Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3? modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: A method for determining a trust indicator is provided. The method may include interpreting, via an Internet of Things Universal Identifier (IoT UID) processing circuit, an IoT UID corresponding to a device and identifying, via a record management circuit and based at least in part on the IoT UID, a record in a database corresponding to the device. The method may further include determining, via a trust analysis circuit and based at least in part on the record, a risk indicator of the device and transmitting, via an indicator provisioning circuit, the risk indicator.

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3? modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: Example embodiment relate to an air conditioner. A fastening device fastens a compressor on a bottom of a housing via a mounting component. A clamping element is arranged within a guiding element. The guiding element is arranged in a recess of the bottom of the housing and has a smaller length than the recess. Two stop elements are each associated with a front side of the clamping element, an axial distance between the stop elements being variable in the unassembled state and being fixed in an assembled state.

Abstract: The present disclosure provides nucleotide conjugates each configured to include a core attached to multiple nucleotide-arms, where the nucleotide-arms are modular and comprise (i) a core attachment moiety, (ii) a spacer, (iii) a linker, and (iv) a nucleotide unit. The nucleotide unit of each nucleotide-arm can bind a polymerase which is complexed with a nucleic acid template and nucleic acid primer. The nucleotide unit can bind the 3? end of the primer at a position that is opposite a complementary nucleotide in the template strand. Under suitable conditions, the nucleotide unit of the nucleotide conjugates binds the primer strand but does not undergo polymerase-catalyzed incorporation. The binding event can be detected, and the specific base of the nucleotide unit can be identified. The nucleotide conjugates described herein are useful for nucleic acid sequencing methods, particularly for massively parallel sequencing methods employed for next gen sequencing platforms.

Abstract: Embodiments of the disclosure provide apparatuses, systems, and methods related to controlling film thickness in photonic-integrated apparatus. In some embodiments, a bottom cladding layer is deposited on a substrate, a thickness map of the bottom cladding layer is generated, thickness trimming is performed on the bottom cladding layer based on the thickness map for the bottom cladding layer; a waveguide core layer is deposited on the bottom cladding layer, a thickness map of the waveguide core layer is generated, thickness trimming is performed on the waveguide core layer based on the thickness map for the bottom waveguide core layer; a top cladding layer is deposited on the waveguide core layer, a thickness map of the top cladding layer is generated; and thickness trimming is performed on the top cladding layer based on the thickness map for the top cladding layer.

Abstract: A signal processing system used for GMR-based detection of a target analyte in a sample under test, comprising: a measurement circuit configuration unit configured to build a GMR sensor measurement circuit by routing in at least one GMR sensor, and to build a reference resistor measurement circuit by routing in at least one reference resistor; a magnetic field excitation unit configured to apply an AC magnetic field of frequency ?2 to the at least one GMR sensor; a carrier signal applying unit configured to apply a carrier signal of frequency ?1 to the GMR sensor measurement circuit, and apply carrier signals of frequency ?1, ?1+?2, and ?1??2 to the reference resistor measurement circuit; a measurement signal pick-up unit coupled to the measurement circuits, configured to collect reference resistor measurement signals from the reference resistor measurement circuit and GMR sensor measurement signals from the GMR sensor measurement circuit; and a phase sensitive solution unit coupled to the measurement signal

Abstract: A method for registering one or more Greenfield devices via a virtual Internet of Things Universal Identifier (IoT UID) is provided. The method includes identifying one or more Brownfield devices, generating device property data based at least in part on the one or more Brownfield devices, and transmitting, to an IoT device registrar server, a registration request that includes the device property data. The method further includes interpreting one or more IoT UIDs generated in response to the transmitting of the registration request.

