Patents by Inventor Michael Wagenbach

Michael Wagenbach has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Hemoglobin-like protein comprising genetically fused globin-like polypeptides
    Patent number: 5844088
    Abstract: The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.We further describe the production of tetrameric human hemoglobin and di-alpha/beta.sub.2 hemoglobin in the yeast Saccharomyces cerevisiae. The synthesis of the protein is directed by a synthetic promotor consisting of two functional parts, an upstream activator sequence (UAS) that confers inducible transcription by galactose from a consensus yeast transcriptional initiation site.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: December 1, 1998
    Assignee: Somatogen, Inc.
    Inventors: Stephen J. Hoffman, Douglas L. Looker, Mary S. Rosendahl, Gary L. Stetler, Michael Wagenbach, David C. Anderson, Antony James Mathews, Kiyoshi Nagai
  • Genetically fused globin-like polypeptides having hemoglobin-like activity
    Patent number: 5844089
    Abstract: The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.
    Type: Grant
    Filed: May 25, 1995
    Date of Patent: December 1, 1998
    Assignee: Somatogen, Inc.
    Inventors: Stephen J. Hoffman, Douglas L. Looker, Mary S. Rosendahl, Gary L. Stetler, Michael Wagenbach, David C. Anderson, Antony James Mathews, Kiyoshi Nagai
  • Co-expression of alpha and beta globins
    Patent number: 5798227
    Abstract: The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.We further describe the production of tetrameric human hemoglobin and di-alpha/beta.sub.2 hemoglobin in the yeast Saccharomyces cerevisiae. The synthesis of the protein is directed by a synthetic promotor consisting of two functional parts, an upstream activator sequence (UAS) that confers inducible transcription by galactose from a consensus yeast transcriptional initiation site.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: August 25, 1998
    Assignee: Somatogen, Inc.
    Inventors: Stephen J. Hoffman, Douglas L. Looker, Gary L. Stetler, Michael Wagenbach
  • DNA encoding fused di-beta globins and production of pseudotetrameric hemoglobin
    Patent number: 5744329
    Abstract: The alpha subunits of hemoglobin, which in nature are formed as separate polypeptide chains which bind noncovalently to the beta subunits, are here provided in the form of the novel molecule di-alpha globin, a single polypeptide chain defined by connecting the two alpha subunits either directly via peptide bond or indirectly by a flexible amino acid or peptide linker. Di-alpha globin may be combined in vivo or in vitro with beta globin and heme to form hemoglobin. Di-alpha globin is expressed by recombinant DNA techniques. Di-beta globin may be similarly obtained.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: April 28, 1998
    Assignee: Somatogen, Inc.
    Inventors: Stephen J. Hoffman, Douglas L. Looker, Mary S. Rosendahl, Gary L. Stetler, Michael Wagenbach, David C. Anderson, Antony James Mathews, Kiyoshi Nagai