Patents by Inventor Michael Seul

Michael Seul has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10415081
    Abstract: The invention provides methods and processes for the identification of polymorphisms at one ore more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one ore more mutations within the CFTR gene and the HLA gene complex can be identified.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: September 17, 2019
    Assignee: BioArray Solutions Ltd.
    Inventors: Alice Xiang Li, Ghazala Hashmi, Michael Seul
  • Patent number: 10407718
    Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: September 10, 2019
    Assignee: BioArray Solutions Ltd.
    Inventors: Michael Seul, Sukanta Banerjee, Jiacheng Yang, Tatiana Vener
  • Publication number: 20190127777
    Abstract: Disclosed is a novel method of allele profiling, or nucleic acid sieving, with pooled Sanger sequencing as a first (aka “screening”) stage; where the first step is: amplifying a single sequence, delineated by forward and reverse primers which may represent a single exon, or a segment thereof, or a contiguous stretch of multiple exons and introns. The amplicons produced from a pool of samples include the amplified sequence, and these are next converted into fragments in the standard Sanger labeling reaction. Ambiguities will appear as superposed peaks at any heterozygous position of interest, as the origin of the variant signal cannot be uniquely attributable to a specific sample, or samples, in the pool. These ambiguities may be resolved by the allele profiling process; or, resolution can be done with source-tagged primers generating source-tagged amplicons, which generate position shifts in labels, which can be decoded to resolve the ambiguities.
    Type: Application
    Filed: November 26, 2017
    Publication date: May 2, 2019
    Inventors: Michael Seul, Ghazala Hashmi
  • Publication number: 20190056409
    Abstract: The invention relates to forming locus-specific (or gene-specific) pools of cells from various potential donors, where the cells that express or display HLA molecules, or other polymorphic cell surface markers, and identifying the pattern of responses across these pools, notably aggregation of cells, or their acquisition of fluorescence when exposed to a labeled secondary antibody, when exposed to sera from a potential recipient. Pools of cells from different potential donors are designed and constructed in accordance with allele partitions that are identified by amino acid sequence tags, preferably defined in terms of single or combinations of variable amino acid positions encoded by the constituent alleles in the partition.
    Type: Application
    Filed: August 20, 2018
    Publication date: February 21, 2019
    Inventor: Michael Seul
  • Patent number: 10208347
    Abstract: A process of identifying a plurality of biological samples having particular desired attributes by testing pooled samples and selecting, for intended uses such as transfusion, or for subsequent analysis that is thereby enriched for such samples, pooled samples which have, or may have, said desired attributes. The preferred number of samples per pool “d” is determined by selecting an integer value as d which produces the maximum or a value near the maximum of the product of: d times the expected number of unambiguous sample pools, where a sample pool is unambiguous if all of the samples have the desired attributes, and is otherwise ambiguous if at least one sample has the desired attributes. The value selected as d can be greater than the maximum product above, so as to enlarge the total number of samples assayed in determining the desired attributes.
    Type: Grant
    Filed: July 5, 2016
    Date of Patent: February 19, 2019
    Assignee: BioInventors & Entrepreneurs Network, LLC
    Inventors: Michael Seul, Ghazala Hashmi
  • Publication number: 20190024156
    Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.
    Type: Application
    Filed: September 25, 2018
    Publication date: January 24, 2019
    Inventors: Michael Seul, Chiu Wo Chau, Hui Huang, Sukanta Banerjee, Jiacheng Yang, Ye Hong
  • Patent number: 10138511
    Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.
    Type: Grant
    Filed: March 29, 2017
    Date of Patent: November 27, 2018
    Assignee: BioArray Solutions Ltd.
    Inventors: Michael Seul, Chiu Wo Chau, Hui Huang, Sukanta Banerjee, Jiacheng Yang, Ye Hong
  • Publication number: 20180218498
    Abstract: Systems and methods are provided the autocentering, autofocusing, acquiring, decoding, aligning, analyzing and exchanging among various parties, images, where the images are of arrays of signals associated with ligand-receptor interactions, and more particularly, ligand-receptor interactions where a multitude of receptors are associated with microparticles or microbeads. The beads are encoded to indicate the identity of the receptor attached, and therefore, an assay image and decoding image are aligned to effect the decoding. The images or data extracted from such images can be exchanged between de-centralized assay locations and a centralized location where the data are analyzed to indicate assay results. Access to data can be restricted to authorized parties in possession of certain encoding information, so as to preserve confidentiality.
