Patents by Inventor Mikio Niwa
Mikio Niwa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230399368Abstract: The present disclosure provides art that can efficiently introduce a foreign substance of interest from the outside of a eukaryotic cell into at least the cytoplasm of the cell. The herein disclosed method is a method for introducing a foreign substance of interest from the outside of a eukaryotic cell into at least the cytoplasm of the cell, wherein the method comprises: (1) a step of preparing a foreign substance introduction construct that has a carrier peptide fragment comprising MAPRGFSCLLLLTSEIDLPVKRRA (SEQ ID NO: 1) and the foreign substance of interest bonded to the N-terminal side and/or the C-terminal side of the carrier peptide fragment; (2) a step of supplying the foreign substance introduction construct into a sample that contains a target eukaryotic cell; and (3) a step of incubating the sample into which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.Type: ApplicationFiled: October 14, 2021Publication date: December 14, 2023Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Mikio Niwa
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Patent number: 10138469Abstract: Provided are a synthetic peptide that induces the reprogramming of a differentiated cell, a reprogramming-inducing pharmaceutical composition that contains this synthetic peptide, and a method for producing an undifferentiated cell from a differentiated cell using this synthetic peptide. The peptide provided by the present invention is a synthetic peptide having a reprogramming-inducing peptide sequence formed of the amino acid sequence given by SEQ ID NO: 1 or a modified amino acid sequence thereof. The method for producing an undifferentiated cell provided by the present invention includes inducing the reprogramming of a target cell by culturing a cell culture which contains the target cell and to which the synthetic peptide has been supplied.Type: GrantFiled: June 22, 2015Date of Patent: November 27, 2018Assignees: Toagosei Co., Ltd., Keio University, National University Corporation Nagoya UniversityInventors: Tetsuhiko Yoshida, Nahoko Baileykobayashi, Mikio Niwa, Jun Kudo, Tomohiro Izumiyama, Makoto Sawada
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Publication number: 20170233701Abstract: Provided are a synthetic peptide that induces the reprogramming of a differentiated cell, a reprogramming-inducing pharmaceutical composition that contains this synthetic peptide, and a method for producing an undifferentiated cell from a differentiated cell using this synthetic peptide. The peptide provided by the present invention is a synthetic peptide having a reprogramming-inducing peptide sequence formed of the amino acid sequence given by SEQ ID NO: 1 or a modified amino acid sequence thereof. The method for producing an undifferentiated cell provided by the present invention includes inducing the reprogramming of a target cell by culturing a cell culture which contains the target cell and to which the synthetic peptide has been supplied.Type: ApplicationFiled: June 22, 2015Publication date: August 17, 2017Inventors: Tetsuhiko Yoshida, Nahoko Baileykobayashi, Mikio Niwa, Jun Kudo, Tomohiro Izumiyama, Makoto Sawada
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Patent number: 9365618Abstract: This invention provides a TNFR2 expression-inducing composition including as an active ingredient a peptide having TNFR2 expression-inducing activity, and a method for producing cells that express TNFR2 selectively by use of the composition. The cell production method provided by this invention includes: culturing at least one species of cells capable of expressing TNF receptor 2, and supplying the cells with a synthetic peptide consisting of a nuclear localization signal sequence (NLS) or a nucleolar localization signal sequence (NoLS) to enhance TNFR2 expression in the cells.Type: GrantFiled: April 17, 2012Date of Patent: June 14, 2016Assignee: TOAGOSEI CO. LTD.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa
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Patent number: 9309307Abstract: An advantage of some aspects of the invention is to provide an antibody against an amyloid precursor protein signal peptide. The invention provides an amyloid precursor protein signal peptide.Type: GrantFiled: January 6, 2012Date of Patent: April 12, 2016Assignees: SEIKO EPSON CORPORATION, TOAGOSEI CO., LTD.Inventors: Hitoshi Fukushima, Masato Hanamura, Mikio Niwa, Masaji Okamoto, Tomoko Naoe, Tetsuhiko Yoshida
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Patent number: 9133437Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.Type: GrantFiled: January 24, 2014Date of Patent: September 15, 2015Assignee: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
