Patents by Inventor Michael H. Wigler

Michael H. Wigler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170152548
    Abstract: The present invention, SMASH (Short Multiply Aggregated Sequence Homologies), is a technique designed to pack multiple independent mappings into every read. Specifically, the invention relates to a composition comprising a first mixture of different chimeric genomic nucleic acid fragments, wherein each different fragment in the mixture comprises randomly ligated DNA segments, wherein each DNA segment in the fragment is a nucleic acid molecule at least 27 base pairs in length resulting from random fragmentation of a single genome. The invention also relates to methods for generating said composition and use of said composition to obtain genomic information, for example, copy number variation.
    Type: Application
    Filed: January 30, 2017
    Publication date: June 1, 2017
    Applicant: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, Dan Levy, Zihua Wang
  • Patent number: 8694263
    Abstract: The invention provides oligonucleotide probes that can be used to hybridize to a representation of nucleic acid sequences. Compositions containing the probes such as microarrays are also provided. The invention also provides methods of using these probes and compositions in therapeutic, diagnostic, and research applications. Systems and methods for using a word counting algorithm that can quickly and accurately count the number of times a particular string of characters (i.e., nucleotides) appears in a nucleotide sequence (e.g., a genome) are provided. This algorithm can be used to identify the oligonucleotide probes of the invention. The algorithm uses a transform of a genome and an auxiliary data structure to count the number of times a particular word occurs in the genome.
    Type: Grant
    Filed: May 21, 2004
    Date of Patent: April 8, 2014
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H Wigler, John Healy, Robert Lucito
  • Publication number: 20140025307
    Abstract: The present invention provides methods and compositions related to genomic profiling, and in particular, to assigning probabilistic measure of clinical outcome for a patient having a disease or a tumor using segmented genomic profiles such as those produced by representational oligonucleotide microarray analysis (ROMA).
    Type: Application
    Filed: July 16, 2013
    Publication date: January 23, 2014
    Applicant: COLD SPRING HARBOR LABORATORY
    Inventors: Michael H. Wigler, James B. Hicks, Alexander Krasnitz, Anders P. Zetterberg
  • Patent number: 8554488
    Abstract: The present invention provides methods and compositions related to genomic profiling, and in particular, to assigning probabilistic measure of clinical outcome for a patient having a disease or a tumor using segmented genomic profiles such as those produced by representational oligonucleotide microarray analysis (ROMA).
    Type: Grant
    Filed: December 14, 2006
    Date of Patent: October 8, 2013
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, Lakshmi Muthuswamy, Jonathan Sebat
  • Publication number: 20100227768
    Abstract: The present invention provides methods and compositions related to genomic profiling, and in particular, to assigning probabilistic measure of clinical outcome for a patient having a disease or a tumor using segmented genomic profiles such as those produced by representational oligonucleotide microarray analysis (ROMA).
    Type: Application
    Filed: December 14, 2006
    Publication date: September 9, 2010
    Inventors: Michael H. Wigler, James B. Hicks, Alexander Krasnitz, Anders P. Zettenberg
  • Patent number: 6936477
    Abstract: Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detachable from the particle or retained on the particle.
    Type: Grant
    Filed: May 17, 2002
    Date of Patent: August 30, 2005
    Assignees: The Trustees of Columbia University in the City of New York, Cold Spring Harbor Laboratory
    Inventors: W. Clark Still, Michael H. J. Ohlmeyer, Lawrence W. Dillard, John C. Reader, Michael H. Wigler
  • Publication number: 20040096956
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Application
    Filed: October 20, 2003
    Publication date: May 20, 2004
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Publication number: 20040086900
    Abstract: A method of cloning mammalian genes encoding proteins which can function in microorganisms, particularly yeast, and can modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. It further relates to mammalian genes cloned by the present method, as well as to products encoded by such genes and antibodies which can bind the encoded proteins. More specifically, the present invention relates to a method of cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS protein, to products (RNA, proteins) enocded by the mammalian genes cloned in this manner and to antibodies which can bind the encoded proteins.
    Type: Application
    Filed: May 21, 2003
    Publication date: May 6, 2004
    Inventors: Michael H. Wigler, John J. Colicelli
  • Patent number: 6635424
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Grant
    Filed: March 2, 2001
    Date of Patent: October 21, 2003
    Assignee: Stratagene
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Publication number: 20030119059
    Abstract: Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle.