    Type: Application
    Filed: March 23, 2018
    Publication date: August 2, 2018
    Applicant: BioArray Solutions, Ltd.
    Inventors: Michael SEUL, Xiongwu XIA, Chiu CHAU, Scott DETERMAN
  • Patent number: 9928587
    Abstract: Systems and methods are provided the autocentering, autofocusing, acquiring, decoding, aligning, analyzing and exchanging among various parties, images, where the images are of arrays of signals associated with ligand-receptor interactions, and more particularly, ligand-receptor interactions where a multitude of receptors are associated with microparticles or microbeads. The beads are encoded to indicate the identity of the receptor attached, and therefore, an assay image and decoding image are aligned to effect the decoding. The images or data extracted from such images can be exchanged between de-centralized assay locations and a centralized location where the data are analyzed to indicate assay results. Access to data can be restricted to authorized parties in possession of certain encoding information, so as to preserve confidentiality.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: March 27, 2018
    Assignee: BioArray Solutions, Ltd.
    Inventors: Michael Seul, Xiongwu Xia, Chiu Chau, Scott Determan
  • Patent number: 9909120
    Abstract: A method of selecting nucleic acid samples including particular desired alleles from a plurality of nucleic acid samples including the steps of performing a first reaction in a plurality of pools containing the samples to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the desired alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products, and further identifying nucleic acid samples with the desired alleles. A source tag sharing number “d” may be determined for each of the alleles. Alleles may also be binned together.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: March 6, 2018
    Assignee: BIOINVENTORS & ENTREPRENEURS NETWORK LLC
    Inventor: Michael Seul
  • Patent number: 9868986
    Abstract: A method of selecting nucleic acid samples including particular desired alleles from a plurality of nucleic acid samples including the steps of performing a first reaction in a plurality of pools containing the samples to produce reaction products including a source tag identifying said each pool; pooling the pools to provide pooled pools; for each of the desired alleles to be identified, performing a second reaction using said reaction products to produce allele-specific second reaction products comprising a marker tag and a derived source tag; identifying said allele-specific second reaction products, and further identifying nucleic acid samples with the desired alleles. A source tag sharing number “d” may be determined for each of the alleles. Alleles may also be binned together.
    Type: Grant
    Filed: April 19, 2015
    Date of Patent: January 16, 2018
    Assignee: BioInventors & Entrepreneurs Network LLC
    Inventor: Michael Seul
  • Publication number: 20170372015
    Abstract: Disclosed is a registry system, including member institutions, in which transfusion donors and recipients are registered following genotyping, which would typically take place in a member institution, or a member institution would have access to the genotyping information, if performed outside. The registry database can be accessed and searched by members seeking samples of particular type(s). Systems are disclosed for maintaining economic viability of genotyping in connection with transfusions, by maximizing the number of units placed with the minimal number of candidate donors typed. Genotyping of potential donors, and product supply, is matched to forecasted demand. Genotyping can also be limited to the more clinically relevant markers. The registry system can also be integrated with one format of assay which generates an image for analysis, whereby the imaged results can be analyzed and redacted by experts in a central location, and then transmitted back to the patient or their representative.
    Type: Application
    Filed: August 10, 2017
    Publication date: December 28, 2017
    Applicant: BioArray Solutions, Ltd.
    Inventors: Michael SEUL, Robert DANEHY
  • Publication number: 20170342489
    Abstract: A process of identifying a plurality of biological samples having particular desired attributes by testing pooled samples and selecting, for intended uses such as transfusion, or for subsequent analysis that is thereby enriched for such samples, pooled samples which have, or may have, said desired attributes. The preferred number of samples per pool “d” is determined by selecting an integer value as d which produces the maximum or a value near the maximum of the product of: d times the expected number of unambiguous sample pools, where a sample pool is unambiguous if all of the samples have the desired attributes, and is otherwise ambiguous if at least one sample has the desired attributes. The value selected as d can be greater than the maximum product above, so as to enlarge the total number of samples assayed in determining the desired attributes.
