Abstract: The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.

Abstract: Methods and compositions for treating for the synergistic treatment of fungal associated disorders are discussed.

Abstract: An apparatus and method are provided for insulating a seal (110) made of a polymeric material in a pressurized, evacuated or exhausted process chamber (100) that reduces damage due to heat transferred to the seal from the chamber. The chamber (100) includes a wall (125) having an aperture (140) therein, a flange (115) disposed about the aperture, and an insulator (105) between the flange and a fixture (120) to insulate the seal between the flange and the fixture. Generally, the insulator (105) includes a cooling tube or loop (260) having a sidewall (262) overlying and attached to the flange (115), and a cap (255) overlying the loop. The cap (255) has a sealing surface (257) against which the seal (110) seats. Fluid may be passed through the loop (260) to reduce heat transferred to the seal (110). Preferably, the flange (115), the loop (260), and the cap (255), are made of quartz or a glass, and the sidewall (262) of the loop is welded to the flange and the cap.

Abstract: Methods and compositions for treating fungal associated disorders in subjects are discussed.