Abstract: The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.

Abstract: A method for preparing a nucleic acid probe is provided. The method comprises forming an activated cytosine or cytidine by a bisulfite catalyzed reaction; and covalently linking a reporter molecule to the activated cytosine or cytidine, wherein said activating step, said covalently linking step, or both are conducted in the presence of microwave energy. Also provided by the invention are nucleic acid probes.

Abstract: Disclosed embodiments concern quantifying a biomolecule conjugated to a nanoparticle. Quantifying typically comprises determining the number of biomolecules per nanoparticle. Any suitable biomolecule can be used, including but not limited to, amino acids, peptides, proteins, haptens, nucleic acids, oligonucleotides, DNA, RNA, and combinations thereof. A single type of biomolecule may be conjugated to the nanoparticle, more than one biomolecule of a particular class may be conjugated to the nanoparticle, or two or more classes of biomolecules may be conjugated to the nanoparticle. Certain disclosed embodiments comprise enzymatically or chemically digesting a biomolecule conjugated to the nanoparticle, or displacing a biomolecule using ligand-exchange chemistry.

Abstract: A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

Abstract: The present disclosure provides nucleic acid probes, as well as kits that include such probes. Methods for producing and using (for example in chromosomal in situ hybridization) the probes are also provided. Such probes in some examples are used to detect chromosomal abnormalities or the presence of a pathogen.

Abstract: Method for predicting lithology and porosity of subsurface rocks from seismic reflection data. The seismic data is inverted to yield elastic properties of the rocks such as the compressional and shear impedances. A rock physics model is built to relate porosity, the shale volume fraction, the fluid content of the rock and the elastic properties of the rock. The model is run backward in a second inversion process to solve for porosity and lithologic properties such as the shale volume fraction.

Abstract: A container for use in a package. The container may be disposed inside of another container and a visible from outside of the package system. The container, may expand to appear full, or to touch the inside of the outer container in which it is disposed. This arrangement provides an aesthetically pleasing appearance if the container is visible from outside the package and/or for transmission of dynamic shock load.

Abstract: A method for determining a toner usage estimate for a print job to be performed by a printing system having at least one print engine and at least one controller includes determining a set of print conditions for the print job and selecting a set of parameters associated with the print conditions. The method further includes estimating a total amount of toner usage for each of the print job's pages. The set of parameters may include image rendering, job dependent and engine dependent parameters.

Abstract: Apparatus are provided, including a document application and a bleed creation mechanism. The bleed creation mechanism includes an image extender to automatically determine non-blank extended image information based on a location of a blank perimeter area of a page description language (PDL) file. The image extender further populates the blank perimeter area with the non-blank extended image information.

Abstract: A method is provided for managing a print job in a distributed printing system including a first printing system having a first default operating mode with a first rated output and a second printing system having a second default operating mode with a second rated output. The method includes determining that the second printing system has the capability to operate in an adjusted operating mode with an effective output that differs from either the first rated output or the second rated output. In practice the print job is split into first and second job portions in such a way that when (a) both the first and second print job portions are communicated to the distributed printing arrangement, (b) the first print job portion is printed at the first printing system in the first default operating mode, and (c) the second print job portion is printed at the second printing system in the adjusted operating mode, printing of the first and second print job portions end substantially simultaneously.

Abstract: A method for managing a print job in a distributed printing system is provided. The method includes: determining the first output rate of a first printing system and the second output rate of a second printing system. The print job is then split into a first print job portion having a first size and a second print job portion having a second size. The first and second sizes are respectively proportionate to the first and second output rates so that when (a) both the first and second print job portions are respectively communicated to the first and second printing systems, (b) the first print job portion is printed at the first output rate in the first printing system, and (b) the second print job portion is printed at the second output rate in the second printing system, printing of the first and second print job portions end substantially simultaneously.

Abstract: A document can contain multiple print layers. Different layers can be combined to produce different document versions. In this manner, a document can have an English version and a Spanish version that share graphics layers but have different text layers. A document's different layers can be difficult to track and the difficulty can lead to errors in document proofing and production. A discrimination module can automatically classify layers as common, graphics, and text containing. The text containing layers can be further grouped by native language. The layer groups can then be automatically combined to produce the desired document version specifications that can be rendered as document versions.

Abstract: A document can contain multiple print layers. Different layers can be combined to produce different document versions. In this manner, a document can have an English version and a Spanish version sharing graphics layers but having different text layers. A document version specification can be used for producing a certain version of the document. Some documents, however, have many layers and desired layer combinations. A set of document version specifications can be used to ensure that all the desired versions are produced without any version being forgotten or any undesired version being produced. Dividing the layers amongst a set of layer groups helps in creating the specifications. A set of rules can be submitted to a rule module to automatically generate the desired specifications.

Abstract: A method and composition are disclosed that are useful for processing biological samples. In one aspect, a biological sample such as a tissue section is treated using a histochemical technique and is contacted with a lipid compound during the process to enhance the definition of cellular and sub-cellular features that are observable in the sample when it is viewed microscopically. In other aspects, a coverslipping composition that includes a lipid compound and a method of coverslipping a sample using the coverslipping composition are disclosed.

Abstract: Disclosed are systems and methods enabling a user to select optimal parameters for a printing job. The systems and methods disclosed are directed to facilitating the adjustment of printing parameters based upon at least the knowledge of a particular aspect of a printing environment (e.g., printer) and further including the ability to dynamically adjust parameters in relation to dynamically adjustable parameters, wherein the parameters may be determined as a function of other specified parameters.

Abstract: A method for relating to each other cardiovascular and sleep disordered breathing conditions of a patient. The patient's heart rate and/or detailed echocardiogram data is monitored and recorded continuously or periodically together with sleep disordered breathing information on similar time scales. Changes in the patient's heart rate associated with changes in sleep disordered breathing can then be observed. More specifically, positive airway pressure at therapeutic levels for treatment of sleep disordered breathing is applied while events associated with the treatment of the patient's sleep disordered breathing are detected and recorded. At the same time, information concerning the cardiovascular condition of the patient is stored and the stored information concerning the cardiovascular condition of the patient and the recorded events associated with the treatment of the patient's sleep disordered breathing are related to each other.

Abstract: A method of planning print production in a print production enterprise, having multiple print shop equipment components performing multiple discrete printing operations, includes gathering print job data and populating the variables of a simulation algorithm with the print job data. The print job production run is planned utilizing the simulation algorithm and then implemented. Multiple workflow variables associated with the print job production run are measured and the variables of the simulation algorithm are conformed to the measured workflow variables.

Abstract: Signals propagating on an aggressor communication channel can cause detrimental interference in a victim communication channel. A signal processing circuit can generate an interference cancellation signal that, when applied to the victim communication channel, cancels the detrimental interference. The signal processing circuit can dynamically adjust or update two or more aspects of the interference cancellation signal, such as an amplitude or gain parameter and a phase or delay parameter. Via the dynamic adjustments, the signal processing circuit can adapt to changing conditions, thereby maintaining an acceptable level of interference cancellation in a fluctuating operating environment. A control circuit that implements the parametric adjustments can have at least two modes of operation, one for adjusting the amplitude parameter and one for adjusting the phase parameter. The modes can be selectable or can be intermittently available, for example.

Abstract: A method and system for determining chromatic content of a page of an electronic document. Object level color analysis is performed on a page of the electronic document to determine whether the page is chromatic, achromatic or has highlight color, based on the object level color analysis. The page is then classified as chromatic, achromatic or highlight color, based on the results of the determination.