    Type: Application
    Filed: July 5, 2016
    Publication date: November 30, 2017
    Inventors: Michael Seul, Ghazala Hashmi
  • Publication number: 20170292948
    Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
    Type: Application
    Filed: March 10, 2017
    Publication date: October 12, 2017
    Inventors: Sukanta BANERJEE, Michael SEUL
  • Publication number: 20170268050
    Abstract: In a multiplexed assay method carried out in solution, wherein the solution contains nucleic acid targets and, wherein several different types of oligonucleotide probes, each type having a different sequence in a region designated as a target binding domain, are used to detect the nucleic acid targets, said assay method including a method for increasing the effective concentration of the nucleic acid targets at the surface of a bead to which the oligonucleotide probes are bound, by one or more of the following steps: adjusting assay conditions so as to increase the effective concentration of the targets available for binding to the probes, by one or more of the following: (i) selecting a particular probe density on the surface of the bead; (ii) selecting a solution having an ionic strength greater than a threshold; (ii) selecting a target domain of a size less than a threshold; or (iii) selecting target domains within a specified proximity to a terminal end of the targets.
    Type: Application
    Filed: May 31, 2017
    Publication date: September 21, 2017
    Inventors: Michael SEUL, Yi ZHANG, Sukanta BANERJEE, Jiacheng YANG, Chiu CHAU
  • Publication number: 20170260577
    Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.
    Type: Application
    Filed: March 29, 2017
    Publication date: September 14, 2017
    Inventors: Michael Seul, Chiu Wo Chau, Hui Huang, Sukanta Banerjee, Jiacheng Yang, Ye Hong
  • Publication number: 20170216359
    Abstract: Determining and implementing individualized platelet support regimens (e.g., administering specified platelet numbers; and, schedules for administration or other intervention, in accordance with the requirements assessed for individual patients) by: monitoring platelet clearance kinetics; analyzing the kinetics to determine value(s) proportionally representing both the extent and frequency of vascular injury, and using such value(s) to determine the momentary requirement for maintaining vascular integrity, and constructing a corresponding platelet administration regimen where said regimen preferably provides for the utilization of platelets selected, from a platelet inventory, in accordance with the recipient's molecular “type”. Releasing from an inventory of a platelet inventory system the particular typed or profiled platelet amounts required to satisfy the platelet administration regimen of each patient.
    Type: Application
    Filed: April 17, 2017
    Publication date: August 3, 2017
    Inventors: Michael Seul, Andreas Gocksch
  • Publication number: 20170218437
    Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including: methods of probe and target “engineering”, as well as methods of assay signal analysis relating to the modulation of the probe-target affinity constant, K by a variety of factors including the elastic properties of target strands and layers of immobilized (“grafted”) probes; and assay methodologies relating to: the tuning of assay signal intensities including dynamic range compression and on-chip signal amplification; the combination of hybridization-mediated and elongation-mediated detection for the quantitative determination of abundance of messages displaying a high degree of sequence similarity, including, for example, the simultaneous determination of the relative expression levels, and identification of the specific class of, untranslated AU-rich subsequences located near the 3? terminus of mRNA; and a new method of subtractive differential gene expression analysis which requires only a single color label.
    Type: Application
    Filed: April 19, 2017
    Publication date: August 3, 2017
    Inventors: Michael SEUL, Sukanta Banerjee, Jiacheng YANG, Tatiana Vener
  • Patent number: 9709559
    Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.
    Type: Grant
    Filed: December 28, 2001
    Date of Patent: July 18, 2017
    Assignee: BioArray Solutions, Ltd.
    Inventors: Sukanta Banerjee, Michael Seul
  • Patent number: 9670534
    Abstract: Disclosed herein are methods for array assembly and detection. The methods can use an incubation chamber containing a suspension of nucleic acid targets, polymerase and a set of oligonucleotide probes bound to magnetic beads in a randomly dispersed state. Each probe can have a target binding domain that is complementary to a target nucleic acid, a closing domain with a sequence that is complementary to the sequence of the target binding domain, and a joining region between the binding domain and the closing domain, which is not complementary to the target nucleic acid. Method steps can include providing the incubation chamber, placing the incubation chamber in a magnetic trap, generating a magnetic field that induces the magnetic beads to migrate towards a substrate and, once in proximity to the substrate, to interact with each other repulsively and reorganize into arrays, and imaging the array.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: June 6, 2017
    Assignee: BioArray Solutions, Ltd.
    Inventors: Michael Seul, Yi Zhang, Sukanta Banerjee, Jiacheng Yang, Chiu Chau