    Type: Application
    Filed: May 17, 2002
    Publication date: June 26, 2003
    Applicant: The Trustees of Columbia University in the City of New York
    Inventors: W. Clark Still, Michael H.J. Ohlmeyer, Lawrence W. Dillard, John C. Reader, Michael H. Wigler
  • Patent number: 6569617
    Abstract: A method of cloning mammalian genes encoding proteins which can function in microorganisms, particularly yeast, and can modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. It further relates to mammalian genes cloned by the present method, as well as to products encoded by such genes and antibodies-which can bind the encoded proteins. More specifically, the present invention relates to a method of cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner and to antibodies which can bind the encoded proteins.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: May 27, 2003
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, John J. Colicelli
  • Publication number: 20030054001
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies is described. In addition, a method of producing antigen-combining molecules which does not require an in vivo procedure is described. Vectors useful in the present method and antigen-combining molecules produced by the method are discussed. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Application
    Filed: March 2, 2001
    Publication date: March 20, 2003
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Patent number: 6503759
    Abstract: Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle.
    Type: Grant
    Filed: February 7, 1997
    Date of Patent: January 7, 2003
    Assignees: The Trustees of Columbia University in the City of New York, Cold Spring Harbor Laboratory
    Inventors: W. Clark Still, Michael H. J. Ohlmeyer, Lawrence W. Dillard, John C. Reader, Michael H. Wigler
  • Patent number: 6479243
    Abstract: Disclosed is an in vitro process for synthesizing DNA encoding a family of antigen-combining proteins. This process involves obtaining DNA containing genes encoding antigen-combining proteins and then combining these genes with sequence specific primers. These primers can be oligonucleotides homologous to conserved regions of the genes. The genes and primers are then subjected to sequence specific gene amplification.
    Type: Grant
    Filed: March 5, 2001
    Date of Patent: November 12, 2002
    Assignee: Stratagene
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Patent number: 6455275
    Abstract: The invention relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules which includes genes coding for desired proteinaceous materials. The insertion of multiple copies of desired genes is accomplished by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: September 24, 2002
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Richard Axel, Michael H. Wigler, Saul J. Silverstein
  • Patent number: 6303313
    Abstract: A method of producing libraries of genes encoding antigen-combining molecules or antibodies; a method of producing antigen-combining molecules which does not require an in vivo procedure; a method of obtaining antigen-combining molecules of selected specificity which does not require an in vivo procedure; vectors useful in the present method; and antigen-combining molecules produced by the method. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.
    Type: Grant
    Filed: November 12, 1999
    Date of Patent: October 16, 2001
    Assignee: Stratagene
    Inventors: Michael H. Wigler, Joseph A. Sorge
  • Patent number: 6100025
    Abstract: Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.
    Type: Grant
    Filed: March 1, 1994
    Date of Patent: August 8, 2000
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, John J. Colicelli
  • Patent number: 6080540
    Abstract: A method of cloning mammalian genes encoding proteins which can function in microorganisms, particularly yeast, and can modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. It further relates to mammalian genes cloned by the present method, as well as to products encoded by such genes and antibodies which can bind the encoded proteins. More specifically, the present invention relates to a method of cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner and to antibodies which can bind the encoded proteins.
    Type: Grant
    Filed: April 20, 1990
    Date of Patent: June 27, 2000
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Michael H. Wigler, John J. Colicelli
  • Patent number: 6069240
    Abstract: Disclosed are methods for detecting mammalian genes encoding proteins which can function in microorganisms, particularly yeast, to modify, complement, or suppress a genetic defect associated with an identifiable phenotypic alteration or characteristic in the microorganism. Disclosed also are mammalian DNA sequences cloned by the above method, as well as polypeptide products of the expression of the DNA sequences in procaryotic or eucaryotic host cells and antibody substances which are specifically immunoreactive with said expression products. More specifically, the present invention relates to methods for cloning mammalian genes which encode products which modify, complement or suppress a genetic defect in a biochemical pathway in which cAMP participates or in a biochemical pathway which is controlled, directly or indirectly, by a RAS-related protein, to products (RNA, proteins) encoded by the mammalian genes cloned in this manner, and to antibodies which can bind the encoded proteins.
    Type: Grant
    Filed: September 3, 1998
    Date of Patent: May 30, 2000
    Assignee: ICOS Corporation
    Inventors: Michael H. Wigler, John J. Colicelli
  • Patent number: 6001579
    Abstract: Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: December 14, 1999
    Assignees: The Trustees of Columbia University, Cold Spring Harbor Laboratory
    Inventors: W. Clark Still, Michael H. Wigler, Michael H. J. Ohlmeyer, Lawrence W. Dillard, John C. Reader