Patents by Inventor Michael R. Slater

Michael R. Slater has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • ENGINEERED DNA POLYMERASE WITH REDUCED ARTIFACT FORMATION
    Publication number: 20240344039
    Abstract: Provided herein are compositions and systems comprising a DNA polymerase domain, a thioredoxin binding domain (TBD), and thioredoxin (TRX), wherein one or both of the TRX and TBD are fused or otherwise conjugated to the DNA polymerase domain. A TRX or TBD may also be provided in a system herein as a separate entity (e.g., a binary system). The DNA polymerase/TBD/TRX compositions and systems herein are engineered to reduce stutter and/or to produce fewer stutter artifacts. Kits comprising the DNA polymerase/TBD/TRX compositions and systems herein and methods of use thereof are also within the scope herein.
    Type: Application
    Filed: March 4, 2024
    Publication date: October 17, 2024
    Inventors: Nicholas A. COURTNEY, Lance P. ENCELL, Robert A. MCLAREN, David Z. MOKRY, Kristopher ZIMMERMAN, Kimberly SCHNELL, Michael KILLORAN, Kimberly K. KNOCHE, Michael R. SLATER, Monika G. WOOD, Michael BROCKMAN
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20240158831
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: September 21, 2023
    Publication date: May 16, 2024
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 11661623
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: September 3, 2020
    Date of Patent: May 30, 2023
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood, Dieter Klaubert, Poncho Meisenheimer, James Unch, Michael P. Valley
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20220348983
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: May 27, 2021
    Publication date: November 3, 2022
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20200399676
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: September 3, 2020
    Publication date: December 24, 2020
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 10774364
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: April 9, 2018
    Date of Patent: September 15, 2020
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Mutant hydrolase proteins with enhanced kinetics and functional expression
    Patent number: 10246690
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Grant
    Filed: January 23, 2018
    Date of Patent: April 2, 2019
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20180327810
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: April 9, 2018
    Publication date: November 15, 2018
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION
    Publication number: 20180223267
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Application
    Filed: January 23, 2018
    Publication date: August 9, 2018
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 9951373
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: April 24, 2018
    Assignee: Promega Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • Mutant dehalogenase proteins
    Patent number: 9873866
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Grant
    Filed: January 24, 2017
    Date of Patent: January 23, 2018
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
    Patent number: 9840730
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: December 12, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION
    Publication number: 20170137793
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Application
    Filed: January 24, 2017
    Publication date: May 18, 2017
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression
    Patent number: 9593316
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: March 14, 2017
    Assignee: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Vectors for directional cloning
    Patent number: 9469857
    Abstract: The invention provides vectors and methods for directional cloning.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: October 18, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Michael R. Slater, James Robert Hartnett
  • Vectors for directional cloning
    Patent number: 9371531
    Abstract: The invention provides vectors and methods for directional cloning.
    Type: Grant
    Filed: January 14, 2013
    Date of Patent: June 21, 2016
    Assignee: PROMEGA CORPORATION
    Inventors: Michael R. Slater, Ethan Edward Strauss, Keith V. Wood, James Robert Hartnett
  • POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION
    Publication number: 20150140636
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Application
    Filed: May 22, 2014
    Publication date: May 21, 2015
    Applicant: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Vectors for directional cloning
    Patent number: 9018014
    Abstract: The invention provides vectors and methods for directional cloning.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: April 28, 2015
    Assignee: Promega Corporation
    Inventors: Michael R. Slater, Keith V. Wood, James Robert Hartnett
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20140227759
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 14, 2014
    Applicant: PROMEGA CORPORATION
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
  • OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE
    Publication number: 20140223590
    Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.
    Type: Application
    Filed: January 21, 2014
    Publication date: August 7, 2014
    Applicant: Promego Corporation
    Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood