Abstract: A method for controlling a parking mode for a vehicle includes detecting an activation action of a user of the vehicle, determining a movement status of the vehicle, and activating a parking mode for the vehicle by closing a parking brake of a brake device of the vehicle and by engaging a gearshift lock of a transmission of the vehicle.

Abstract: A method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and to the use thereof for producing amino acids and nucleotide. Genes coding for feedback-inhibited enzymes are mutagenized in vitro, —ligated into vectors in a further step—the vectors are each transformed in a microorganism containing a metabolite sensor in a further step, which brings about the synthesis of a fluorescent protein at an increased metabolite concentration, —whereupon microorganisms exhibiting increased or maximum fluorescence are selected; and —the vectors that contain a gene coding for an enzyme having reduced or deactivated feedback inhibition are isolated from microorganisms having increased or maximum fluorescence.

Abstract: An arrangement (1) of an electroacoustic actuator (2) on a vehicle structure (3) of a motor vehicle, has a housing (4), in which at least one loudspeaker (7) is arranged, which separates a front volume (8) from a rear volume (9) in the housing (4). The actuator (2) has a sound emission pipe (5), which projects outwards from the housing (4) and which is fluidically coupled with the front volume (8). A reduced introduction of noise into the vehicle arises when the actuator (2) is held on the vehicle structure (3) with a plurality of holding devices (19), which are arranged on the housing (4) in a circumferential direction (20) of the housing (4) spaced apart from one another. Each holding device (19) is designed as springy in a holding direction (21) and as rigid transversely thereto.

Abstract: A brake disk device for a vehicle having an annular brake disk with an encircling outer portion and an encircling inner portion, the inner diameter of which bounds a disk opening, and a brake pot with an annularly encircling edge portion, wherein the brake pot is fastened at the edge portion to the inner portion of the brake disk, wherein the edge portion is of multi-layered design at least in sections and/or is of thickened design in sections, wherein the walls of the edge portion are at least partially in contact, and an inner surface of the edge portion is designed as an additional brake surface.

Abstract: A method for identifying a cell having an intracellular concentration of a particular metabolite that is increased compared to the wild type of the cell, wherein the modification of the cell is achieved by recombineering, and to a method for producing a production cell that is genetically modified compared to the wild type of the cell and has optimized production of a particular metabolite, to a method for producing this metabolite, and to nucleic acids suited therefor. A gene coding for a recombinase, which is homologous to a known recombinase gene, is transformed in a cell using a vector, and a DNA containing at least one modified gene G1 to Gn, or at least one mutation M1 to Mn, is inserted into the cell, and the cell that has highest metabolite production is identified by way of metabolite sensors.

Abstract: The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for an autofluorescent protein, wherein the expression of the autofluorescent protein depends on the intracellular concentration of a particular metabolite. The present invention also relates to a method for the identification of a cell having an increased intracellular concentration of a particular metabolite, a method for the production of a cell which is genetically modified with respect to its wild type with optimized production of a particular metabolite, a cell obtained by this method, a method for the production of metabolites and a method for the preparation of a mixture.

Abstract: A pneumatic cooling device including a housing having a first end and a second end. A handle extends from an outer surface of the housing. An inlet is positioned at substantially the first end of the housing and is configured to receive a supply of medical grade air into the housing. An outlet is positioned at substantially the second end of the housing and is configured to deliver the supply of medical grade air outside of the housing. A pathway extends through the housing between the inlet and the outlet and is configured to passively move the supply of medical grade air between the inlet and the outlet.

Abstract: The present invention relates to an NADP(H) nanosensor comprising i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Abstract: A method for producing vectors containing a gene coding for an enzyme having reduced or deactivated feedback inhibition and to the use thereof for producing amino acids and nucleotide. Genes coding for feedback-inhibited enzymes are mutagenized in vitro, —ligated into vectors in a further step—the vectors are each transformed in a microorganism containing a metabolite sensor in a further step, which brings about the synthesis of a fluorescent protein at an increased metabolite concentration, —whereupon microorganisms exhibiting increased or maximum fluorescence are selected; and —the vectors that contain a gene coding for an enzyme having reduced or deactivated feedback inhibition are isolated from microorganisms having increased or maximum fluorescence.

