Abstract: Provided herein are nucleic acid constructs, host insect cells, and methods for producing recombinant AAV capsids with high potency at high yield.

Abstract: Disclosed is a platform that manages worker users in a temporary staffing environment via an AI machine learning model. The machine learning model predicts dispatch outcomes of a plurality of pairings of worker users to potential shifts. A dispatch outcome predicts whether a worker will show up for and work a given shift. The machine learning model is based on a set of training data surrounding historical dispatch outcomes. The data surrounding the historical dispatch outcomes includes data relating to users, data relating to shifts, and data derived from a combination of both. An implementation of the machine learning model stitches together multiple shifts for up to a schedule horizon based on predicted dispatch outcomes.

Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device includes an optical assembly, a sample stage, and a receiver. The optical assembly enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume toward the receiver. A laser focus detection unit including at least one reference laser and photodetector generates at least one real-time detection signal indicative of one or more characteristics of the sample during ablation and/or of a distance from the objective to the sample stage or a surface of the sample. A controller coupled with the laser focus detection unit dynamically controls in real-time one or more parameters of the ablation laser and/or a position of the objective and/or a position of the receiver relative to the sample to improve MS imaging quality.

Abstract: Disclosed herein are systems for imaging and ablating a sample. An imaging/ablating device (110) includes an optical assembly (112), a sample stage (114), and a receiver (116). The optical assembly (112) is disposed in an inverted position below the sample stage (114) and the receiver (116) is positioned above the sample stage (112). The optical assembly enables imaging of a sample disposed on the sample stage (114). The optical assembly (112) also enables ablation of a region of interest within the sample. The laser light propagated from the optical assembly during ablation propagates substantially in the same direction as the direction of travel of the ablation plume (20) toward the receiver (116).

Abstract: A method for encapsulating transaction instrument information in a transaction includes storing, in association with a first transaction and by a processor of the primary transaction processor server, transaction data associated with a transaction instrument for a first transaction. The primary transaction processor server transmits the transaction data to a payment network processor, and receives an authorization package in response that includes a cryptograph and a first network token. The primary transaction processor server generates a second token that includes a changeable alpha-numeric string associated with the transaction instrument used in the first transaction, and transmits the second token to a merchant device. The second token indicates that a transaction associated with the second token is either i) a customer-initiated transaction or ii) a merchant-initiated transaction. The secondary transaction processor may approve or reject a transaction using the second token without a cryptogram.

Abstract: Provided are, inter alia, flow centration components that can be used in flow cytometers and other applications. A flow centrator can define central axis, a proximal end, and a distal end, and having a central bore extending within the flow centration component in the direction of the central axis; the flow centration component defining a splined outer surface that defines a plurality of circumferentially arranged bypass flute channels, the plurality of bypass flute channels extending in the direction of the central axis, and each of the bypass flute channels having a depth and a length. Also provided are related methods that utilize the disclosed components.

Abstract: The present disclosure relates to a fiber alignment device including a guide feature defining a fiber alignment groove. The fiber alignment device also includes a fiber engagement component defining a reference surface arrangement and elastic cantilever arms for pressing optical fibers into the fiber alignment groove. First portions of the guide feature engage the reference surface arrangement. Second portions of the guide feature engage the cantilever arms to flex the cantilever arms to a staged position.

Abstract: A system for connecting a fiber optic connector to a fiber optic adapter includes various alternative improvements, including improvements to the shutter, the alignment device, and the adapter in general.

Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a filamentous fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for modifying or altering the target site. Aspects in which the filamentous fungal cell being modified has a defective non-homologous end joining pathway are also provided.

Abstract: The present disclosure relates to fiber optic connection systems including fiber optic connector having retractable noses for protecting bare fiber ends of ferrule-less connectors. In certain examples, the retractable noses are used in combination with protective shutters. In other examples, the retractable noses can accommodate multiple optical fibers.

Abstract: An audio processing unit (APU) is disclosed. The APU includes a buffer memory configured to store at least one frame of an encoded audio bitstream, where the encoded audio bitstream includes audio data and a metadata container. The metadata container includes a header and one or more metadata payloads after the header. The one or more metadata payloads include dynamic range compression (DRC) metadata, and the DRC metadata is or includes profile metadata indicative of whether the DRC metadata includes dynamic range compression (DRC) control values for use in performing dynamic range compression in accordance with at least one compression profile on audio content indicated by at least one block of the audio data.

