Abstract: A power tool chuck includes a chuck body, a longitudinal bore extending along an axis for receiving a tool bit, a plurality of radial slots in communication with the longitudinal bore, a plurality of jaw assemblies, each at least partially received in one of the radial slots and moveable to removably retain the tool bit in the chuck body, and a clamping ring rotatably received over the chuck body and the jaw assemblies. The clamping ring and each jaw assembly together define a first clamping interface configured to cause the jaw assembly to clamp the tool bit a first clamping force up to a first maximum clamping force during a first phase of clamping, and a second clamping interface configured to cause the jaw assembly to clamp the tool bit at a second clamping force that is greater than the first maximum clamping force during a second phase of clamping.

Abstract: The present application discloses methods for synthetic production and cell-free synthesis of single stranded adeno-associated virus (AAV) vectors, for delivery and expression of a transgene in host cells. The present invention also relates to an in vitro process for production of closed-ended DNA vectors and corresponding single stranded AAV DNA vector products synthesized from the closed-ended DNA vectors having nicks.

Abstract: A vacuum tube assembly for removing material is disclosed, including: a vacuum generator configured to generate a vacuum airflow; one or more tubes coupled to the vacuum generator and configured to channel the vacuum airflow; and an actuation mechanism coupled to the one or more tubes, wherein the actuation mechanism is configured to actuate at least one tube from a first position relative to a material stream to a second position relative to the material stream, wherein the first position is farther from the material stream than the second position.

Abstract: The application describes methods for synthetic synthesis and cell-free synthesis of DNA vectors, particularly closed-ended DNA vectors (e.g., ceDNA vectors) having linear and continuous structure for delivery and expression of a transgene. The present invention relates to an in vitro process for production of closed-ended DNA vectors, corresponding DNA vector products produced by the methods and uses thereof, and oligonucleotides and kits useful in the process of the invention. DNA vectors produced using the methods described herein are free from unwanted side effects due to contaminants introduced during production in cell lines, for example, bacterial or insect cell lines. Further provided herein are methods and cell lines for reliable gene expression in vitro, ex vivo and in vivo using the ceDNA vectors synthesized using the methods herein.

Abstract: The invention features polynucleotides encoding a polypeptide including a 3?-stabilizing region and having increased stability compared to wild-type polynucleotides.

Abstract: A lawn mower includes a frame, an operator support, a blade assembly, a motor assembly, a steering assembly, one or more sensors and one or more processors. The frame is supported on rotatable wheels for movement over a ground surface. The operator support is configured to support the entire weight of an operator of the lawn mower during use thereof. The blade assembly comprises one or more blades. The motor assembly is configured to: drive the wheels so as to move the frame along the ground surface; and drive the one or more blades relative to the ground surface to cut grass. The steering assembly is configured to manipulate the steering direction of the wheels. The one or more processors are configured to control one or more components of the lawn mower based on input received from the one or more sensors.

Abstract: A chuck including a chuck body that supports a plurality of jaws. An outer sleeve is axially fixed with respect to the chuck body. A nut is coupled to the outer sleeve, and the nut is movable axially and radially relative to the chuck body. The nut interacts with the jaws such that when the outer sleeve rotates, the nut moves axially and radially relative to the body.

Abstract: Pick quality determination is disclosed, including: using a pressure meter to sense a pressure associated with an airflow through a gripper mechanism of a diverting mechanism over time during a pick operation on a target object; storing the sensed pressure associated with the airflow through the gripper mechanism over time as a pressure sequence associated with the pick operation on the target object; and correlating the pressure sequence with representative pressure sequences associated with corresponding pick quality types to determine whether the pick operation on the target object was successful or not.

Abstract: Pick quality determination is disclosed, including: using a pressure meter to sense a pressure associated with an airflow through a gripper mechanism of a diverting mechanism over time during a pick operation on a target object; storing the sensed pressure associated with the airflow through the gripper mechanism over time as a pressure sequence associated with the pick operation on the target object; and correlating the pressure sequence with representative pressure sequences associated with corresponding pick quality types to determine whether the pick operation on the target object was successful or not.

Abstract: A lawn mower includes a frame, an operator support, a blade assembly, a steering system, a motor assembly, a sensor and one or more processors. The operator support is coupled to the frame and is configured to support the entire weight of an operator of the lawn mower during use thereof. The motor assembly is configured to drive rotatable wheels so as to move the frame along the ground surface and drive at least one blade relative to the ground surface to cut grass. Sensor and processor to detect the an edge between an unmowed area of grass and a mowed area of grass and control the motor assembly stop driving the at least one blade, the wheels, or both based on the input from the sensor.

Abstract: A material picker assembly is disclosed, including: a connector configured to be attached to an actuator device; and a plurality of picker mechanisms, wherein each picker mechanism includes a suspension element that is configured to absorb impact on the picker mechanism by one or more objects.

Abstract: The invention features polynucleotides encoding a polypeptide including a 3?-stabilizing region and having increased stability compared to wild-type polynucleotides.

Abstract: The disclosure provides polynucleotides encoding a polypeptide including a morpholino linker. In some embodiments, the polynucleotides of the invention have increased stability compared to wild-type polynucleotides.

Abstract: An interchangeable electro-mechanical lock core for use with a lock device having a locked state and an unlocked state is disclosed. The interchangeable electromechanical lock core may include a moveable plug having a first position relative to a lock core body which corresponds to the lock device being in the locked state and a second position relative to a lock core body which corresponds to the lock device being in the unlocked state.

Abstract: Pick quality determination is disclosed, including: using a pressure meter to sense a pressure associated with an airflow through a gripper mechanism of a diverting mechanism over time during a pick operation on a target object; storing the sensed pressure associated with the airflow through the gripper mechanism over time as a pressure sequence associated with the pick operation on the target object; and correlating the pressure sequence with representative pressure sequences associated with corresponding pick quality types to determine whether the pick operation on the target object was successful or not.

Abstract: Object picking optimization is disclosed, including: receiving an image of a target object; inputting the image in a machine learning model that is configured to output a pick probability heat map corresponding to the target object; selecting a pick location on the target object based at least in part on the pick probability heat map corresponding to the target object; and causing a diverting mechanism to perform a pick operation on the target object based at least in part on the pick location.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

Abstract: The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them. In some aspects, the disclosure provides mRNA wherein the uracil content has been modified and which may be particularly effective for use in therapeutic compositions, because they may benefit from both high expression levels and limited induction of the innate immune response. In some aspects, the disclosure provides methods for the production of pharmaceutical compositions including mRNA without reverse phase chromatography.

Abstract: The present invention is directed to a small push-type lawn mower that can be converted into a riding mower. The mower includes a platform at the rear of the mower deck, and its handle assembly that can be pivoted forward making room for a user to step onto the platform. The handle assembly includes controls that drive electric motors that power the rear wheels allowing the user to ride the mower. The handle assembly can be pivoted rearwardly so that the user can access and grab the handle assembly and push the mower as is well known.

Abstract: The disclosure provides polynucleotides encoding a polypeptide including a morpholino linker. In some embodiments, the polynucleotides of the invention have increased stability compared to wild-type polynucleotides.