Abstract: The present invention relates to a method of determining an analyte in a sample, said method comprising (a) contacting said sample with (i) a binding compound binding to said analyte, said binding compound comprising a binding agent and a first partner of an affinity pair (first affinity partner); and (ii) a second partner of the affinity pair (second affinity partner) coupled to a solid surface, to an indicator, and/or to a second binding agent; and (b) determining said analyte based on complexes formed in step (a); wherein one of said first affinity partner and said second affinity partner is a polypeptide comprising the amino acid sequence of SEQ ID NO:1 or a sequence at least 50% identical thereto, and wherein the other of said first affinity partner and said second affinity partner is a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a sequence at least 50% identical thereto.

Abstract: A probe head with a cylindrical portion and an oblique truncated portion angled downward toward a high-powered electronic device under testing. The probe head has a proximal end comprising an oblique truncated cone portion angled relative to the longitudinal axis at an input angle and has an analog signal input. The probe head has a digital signal output provided on the distal end of the housing.

Abstract: The invention relates to a method for separating a polynucleotide insert from a polynucleotide vector backbone. The backbone has a plurality of cleavage sites distributed such that the backbone is converted into fragments when the sites are cleaved. This allows straightforward separation of the insert from the backbone. The invention also relates to backbones for use in such a method, and to plasmids and kits comprising such backbones.

Abstract: A two-stroke engine possesses a cylinder, in the cylinder bore of which a combustion chamber is formed. The combustion chamber is bounded by a reciprocating piston, which drives a crankshaft rotatably mounted in a crankcase. A crankcase interior is fluidically connected to the combustion chamber across at least one transfer channel in at least one position of the piston. The at least one transfer channel emerges with a discharge opening in the crankcase interior and with at least one transfer window on the cylinder bore. It is proposed that all transfer channels have an average length as measured from the discharge opening to the transfer window, the average length being at least 1.5 in relation to the stroke, and that the volume of the crankcase interior including all transfer channels is at most 3.1 in relation to the piston displacement.

Abstract: A method is provided for determining a starting point between entities. The locations of the entities are determined in a map, a circle enclosing all entities is determined and an optimum position of the starting point is determined iteratively. The starting point initially coincides with a center point of the circle enclosing the entities. To find an optimum position of the starting point for each entity, a route is determined between the respective location and the starting point. In order to shift the position of the starting point, a sum of all of the temporal or physical distances of the routes between entities and the starting point falls below a defined maximum distance, and/or a temporal or physical distance difference between a shortest and a longest route falls below a specified maximum difference.

Abstract: A circuit configuration includes a voltage surge protector that is connected in parallel to the link capacitor to protect the link capacitor from voltage overloads. A printed circuit board assembly, an electric axle drive, and a motor vehicle are also disclosed.

Abstract: Disclosed is parallel hybrid drive for a motor vehicle, a motor vehicle, a method for operating a parallel hybrid drive in an all-electric mode, a method for operating a parallel hybrid drive in a direct drive mode, and a method for operating a parallel hybrid drive in a CVT mode. The drive for a motor vehicle includes an electric machine operable as a motor and generator, an internal combustion engine, a drive axle, and an epicyclic gear. The epicyclic gear includes: a first shaft connected to the electric machine; a second shaft connected to the internal combustion engine; and a third shaft connected to the drive axle. A clutch element is configured to firmly connect at least two shafts of the epicyclic gear to each other. A first brake element is configured to prevent rotation of the internal combustion engine in one direction of rotation.

Abstract: Provided are embodiments for a computer-implemented method, system, and device for tracking multiphase flow in a microfluidic device. Embodiments include receiving first readings from a first sensor of the microfluidic device, the first reading representing a detection of a fluid at an interface between the fluid and the first sensor, and receiving second readings from a second sensor of the microfluidic device, the second readings representing a detection of the fluid at an interface between the fluid and the second sensor, wherein the first sensor is located at a distance from the second sensor. Embodiments also include calculating a flow speed of the fluid in the microfluidic device based at least in part on a difference of time between the detections by the first sensor and the second sensor, and the distance between the first sensor and the second sensor.

Abstract: A temperature-independent optical link for converting a received electrical signal to an analog signal. The temperature-independent optical link comprises a temperature-controlled transmitter chamber housing an ETO transmitter and a feedback-loop temperature control system. The optical link is housed in a probe head having a power supply device, and a probe tip. The temperature-independent optical link is used in a method for converting a received electrical signal to an analog signal.

Abstract: A method is provided for machine vision and image analysis for recognizing an object in an electronic image, which is captured with the aid of an optical sensor. A reference image of the object to be recognized is trained during a learning phase and compared with the image of the scene during a working phase, the pattern comparison between the object and the scene takes place with the aid of a modified census transform, using a determination of maximum and which must exceed a threshold value for a positive statement on a degree of correspondence.

Abstract: In a self-propelled construction machine (1), in particular slipform paving machine, comprising a machine frame (2), at least three travelling devices (14), wherein the travelling devices (14) are each connected to the machine frame (2) by means of lifting columns (12), wherein at least two of the at least three travelling devices (14) are steerable by means of one each steering drive (6), wherein the respective steering drive (6) comprises at least one first part (10), which is connected to the respective travelling device (14), and one second part (16), which is connected to the respective lifting column (12), it is provided that the steering drive (6) comprises at least one first toothed rack (22), wherein the first toothed rack is movable by means of at least one first linear drive means (26), wherein teeth of the at least one toothed rack engage with teeth of at least one toothed wheel (17), and the toothed wheel is thus rotatable by means of the movement of the toothed rack.

