Abstract: Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

Abstract: A dolly storage system for storing a dolly in a bed of a pickup truck including one or more slidable tubes positioned within an interior of a truck bed; a dolly; a mounting system connected to the one or more slidable tubes, the mounting system configured to connect to the dolly; and an articulating arm coupling the mounting system to the dolly, wherein the one or more slidable tubes and the articulating arm are configured to enable movement of the dolly between a stowed configuration within the interior of the truck bed and an extended configuration outside of the truck bed.

Abstract: Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

Abstract: Systems and methods for attaching with a ski to provide improved traction while climbing snowy grades. Exemplary climbing track assemblies can include an attachment to the bottom of a ski that improves traction for hiking or climbing uphill. Assemblies can also include an under-binding bracket, which is mounted under the toe-piece binding of the ski which allows the device to be securely fastened. Assemblies may further include a shim, provided as a small plate mounted under the heel-piece binding, which operates to keep the binding level.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for implementing a policy layer for controlling how applications interact with internal or external machine learning models. One of the methods includes receiving an original input from an application. One or more input matching processes are performed to identify one or more matching input policy routines. One or more actions are performed according to the one or more matching input policy routines to generate a modified input. The modified input is provided instead of the original input to the machine learning system.

Abstract: The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.

Abstract: The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.

Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.

Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.

Abstract: Systems and methods for attaching with a ski to provide improved traction while climbing snowy grades. Exemplary climbing track assemblies can include an attachment to the bottom of a ski that improves traction for hiking or climbing uphill. Assemblies can also include an under-binding bracket, which is mounted under the toe-piece binding of the ski which allows the device to be securely fastened. Assemblies may further include a shim, provided as a small plate mounted under the heel-piece binding, which operates to keep the binding level.

Abstract: Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

Abstract: Systems and methods for attaching with a ski to provide improved traction while climbing snowy grades. Exemplary climbing track assemblies can include an attachment to the bottom of a ski that improves traction for hiking or climbing uphill. Assemblies can also include an under-binding bracket, which is mounted under the toe-piece binding of the ski which allows the device to be securely fastened. Assemblies may further include a shim, provided as a small plate mounted under the heel-piece binding, which operates to keep the binding level.

Abstract: Systems and methods for attaching with a ski to provide improved traction while climbing snowy grades. Exemplary climbing track assemblies can include an attachment to the bottom of a ski that improves traction for hiking or climbing uphill. Assemblies can also include an under-binding bracket, which is mounted under the toe-piece binding of the ski which allows the device to be securely fastened. Assemblies may further include a shim, provided as a small plate mounted under the heel-piece binding, which operates to keep the binding level.

Abstract: The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.

Abstract: The present disclosure is directed to epitope-tagged antibodies, as well as methods of employing the epitope-tagged antibodies for detecting one or more targets in a biological sample, e.g. a tissue sample.

Abstract: A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, athiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

Abstract: Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

Abstract: The techniques described herein relate to methods and apparatus for firearm safety mechanisms, visual safety indicators, and related techniques. A firearm includes a handle with one or more sensors disposed at least partially within a surface of the handle. The firearm also includes a trigger that is actuatable to fire the firearm, and a safety mechanism that has a first position that blocks actuation of the trigger and a second position that does not block the trigger. The firearm further includes at least one processor configured to receive data from the one or more sensors, process the received data to determine whether the data is indicative of sufficient contact of the operator's hand with the one or more sensors, and upon determining the data is indicative of sufficient contact, transmitting a signal to the safety mechanism to change the safety mechanism from the first position to the second position.

Abstract: Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.

Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.