Abstract: To enable taking in a biological sample at a position a predetermined distance away from an EWOD electrode, a biochemical cartridge, including a droplet passage on which a plurality of EWOD electrodes is arranged along a direction in which a sample droplet that is a droplet including a biological sample is transported, the plurality of EWOD electrodes configured to transport the sample droplet, and a sample take-in unit having a predetermined distance from an EWOD electrode at a tail end of the droplet passage, the sample take-in unit being provided at a position at which the biological sample in the sample droplet is taken in. When the biological sample is taken in, the sample take-in unit is located at a position lower than the droplet passage having the EWOD electrode at the tail end, and an area between the droplet passage and the sample take-in unit is smoothly connected.

Abstract: Provided is a technique for moving all of a droplet from a microchannel in which the droplet have been introduced to another layer. The droplet transport device of the present disclosure includes a substrate having a through-hole or a recess, a first electrode provided on the substrate along the surface of the substrate and arranged at a position adjacent to the through-hole or the recess, a plurality of second electrodes provided on the substrate along a surface of the substrate and to which a voltage for moving the droplet introduced on the substrate is applied, and a dielectric layer covering the surface of the substrate, the first electrode, and the second electrodes, and a water-repellent film provided on the inner wall surface of the through-hole or the recess, and on the dielectric layer.

Abstract: To introduce a reagent with a small amount of residual liquid and enable a fluidic manipulation by deformation of an elastic film, a sealed type of sample processing device is configured with a reagent storage including a joint portion which joins an upper film and a lower film at a periphery of a storage space which stores a reagent between both films, an analysis chip including a lower surface fluid channel through which a liquid flows on a lower surface side and an upper surface fluid channel through which the liquid flows on an upper surface side, and an elastic film which seals the lower surface side of the analysis chip. A portion of the lower film is joined to the upper surface side of the analysis chip, a removed portion, in which the lower film is partially removed, is in an upper part of the upper surface fluid channel.

Abstract: There is a phenomenon where a noise in a spike shape caused by, for example, impurities and a noise peak having a spectrum different from a wavelength spectrum of a labeled fluorescent substance are detected during a capillary electrophoresis. Therefore, the disclosure provides a technique to identify an intensity of the labeled fluorescent substance itself without an effect by a noise fluorescence peak caused by impurities. In the disclosure, a fluorescence intensity property (a fluorescence profile of a noise) common to the noise peaks is set, the noise peak is handled as a fluorescent substance different from the labeled fluorescent substance, and the fluorescent substance and the noise are separated by color converting with the labeled fluorescent substance+the noise fluorescent substance (see FIG. 5).

Abstract: In order to avoid crosstalk between images by a simple method, an optical system through which lights emitted from a plurality of light emission points pass, and a measurement unit that measures lights imaged by the optical system are provided, and a midpoint of two arbitrary light emission points among the light emission points is shifted away from a center of the optical system. More specifically, the center of the optical system exists outside a light emission point group consisting of the plurality of the light emission points. With the above configuration, main images and ghost images are separated each other, and thus, it is possible to avoid crosstalk caused by ghosting and to correctly analyze emissions from the plurality of the light emission points.

Abstract: In order to avoid crosstalk between images by a simple method, an optical system through which lights emitted from a plurality of light emission points pass, and a measurement unit that measures lights imaged by the optical system are provided, and a midpoint of two arbitrary light emission points among the light emission points is shifted away from a center of the optical system. More specifically, the center of the optical system exists outside a light emission point group consisting of the plurality of the light emission points. With the above configuration, main images and ghost images are separated each other, and thus, it is possible to avoid crosstalk caused by ghosting and to correctly analyze emissions from the plurality of the light emission points.

Abstract: In an electrophoresis apparatus using a capillary, electrophoresis using a single capillary sometimes requires replacement of a sieving matrix. Replacement with a different sieving matrix has conventionally required cleaning with sieving matrix cleaning liquid, which has increased costs and time required. An electrophoresis apparatus according to the present invention comprises an anodic capillary head provided at a distal end of the capillary, a sieving matrix container filled with a sieving matrix used for electrophoresis, and a filling mechanism for filling the capillary with the sieving matrix via the sieving matrix container. The filling mechanism fills the capillary, which is already filled with the sieving matrix, with a sieving matrix different from the already-filled sieving matrix without using sieving matrix cleaning liquid.

Abstract: An apparatus for storing a thin film device, the apparatus including: a thin film device 3 having an insulating thin film containing Si and having a thickness of 100 nm or less; a solution in contact with the thin film; and a container having a tank that seals the solution, wherein the solution is a solution that satisfies any of the following conditions (1) to (3). (1) A solution containing water in a volume ratio of 0% or more to 30% or less (2) A solution cooled and maintained at a temperature equal to or higher than a solidification point and lower than 15° C. (3) A solution that contains a salt with a concentration of 1 mol/L or more and a saturation concentration or less and is cooled and maintained to a temperature equal to or higher than a solidification point and lower than 25° C.

Abstract: The present invention provides a method for constructing a single-stranded nucleic acid molecule for nucleic acid sequencing by means of a nanopore sequencer, said method including: a step in which at least one hairpin primer including a single-stranded region on the 3? side and a pair of primers are used to synthesize a complementary strand of template DNA that includes the target sequence; and a step in which the synthesized complementary strand forms a hairpin structure inside a molecule and a template extension reaction is carried out. The obtained nucleic acid molecule includes both the target sequence and the complementary strand thereof in the sequence. Single strand construction enables analysis by nanopore sequencing, and the sequence of only the target nucleic acid, which does not include information of the complementary strand, is repeatedly analyzed, thus enabling analysis to be conducted with greater precision by addressing the problem of sequence errors.