Abstract: Evaluating enhanced oil recovery methods by identifying a first nanofluidic device associated with a reservoir rock formation and saturated with a target fluid, directing the injection of a secondary recovery fluid into the first nanofluidic device, determining a target fluid secondary recovery saturation level associated with injection of the secondary recovery fluid into the first nanofluidic device, identifying a second nanofluidic device associated with the reservoir rock formation and saturated with the target fluid, directing the injection of a tertiary recovery fluid into the second nanofluidic device, determining a target fluid tertiary recovery saturation level associated with the injection of the tertiary recovery fluid into the second nanofluidic device, determining a target fluid production efficiency associated with the tertiary recovery fluid, and providing the target fluid production efficiency to a user.

Abstract: A slipform paving machine includes an offset mold, including a mold frame, a form insert, a form insert actuator and a form insert sensor. A controller receives a signal from an external reference sensor and controls a position of the form insert actuator to control the height of the form insert relative to the mold frame and thereby control a height of at least a portion of a molded structure relative to a ground surface at least in part in response to the signal from the external reference sensor.

Abstract: An adjustable width mold apparatus for a slipform paver includes a center portion and left and right sideform assemblies. The center portion has left and right lateral ends. Left and right adjustable width support assemblies are connected between the sideform assemblies and the center portion. One or more spacers may be received between each sideform assembly and the center portion to adjust the width of the mold apparatus. The spacers may be hung on a plurality of hanger rods. Each of the hanger rods may have a hydraulic nut on one end thereof for clamping the spacers between the sideform assembly and the center portion.

Abstract: Systems and methods for implementing an automation platform that is configured to analyze computing activities from a plurality of users so as to identify potential automation processes is provided. In one or more examples, a plurality of data collection agents are deployed across a plurality of computing devices and can be configured to collect and record activities performed on the computing device by one or more users of the computing devices. In one or more examples, each agent deployed on a computing device can be configured to transmit the collected data to a central server that can store the collected data in memory. The central server can be configured to collect the data from each agent and can be configured to apply one or more data science algorithms that can be configured to cluster various activities collected by the agents into groups for potential automation.

Abstract: Evaluating enhanced oil recovery methods by identifying a first nanofluidic device associated with a reservoir rock formation and saturated with a target fluid, directing the injection of a secondary recovery fluid into the first nanofluidic device, determining a target fluid secondary recovery saturation level associated with injection of the secondary recovery fluid into the first nanofluidic device, identifying a second nanofluidic device associated with the reservoir rock formation and saturated with the target fluid, directing the injection of a tertiary recovery fluid into the second nanofluidic device, determining a target fluid tertiary recovery saturation level associated with the injection of the tertiary recovery fluid into the second nanofluidic device, determining a target fluid production efficiency associated with the tertiary recovery fluid, and providing the target fluid production efficiency to a user.

Abstract: Aspects of the invention include determining, by a first AFM tip, a first snap-off force of a solid surface immersed in a first fluid, determining, by a second AFM tip, a second snap-off force, determining, by a third AFM tip, a third snap-off force, determining, by the first AFM tip, a fourth snap-off force of a droplet of the first fluid immersed in the second fluid on the solid surface, determining, by the second AFM tip, a fifth snap-off force, determining, by the third AFM tip, a sixth snap-off force, determining a first capillary force for first AFM tip and first droplet based on first snap-off force and fourth snap-off force, determining a second capillary force for second AFM tip and first droplet and a third capillary force for third AFM tip and first droplet, and determining interfacial tension between first fluid and second fluid based on the capillary forces.

Abstract: Techniques for determining flow properties of a fluid in a fluidic device comprising a light source configured to generate a plurality of optical signals, tracers suspended in a fluid, a plurality of photonic devices, each including a photonic element and flow channel, and a measurement device configured to: determine a first measurement based on the plurality of optical signals and the tracers in a flow channel of a first photonic device of the plurality of photonic devices, determine a second measurement based on the plurality of optical signals and the tracers in a flow channel of a second photonic device of the plurality of photonic devices, and determine a property associated with a flow of the fluid or the tracers based on the first measurement and the second measurement.

Abstract: Provided are embodiments for a computer-implemented method, system, and device for tracking multiphase flow in a microfluidic device. Embodiments include receiving first readings from a first sensor of the microfluidic device, the first reading representing a detection of a fluid at an interface between the fluid and the first sensor, and receiving second readings from a second sensor of the microfluidic device, the second readings representing a detection of the fluid at an interface between the fluid and the second sensor, wherein the first sensor is located at a distance from the second sensor. Embodiments also include calculating a flow speed of the fluid in the microfluidic device based at least in part on a difference of time between the detections by the first sensor and the second sensor, and the distance between the first sensor and the second sensor.

Abstract: Aspects of the invention include determining, by a first AFM tip, a first snap-off force of a solid surface immersed in a first fluid, determining, by a second AFM tip, a second snap-off force, determining, by a third AFM tip, a third snap-off force, determining, by the first AFM tip, a fourth snap-off force of a droplet of the first fluid immersed in the second fluid on the solid surface, determining, by the second AFM tip, a fifth snap-off force, determining, by the third AFM tip, a sixth snap-off force, determining a first capillary force for first AFM tip and first droplet based on first snap-off force and fourth snap-off force, determining a second capillary force for second AFM tip and first droplet and a third capillary force for third AFM tip and first droplet, and determining interfacial tension between first fluid and second fluid based on the capillary forces.