douglas J. Dellinger, Agnieszka Sierzchala, Marvin H. Caruthers, Geraldine F. Dellinger
  • Publication number: 20080206850
    Abstract: In some embodiments, the present disclosure relates to new phosphoramidite compositions, that have Silyl-containing carbonate or thiocarbonate or ether as 5?-hydroxyl protecting groups useful of the synthesis of DNA, and in particular for the synthesis of long sequences of DNA (e.g., >50 mer). In some embodiments, there are provided methods for simultaneous oxidation of the internucleoside phosphate triester linkages and removal of the 5?-hydroxyl-protecting group, making this process a new 2-step DNA synthesis, that involves the use of peroxyanions in combination with fluoride anions.
    Type: Application
    Filed: February 28, 2007
    Publication date: August 28, 2008
    Inventors: Douglas J. Dellinger, Geraldine F. Dellinger, Marvin H. Caruthers