Abstract: A chuck including a chuck body that supports a plurality of jaws. An outer sleeve is axially fixed with respect to the chuck body. A nut is coupled to the outer sleeve, and the nut is movable axially and radially relative to the chuck body. The nut interacts with the jaws such that when the outer sleeve rotates, the nut moves axially and radially relative to the body.

Abstract: A lawn mower comprising a frame supported on rotatable wheels for movement over a ground surface; an operator support coupled to the frame and configured to support the entire weight of an operator of the lawn mower during use thereof; a blade assembly comprising one or more blades that are configured to cut grass on the ground surface; a steering system configured to manipulate the steering direction of the wheels; a motor assembly configured to: drive the wheels so as to move the frame along the ground surface; and a first rechargeable battery module removably coupled to the frame and configured to power the motor assembly.

Abstract: A lawn mower includes a frame, an operator support, a blade assembly, a steering system, a motor assembly, a sensor and one or more processors. The operator support is coupled to the frame and is configured to support the entire weight of an operator of the lawn mower during use thereof. The motor assembly is configured to: drive rotatable wheels so as to move the frame along the ground surface; and drive at least one blade relative to the ground surface to cut grass. The sensor is configured to detect the presence of a person or an animal in a predetermined area proximate the lawn mower. The one or more processors are configured to: receive input from the sensor, and control the motor assembly stop driving the at least one blade, the wheels, or both based on the input from the sensor.

Abstract: The application describes methods for synthetic synthesis and cell-free synthesis of DNA vectors, particularly closed-ended DNA vectors (e.g., ceDNA vectors) having linear and continuous structure for delivery and expression of a transgene. The present invention relates to an in vitro process for production of closed-ended DNA vectors, corresponding DNA vector products produced by the methods and uses thereof, and oligonucleotides and kits useful in the process of the invention. DNA vectors produced using the methods described herein are free from unwanted side effects due to contaminants introduced during production in cell lines, for example, bacterial or insect cell lines. Further provided herein are methods and cell lines for reliable gene expression in vitro, ex vivo and in vivo using the ceDNA vectors synthesized using the methods herein.

Abstract: A chuck including a chuck body that supports a plurality of jaws. An outer sleeve is axially fixed with respect to the chuck body. A nut is coupled to the outer sleeve, and the nut is movable axially and radially relative to the chuck body. The nut interacts with the jaws such that when the outer sleeve rotates, the nut moves axially and radially relative to the body.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the cap analogs.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the cap analogs.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

Abstract: The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them.

Abstract: The present disclosure provides alternative sugar moieties and polynucleotides comprising such sugar moieties, and methods of use thereof.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

Abstract: An interchangeable electro-mechanical lock core for use with a lock device having a locked state and an unlocked state is disclosed. The interchangeable electromechanical lock core may include a moveable plug having a first position relative to a lock core body which corresponds to the lock device being in the locked state and a second position relative to a lock core body which corresponds to the lock device being in the unlocked state.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.

Abstract: The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them.

Abstract: The present disclosure provides alternative nucleosides, nucleotides, and nucleic acids, and methods of using them. In some aspects, the disclosure provides mRNA wherein the uracil content has been modified and which may be particularly effective for use in therapeutic compositions, because they may benefit from both high expression levels and limited induction of the innate immune response. In some aspects, the disclosure provides methods for the production of pharmaceutical compositions including mRNA without reverse phase chromatography.

Abstract: A lawn mower comprising a frame supported on rotatable wheels for movement over a ground surface; an operator support coupled to the frame and configured to support the entire weight of an operator of the lawn mower during use thereof; a blade assembly comprising one or more blades that are configured to cut grass on the ground surface; a steering system configured to manipulate the steering direction of the wheels; a motor assembly configured to: drive the wheels so as to move the frame along the ground surface; and a first rechargeable battery module removably coupled to the frame and configured to power the motor assembly.

Abstract: The present invention is directed to a small push-type lawn mower that can be converted into a riding mower. The mower includes a platform at the rear of the mower deck, and its handle assembly that can be pivoted forward making room for a user to step onto the platform. The handle assembly includes controls that drive electric motors that power the rear wheels allowing the user to ride the mower. The handle assembly can be pivoted rearwardly so that the user can access and grab the handle assembly and push the mower as is well known.

Abstract: The disclosure provides polynucleotides encoding a polypeptide including a morpholino linker. In some embodiments, the polynucleotides of the invention have increased stability compared to wild-type polynucleotides.

Abstract: A computer-implemented method includes accessing a plurality of sets of outputs for an interactive animation, with each set of outputs being associated with a different sequence of a plurality of sequences of discrete control inputs, and with each set of outputs comprising an output that provides a stored portion of the animation; and transmitting, to a client device, information indicative of at least one of the plurality of sets of outputs for the animation and the output that provides the stored portion of the animation, which when rendered by the client device causes the animation to be presented to a user.