Abstract: Compositions and methods are provided for genome modification at a target site in the genome of a fungal cell. The methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for promoting modification of the DNA sequence at a target site in a fungal host cell genome.

Abstract: Methods of treating Alzheimer's Disease (AD) in patients suffering from mild to moderate AD, including ApoE4 positive patients and patients suffering from mild AD are provided.

Abstract: The present disclosure relates to a fiber alignment device (20) including a guide feature (62a, b) defining a fiber alignment groove (48). The fiber alignment device (20) also includes a fiber engagement component (44) defining a reference surface arrangement and elastic cantilever arms (56a, b) for pressing optical fibers into the fiber alignment groove (48). First portions of the guide feature (62a, b) engage the reference surface arrangement. Second portions of the guide (62a, b) feature engage the cantilever arms (56a, b) to flex the cantilever arms to a staged position.

Abstract: A system for connecting a fiber optic connector to a fiber optic adapter includes various alternative improvements, including improvements to the shutter, the alignment device, and the adapter in general.

Abstract: Systems and methods for generating tree-based models with improved fairness are disclosed. The disclosed process generates a first tree-based machine learning model, which is preferably trained to predict if a financial loan will be repaid. The process also determines an accuracy of the first tree-based machine learning mode. In addition, the process determines a fairness of the first tree-based machine learning model. The fairness is preferably associated with at least one of gender, race, ethnicity, age, marital status, military status, sexual orientation, and disability status. The process then generates a second different tree-based machine learning model, which is preferably trained based on the accuracy of the first tree-based machine learning model and the fairness of the first tree-based machine learning model. The process then combines the first tree-based machine learning model and the second tree-based machine learning model to produce a gradient-boosted machine learning model.

Abstract: Certain embodiments of the disclosure are directed to variant filamentous fungal cells, compositions thereof and methods thereof for increased production of one or more proteins of interest. More particularly, in certain embodiments, the disclosure is directed to variant filamentous fungal (host) cells derived from parental filamentous fungal cells, wherein the variant host cells comprise a genetic modification which enables the expression/production of a protein of interest (POI) in the absence of inducing substrate. In certain embodiments, a variant fungal host cell of the disclosure comprises a genetic modification which increases the expression of a variant activator of cellulase expression 3 (ace3) gene encoding an Ace3 protein referred to herein as Ace3-L.

Abstract: Tools and methods for creating a plurality of aligned holes from a plurality of near full-sized holes. The tool includes a cutting member having a first diameter and a pilot member connected to and coaxial with the cutting member. A first section of the pilot member includes a centering member and is positioned between the cutting member and the second section having a second diameter. The centering member may include a cutting surface. The second diameter corresponds to a positional tolerance of misalignment of a plurality of near full-sized holes of the same diameter and a full-size hole diameter. The centering member may have a third diameter between the first and second diameters. The centering member may be tapered. The pilot member may be inserted into a plurality of near-full sized holes and the cutting member may cut through the holes to form a plurality of aligned full sized holes.

Abstract: A method for trusted notifications comprises: receiving, at a first host having at least one trusted server, a request message from a sender computing device, the request message comprising a request identifier and instructions to: update, at a second host, a recipient account associated with a recipient computing device, and to make a corresponding update at the first host to a sender account associated with the sender computing device. The method further comprises authorizing, at the first host, the request message; updating the sender account; generating a secure message at the at least one trusted server, the secure message comprising the request identifier and an indication from the at least one trusted server that the update completed; and sending the secure message from the first host to the sender computing device. The secure message can be received at the sender computer device and conveyed to the recipient computing device.

Abstract: A sterile enclosure to bring a non-sterile hand-held electronic device such as a smartphone or a tablet into a sterile field comprises a rigid frame, a rigid base and a device holder configured to accept the hand-held device thereon. The use of the device holder to contain the hand-held device prior to engaging with the base and positioning the device therein, allows avoiding contamination of sterile components during use. A sterility indicator is also provided to continuously monitor the sterile enclosure for a possible breach in sterility while inside the sterile field.