Abstract: An arrangement (1) of an electroacoustic actuator (2) on a vehicle structure (3) of a motor vehicle, has a housing (4), in which at least one loudspeaker (7) is arranged, which separates a front volume (8) from a rear volume (9) in the housing (4). The actuator (2) has a sound emission pipe (5), which projects outwards from the housing (4) and which is fluidically coupled with the front volume (8). A reduced introduction of noise into the vehicle arises when the actuator (2) is held on the vehicle structure (3) with a plurality of holding devices (19), which are arranged on the housing (4) in a circumferential direction (20) of the housing (4) spaced apart from one another. Each holding device (19) is designed as springy in a holding direction (21) and as rigid transversely thereto.

Abstract: The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for an autofluorescent protein, wherein the expression of the autofluorescent protein depends on the intracellular concentration of a particular metabolite. The present invention also relates to a method for the identification of a cell having an increased intracellular concentration of a particular metabolite, a method for the production of a cell which is genetically modified with respect to its wild type with optimized production of a particular metabolite, a cell obtained by this method, a method for the production of metabolites and a method for the preparation of a mixture.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Abstract: An embossing method and apparatus (20) comprises a rotary embossing device (32) having a radial direction (28) and an outer peripheral surface (34). The embossing device (32) includes at least one embossing-component (48) which extends at least radially outward from the peripheral surface (34), and is configured to provide for a first embossing-pattern (38). The embossing device (32) also includes a rotary shaft member (46). A base embossing-segment (50) is operatively joined to the rotary shaft member (46) and configured to carry a first base-section (40) of the first embossing-component (48). In a particular aspect, a first, supplemental embossing-segment (52) is operatively joined and selectively positionable on the rotary shaft member (46), and is configured to carry a first supplemental-section (42) of the first embossing-component (48).

Abstract: An apparatus and process (20) for forming an embossed web or other article can include moving a target, composite web (26) along an appointed machine-direction (22) at a selected web speed, and operatively contacting the target web (26) with a rotary embossing device (38) to thereby form a non-linear embossment region (82) in at least a selected embossment portion of the target web (26). For example, the web speed can be at least a minimum of about 1.9 m/sec. In a particular aspect, the embossing device (38) can include an outer peripheral surface (42) having a lateral cross-direction (24) and a circumferential-direction (40), and can include a non-linear embossing member (44) located on the outer surface (42). In another aspect, the embossing member (44) can be configured to include a selected traversing occurrence along the circumferential-direction (40) of the embossing device (38). In a further aspect, the embossing member (44) can be configured to include a selected lateral traversing distance (50).

Abstract: Process for the production of a protein with citrate lyase activity by expressing a suitable plasmid in a host organism and isolating the protein in an active form, wherein the plasmid contains the information from a gene cluster composed of at least six genes and an inducible promoter. Furthermore the invention concerns the use of the recombinant enzyme and a corresponding test kit for the determination of citric acid.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.

Abstract: The invention relates to a method for the microbial production of metabolic products, polynucleotides from coryne-form bacteria and use thereof. According to the invention, by means of said method and polynucleotides it is possible to influence the synthesis of ATP in a controlled manner and also to control the synthesis of metabolic products. The invention relates to genes from Corynebacterium glutamicum coding for cytochrome aa3 oxidase and the cytochrome bc1 complex. The monocistronic ctaD gene codes for a 65 kDa protein, the primary structure of which displays all the typical properties of the sub-unit I of cytochrome aa3 oxidase. The genes which code for the sub-unit III of the cytochrome aa3 (ctaE) and the three characteristic sub-units of the cytochrome bc1 complex (qcrABC) are arranged in a group with the sequence ctaE-qcrCAB.

Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the MtrA and/or MtrB proteins and processes for the fermentative preparation of amino acids using bacteria in which the mtrA and/or mtrB genes are attenuated.