Abstract: The purpose of the present invention is to provide a method for treating biomolecules and a method for analyzing biomolecules with which it is possible to effectively suppress the clog of nanopores. The present invention is a method for treating biomolecules for analysis in which nanopores are used, wherein the method includes a step for preparing a sample solution that includes ammonium cations represented by a prescribed formula and biomolecules in which at least a portion of the higher-order structure has been fused.

Abstract: Provided are a first tank; a second tank; a thin film having a nanopore, which communicates the first tank to the second tank, and disposed between the first and second tanks; a first electrode provided in the first tank; and a second electrode provided in the second tank. A wall surface of the nanopore has an ion adsorption preventing structure to prevent desorption/adsorption of an ion contained in a solution filling the first tank and/or the second tank, and a voltage is applied between the first and second electrodes to measure an ion current flowing through the nanopore.

Abstract: The purpose of the invention is to provide a method for analyzing biomolecules with which it is possible to easily suppress the occlusion of nanopores. The first embodiment of the invention is a method for analyzing biomolecules including a step for preparing a substrate having nanopores, a step for placing a sample solution including biomolecules and at least one compound selected from the group consisting of primary amines, secondary amines, guanidine compounds, and salts thereof on the substrate, and a step for detecting the changes in light or electrical signal generated when the biomolecules pass through the nanopores.

Abstract: In an apparatus for analyzing small number of samples, wasteful consumption of a polymer as a separation Medium is suppressed. The apparatus has a configuration provided with a plurality of capillary units each of which can be attached/detached to/from the apparatus, and performs analysis for capillaries after sealing of a polymer by attaching the capillaries by the number in accordance with the number of samples. Since analysis can be performed by using capillaries by the number identical with that of the samples to be analyzed, wasteful polymer is not used in the capillaries not used for the analysis. In addition, the polymer injection mechanism is unnecessary and the polymer is not necessary for maintenance and upon starting of analysis. An electrophoresis apparatus which is small in the size with no polymer injection mechanism, and with low running cost not wastefully consuming the polymer can be provided.

Abstract: An object of the present invention is to provide a biomolecule measuring device that can decrease the influence of crosstalk between chambers. A biomolecule measuring device according to the present invention supplies, to electrodes equipped on chambers, voltages modulated differently to each other.

Abstract: A biological sample analyzer using a nanopore, said analyzer comprising: a first chamber that storing a solvent; a baseboard provided with a nanopore through which a biological sample passes; a second chamber which is positioned adjacently to the first chamber via the baseboard and stores the solvent; a first electrode formed in the first chamber; a second electrode formed in the second chamber; a detector detecting the biological sample which has passed through the nanopore; and a stirrer stirring the solvent in the first chamber.

Abstract: Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak.

Abstract: The purpose of the present invention is to control, with a simple structure and high accuracy, irradiation of excitation light to a multi-nanopore substrate without interrupting a measurement. Irradiation of excitation light is performed concurrently to at least one nanopore and at least one reference object on a substrate mounted in an observation container 103. A position irradiated with the excitation light in a measurement sample is calculated on the basis of a signal generated from the reference object detected by a detector 109, and the measurement and a fixed position control is performed concurrently by performing measurement of the measurement object while a drive control part 115 controlling the position of the irradiation of the excitation light to the measurement sample on the basis of the calculation result, whereby an analysis of the measurement sample can be performed in a short time.

Abstract: In the microscope observation container according to the present invention, an observed sample is accommodated by an objective lens barrel provided with a housing extending along the radiation direction of excitation light and an objective lens fixed to an inside surface of the housing. The microscope observation container is provided with a structure for collecting a liquid immersion medium added by dispensation, the structure having a portion contacted by the objective lens barrel during observation. During observation the aforementioned portion is contacted by the objective lens barrel, and the liquid immersion medium is thereby sealed by the objective lens barrel and the structure. The aforementioned portion also has an elastic force, and is deformed so as to conform to the housing of the objective lens by the contact.

Abstract: The present invention provides a method for constructing a single-stranded nucleic acid molecule for nucleic acid sequencing by means of a nanopore sequencer, said method including: a step in which at least one hairpin primer including a single-stranded region on the 3? side and a pair of primers are used to synthesize a complementary strand of template DNA that includes the target sequence; and a step in which the synthesized complementary strand forms a hairpin structure inside a molecule and a template extension reaction is carried out. The obtained nucleic acid molecule includes both the target sequence and the complementary strand thereof in the sequence. Single strand construction enables analysis by nanopore sequencing, and the sequence of only the target nucleic acid, which does not include information of the complementary strand, is repeatedly analyzed, thus enabling analysis to be conducted with greater precision by addressing the problem of sequence errors.

Abstract: With a microspectroscopy device provided with an objective lens with a high numerical aperture, a defocus arises from thermal drift, etc., necessitating auto-focusing. Conventional auto-focus based on through-focus image acquisition takes time, and thus, it cannot be applied to continuous measurement over a long time wherein high-speed sampling is carried out. The present invention addresses this problem by having a defocus-sensing beam that has either defocus or astigmatism fall incident on the objective lens. Since how the image of the spot of the beam for defocus sensing blurs differs depending on the orientation of the defocus, real-time detection of the amount and orientation of defocus becomes possible, and high-speed realtime auto-focus becomes possible.