Abstract: An apparatus for an Internet of Things device registry display includes a user input processing circuit structured to interpret one or more user input command values, an Internet of Things Universal Identification (IoT UID) identification circuit structured to determine one or more IoT UIDs, based at least in part on the one or more user input command values, a device lookup circuit structured to: generate a query that includes the one or more IoT UIDs, and retrieve device property data corresponding to the one or more IoT UIDs, a query provisioning circuit structured to transmit the query to an IoT device registrar server, a device property processing circuit structured to interpret the device property data generated by the IoT device registrar server in response to the query, and a display circuit structured to display the device property data with the corresponding one or more IoT UIDs.

Abstract: A method for determining a trust indicator is provided. The method may include interpreting, via an Internet of Things Universal Identifier (IoT UID) processing circuit, an IoT UID corresponding to a device and identifying, via a record management circuit and based at least in part on the IoT UID, a record in a database corresponding to the device. The method may further include determining, via a trust analysis circuit and based at least in part on the record, a risk indicator of the device and transmitting, via an indicator provisioning circuit, the risk indicator.

Abstract: Rounding hexadecimal floating point numbers using binary incrementors, including: incrementing, by a first incrementor, a first subset of bits of an operand comprising a binary hexadecimal floating point operand; incrementing, by a second incrementor, a second subset of bits of the operand; generate an intermediate result based on a carryout of the second incrementor; and generate an incremented result based on a carryout of the first incrementor and one or more of: a first bit of the intermediate result or the carryout of the second incrementor.

Abstract: A system for detecting analytes in a test sample, and a method for processing the same, is provided. The system includes a cartridge reader unit that has a control unit and a pneumatic system, and a cartridge assembly that prepares the samples with mixing material(s) through communication channels. The assembly has a memory chip with parameters for preparing the sample and at least one sensor. The assembly, pneumatic system, and control unit operate together to prepare the sample and provide the prepared sample to the sensor for detecting analytes, and also process measurements from the sensor to generate test results.

Abstract: In at least one embodiment of the method of operating a laser device (100) having a plurality of laser diodes (1) which can be controlled independently of one another, wherein controlled laser diodes are each operated with an operating current (I), and wherein each laser diode can be operated for a proper operation in a nominal current range (?I), a step A) is carried out in which an input current (I_0) or an input voltage (U_0) is applied to the laser device. Furthermore, a step B) is carried out in which a characteristic value is determined that is representative of a number N of laser diodes that can be operated in the respective nominal current range with the input current applied in step A) or with the input voltage applied in step A). If the characteristic value is representative of N?1, M laser diodes are controlled in a step C) in such a way that the M laser diodes are each operated in the nominal current range, wherein 1?M?N is selected.

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3? modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: An Internet of Things device registry display that includes a user input processing circuit structured to interpret one or more user input command values, an Internet of Things Universal Identification (IoT UID) identification circuit structured to determine one or more IoT UIDs, based at least in part on the one or more user input command values, a device lookup circuit structured to: generate a query that includes the one or more IoT UIDs, and retrieve device property data corresponding to the one or more IoT UIDs, a query provisioning circuit structured to transmit the query to an IoT device registrar server, a device property processing circuit structured to interpret the device property data generated by the IoT device registrar server in response to the query, and a display circuit structured to display the device property data with the corresponding one or more IoT UIDs.

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3? modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: A composite confinement apparatus assembly is provided. The composite confinement apparatus assembly includes a quantum object confinement apparatus and a photonic platform. The confinement apparatus includes one or more electrical components and is fabricated on a confinement apparatus substrate. The photonic platform includes one or more photonic components that are hosted by a photonic platform substrate. The photonic platform substrate is mechanically coupled to the confinement apparatus substrate to form the composite confinement apparatus assembly.

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3? modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: Verifying the correctness of a leading zero counter, including: generating, based on an input value comprising a plurality of digits, a first bit vector, wherein each entry of the first bit vector indicates whether a corresponding digit of the input value is equal to zero; calculating, based on the first bit vector, a leading zero count for the input value; generating a bit mask comprising a number of leading ones equal to the leading zero count; generating a second bit vector comprising a one at a same index as a first occurring zero in the bit mask; and verifying the leading zero count based on the first bit vector and one or more of the bit mask and the second bit vector.