Patents by Inventor Michael R. Knapp
Michael R. Knapp has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8865027Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: GrantFiled: April 4, 2008Date of Patent: October 21, 2014Assignee: Cambrios Technologies CorporationInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Publication number: 20140227709Abstract: Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.Type: ApplicationFiled: April 14, 2014Publication date: August 14, 2014Applicant: CALIPER LIFE SCIENCES, INC.Inventors: Michael R. Knapp, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael Spaid
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Publication number: 20140158400Abstract: A patterned transparent conductor including a conductive layer coated on a substrate is described. More specifically, the transparent conductor has low-visibility patterns.Type: ApplicationFiled: March 8, 2013Publication date: June 12, 2014Inventors: Jeffrey Wolk, Michael R. Knapp
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Patent number: 8697362Abstract: Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.Type: GrantFiled: October 17, 2007Date of Patent: April 15, 2014Assignee: Caliper Life Sciences, Inc.Inventors: Michael R. Knapp, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael A. Spaid
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Patent number: 8618531Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: GrantFiled: December 15, 2010Date of Patent: December 31, 2013Assignee: Cambrios Technologies CorporationInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Publication number: 20120288865Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.Type: ApplicationFiled: April 11, 2012Publication date: November 15, 2012Applicants: Canon U.S. Life Sciences, Inc., Caliper Life Sciences, Inc.Inventors: Steven A. Sundberg, Michael R. Knapp, Ivor T. Knight, Deborah J. Boles, Aaron J. Rulison, Wesley B. Dong, Edward P. Donlon, Robert J. Moti, Andrew G. Fabans, Allen R. Boronkay, Michael Slater
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Patent number: 8275554Abstract: Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.Type: GrantFiled: January 8, 2010Date of Patent: September 25, 2012Assignee: Caliper Life Sciences, Inc.Inventors: Michael R. Knapp, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael A. Spaid
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Publication number: 20110285019Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: ApplicationFiled: December 15, 2010Publication date: November 24, 2011Applicant: Cambrios Technologies CorporationInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Patent number: 8049333Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: GrantFiled: August 14, 2006Date of Patent: November 1, 2011Assignee: Cambrios Technologies CorporationInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Publication number: 20110118139Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.Type: ApplicationFiled: January 27, 2011Publication date: May 19, 2011Applicant: CALIPER LIFE SCIENCES, INC.Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
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Patent number: 7902639Abstract: Improved methods and articles providing conformal coatings for a variety of devices including electronic, semiconductor, and liquid crystal display devices. Peptide formulations which bind to nanoparticles and substrates, including substrates with trenches and vias, to provide conformal coverage as a seed layer. The seed layer can be further enhanced with use of metallic films deposited on the seed layer. Seed layers can be characterized by AFM measurements and improved seed layers provide for better enhancement layers including lower resistivity in the enhancement layer. Peptides can be identified by phage display.Type: GrantFiled: November 16, 2005Date of Patent: March 8, 2011Assignee: Siluria Technologies, Inc.Inventors: Philip E. Garrou, Michael R. Knapp, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid
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Publication number: 20100129896Abstract: Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.Type: ApplicationFiled: January 8, 2010Publication date: May 27, 2010Applicant: CALIPER LIFE SCIENCES, INC.Inventors: MICHAEL R. KNAPP, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael Spaid
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Patent number: 7645581Abstract: Methods for determining nucleic acid fragmentation status are provided. A nucleic acid of interest in a reaction mixture is contacted with two or more different probes complementary to sites separated by a point of potential fragmentation. The probes each comprise a detectable marker. The nucleic acid of interest is flowed into a detection region, where two or more coincident detectable marker signals from the probes are detected. Fragmentation status of the nucleic acid of interest is determined, coincident detection of signals from two or more of the different probes indicating the nucleic acid of interest is not fragmented between the probes.Type: GrantFiled: October 4, 2007Date of Patent: January 12, 2010Assignee: Caliper Life Sciences, Inc.Inventors: Michael R. Knapp, Jill M. Baker, Andrea W. Chow, Anne R. Kopf-Sill, Michael A. Spaid
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Patent number: 7585466Abstract: Genotypes of a subject may be determined by analyzing a biological sample. Measurements of the intensity of allele specific signals may be obtained and used to generate reaction values. These reaction values may be compared to probability distributions in order to obtain conditional probabilities of genotypes of interest. The genotype of the subject may be determined based on the measures of these conditional probabilities.Type: GrantFiled: July 18, 2000Date of Patent: September 8, 2009Assignee: Beckman Coulter, Inc.Inventors: Stephen E. Lincoln, Michael R. Knapp
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Patent number: 7566538Abstract: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3?-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.Type: GrantFiled: January 22, 2008Date of Patent: July 28, 2009Assignee: Caliper Lifesciences Inc.Inventors: J. Wallace Parce, Theo T. Nikiforov, Tammy Burd Mehta, Anne R. Kopf-Sill, Andrea W. Chow, Michael R. Knapp
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Publication number: 20090137413Abstract: Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.Type: ApplicationFiled: October 30, 2007Publication date: May 28, 2009Applicant: CALIPER LIFE SCIENCES, INC.Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
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Publication number: 20080286447Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: ApplicationFiled: April 4, 2008Publication date: November 20, 2008Applicant: Cambrios Technologies CorporationInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Publication number: 20080283799Abstract: A transparent conductor including a conductive layer coated on a substrate is described. More specifically, the conductive layer comprises a network of nanowires which may be embedded in a matrix. The conductive layer is optically transparent and flexible. It can be coated or laminated onto a variety of substrates, including flexible and rigid substrates.Type: ApplicationFiled: April 4, 2008Publication date: November 20, 2008Applicant: CAMBRIOS TECHNOLOGIES CORPORATIONInventors: Jonathan S. Alden, Haixia Dai, Michael R. Knapp, Shuo Na, Hash Pakbaz, Florian Pschenitzka, Xina Quan, Michael A. Spaid, Adrian Winoto, Jeffrey Wolk
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Publication number: 20080131904Abstract: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3?-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.Type: ApplicationFiled: January 22, 2008Publication date: June 5, 2008Applicant: CALIPER LIFE SCIENCES, INC.Inventors: J. Wallace Parce, Theo T. Nikiforov, Tammy Burd Mehta, Anne R. Kopf-Sill, Andrea W. Chow, Michael R. Knapp
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Patent number: 7344865Abstract: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3?-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.Type: GrantFiled: August 11, 2006Date of Patent: March 18, 2008Assignee: Caliper Life Sciences, Inc.Inventors: J. Wallace Parce, Theo T. Nikiforov, Tammy Burd Mehta, Anne R. Kopf-Sill, Andrea W. Chow, Michael R. Knapp