Abstract: A method for the determination of an analyte which is a) present in a liquid sample 1 suspected of containing the analyte, and b) at least bivalent with respect to simultaneous affinity binding of at least two binding structures BSs. The method comprises formation of an affinity complex that comprises the analyte and an affinity reactant 1 that is immobilized to a solid phase. The method comprises the steps of: (i) providing a microfluidic flow path that comprises a reaction cavity containing a solid phase to which affinity reactant 1 is immobilized, (ii) providing sample 1 upstream of the cavity and flowing it through the cavity for the formation of the affinity complex under flow conditions, (iii) measuring the amount of complex formed in the solid phase by a) incorporating an analytically detectable and soluble affinity reactant 2 that comprises a binding structure BS into the complex subsequent to step (ii), and b) measuring the amount of affinity reactant 2 incorporated.

Abstract: A method and a system for grading the confidence of a result of an experiment that comprises one or more biological and/or chemical reactions and is carried out in a microchannel structure (110a) of a microfluidic device, said confidence determination comprises the steps of: i) detecting within each of at least one liquid detector segment of said microchannel structure (110a) the presence or absence of liquid and/or gas during a period of time for which it is known if liquid and/or gas shall be present and/or absent in the segment, and ii) assigning a lowered confidence to said result if the presence and/or absence of liquid and/or gas found in step (i) is deviating from what it shall be.

Abstract: A microfluidic device that comprises a microchannel structure in which there are one, two or more flow paths (101;201a,b;301a,a?,b) all of which comprises a porous bed I (104,204,304) that is common for all of the flow paths and exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed. The characteristics are that at least one of the flow paths comprises/comprise a second porous bed II (105,205,305) that is placed upstream of porous bed I (104,204,304) and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R. There is also disclosed a method utilizing the device and variant of the device in which the immobilized R is replaced with a generic affinity ligand LI and/or porous bed II exposes a generic ligand LII that may be different from LI.

Abstract: A method of the quantitative determination of an immunoglobulin (Ig) of a certain class (IgX) in a liquid sample comprising the step of forming a complex that comprises a ternary affinity complex in which IgX is sandwiched between two affinity reactants 1 and 2 (R1 and R2, respectively).

Abstract: A method for quantifying n different analytes that are present in one or more liquid samples by performing n different affinity assay formats, each of which is dedicated for a particular one of the analytes. The characteristic feature is that each format is performed in a separate microchannel structures of a microfluidic device that contains at least n microchannel structures, and comprises formation and measurement of an immobilized product (affinity complex) that is formed on a solid phase that is placed in a microcavity of the microchannel structure used for the format in order to quantify the analyte to which the format is dedicated.

Abstract: The present invention relates to a method, a system, a neural network and a computer program product for determining the quality of an analytical process, preferably the confidence value C. The analytical process is performed in a microchannel structure of a microfluidic device, from which data information of the analytic process is acquired by scanning at least one search area of the microchannel structure for signal data. Said search area comprises the result of the analytical process and said acquired data information is stored as an image, one image for each scanned search area.

Abstract: A microfluidic device comprising one, two or more microchannel structures (101a-h), each of which comprises a reaction microcavity (104a-h) intended for retaining a solid phase material in the form of a wet porous bed. Each of said one, two or more microchannel structures comprises the solid phase material in a dry state together with a bed-preserving agent comprising one or more compounds having bed-preserving activity.

Abstract: Method for measuring the signal from a label in a labeled measuring reagent that has been specifically adsorbed to its affinity counterpart in a zone of a porous nanolitre (nl) bed that is present in a microchannel structure of a microfluidic device. The measuring is part of an assay for determining an analyte of a sample by performing one, two or more heterogeneous specific affinity reactions which comprises that said labeled measuring reagent becomes affinity bound to said zone.

Abstract: A collection of one or more microfluidic devices which together carry a plurality of microchannel structures each of which comprises a reaction microcavity (104a-h) in which there is a solid phase with an immobilized affinity ligand L, characterized in that (i) the plurality is divided into sets of microchannel structures, and (ii) the affinity ligand L is directed to the same counterpart (binder, B) independent of set, and (iii) the sets differ in a) the capacity per reaction microcavity and/or the capacity/unit volume of the solid phase in a reaction microcavity for binder B, and/or b) the base matrix of the solid phase between the sets but are equal within each set.

Abstract: A method for transporting in a microfluidic conduit an aliquot of a liquid, possibly containing a substance I, which is dissolved in the liquid and typically exhibits charged groups and/or hydrophobic groups. The method is characterized in that the liquid contains an amphiphilic macromolecular substance.

Abstract: A method for sequence-based diagnosis of a human neoplastic tissue, blood or other body fluid sample, comprises analysing from genomic DNA or cDNA derived from said neoplasia the DNA sequence of a gene encoding a cancer-related protein for the presence of mutations therein, determining from the presence, nature and location of any such mutation or mutations the influence thereof on the biological function of the corresponding protein and thereby on the properties of the neoplasia, and on the basis thereof prognosticating the development of the neoplasia.