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Publication number: 20140178990Abstract: A method for manufacturing at least one kind of eukaryotic cells or a biosynthetic substance derived from the eukaryotic cells by proliferating the cells, which includes preparing an artificially synthesized peptide for promoting proliferation of the at least one kind of eukaryotic cells, incubating the eukaryotic cells in a culture medium, and adding the synthesized peptide at least once to the culture medium during the incubation process. The synthesized peptide includes an amino acid sequence selected from SEQ ID NOS: 1 to 18, and an amino acid sequence selected from SEQ ID NOS: 19 to 97.Type: ApplicationFiled: January 24, 2014Publication date: June 26, 2014Applicants: JAPAN TISSUE ENGINEERING CO., LTD., TOAGOSEI CO. LTD.Inventors: Tetsuhiko YOSHIDA, Nahoko KOBAYASHI, Mikio NIWA, Chikara SHINOHARA, Masatoki WATANABE, Ken-ichiro HATA
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Patent number: 8673845Abstract: The method for transferring a foreign substance provided by the present invention includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence WRRQARFK (SEQ ID NO. 1) or any amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: GrantFiled: July 28, 2010Date of Patent: March 18, 2014Assignee: Toagosei Co., Ltd.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa, Kenichi Tanaka
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Patent number: 8673846Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.Type: GrantFiled: October 28, 2010Date of Patent: March 18, 2014Assignees: Toagosei Co. Ltd., Japan Tissue Engineering Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
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Publication number: 20140051647Abstract: This invention provides a TNFR2 expression-inducing composition including as an active ingredient a peptide having TNFR2 expression-inducing activity, and a method for producing cells that express TNFR2 selectively by use of the composition. The cell production method provided by this invention includes: culturing at least one species of cells capable of expressing TNF receptor 2, and supplying the cells with a synthetic peptide consisting of a nuclear localization signal sequence (NLS) or a nucleolar localization signal sequence (NoLS) to enhance TNFR2 expression in the cells.Type: ApplicationFiled: April 17, 2012Publication date: February 20, 2014Applicant: TOAGOSEI CO. LTD.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa
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Publication number: 20130345408Abstract: An advantage of some aspects of the invention is to provide an antibody against an amyloid precursor protein signal peptide. The invention provides an amyloid precursor protein signal peptide.Type: ApplicationFiled: January 6, 2012Publication date: December 26, 2013Applicants: TOAGOSEI CO. LTD., SEIKO EPSON CORPORATIONInventors: Hitoshi Fukushima, Masato Hanamura, Mikio Niwa, Masaji Okamoto, Tomoko Naoe, Tetsuhiko Yoshida
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Patent number: 8603967Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO. 1) or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: GrantFiled: July 28, 2010Date of Patent: December 10, 2013Assignee: Toagosei Co., Ltd.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa
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Publication number: 20130323776Abstract: A method for transferring a foreign substance includes: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including any amino acid sequence selected from SEQ ID NOS: 1-6, or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence of the selected sequence identification number, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 8, 2013Publication date: December 5, 2013Inventors: Tetsuhiko YOSHIDA, Nahoko KOBAYASHI, Mikio NIWA, Kenichi TANAKA
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Publication number: 20120208752Abstract: A cell proliferation promoter includes, as an active ingredient, an artificially synthesized peptide that includes (A) an amino acid sequence constituting a membrane-permeable peptide and (B) an amino acid sequence selected from SEQ ID NOs: 19 to 103 or an amino acid sequence formed by substituting, deleting and/or adding one or several amino acid residues in the selected amino acid sequence.Type: ApplicationFiled: October 28, 2010Publication date: August 16, 2012Applicants: JAPAN TISSUE ENGINEERING CO., LTD., TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Chikara Shinohara, Masatoki Watanabe, Ken-ichiro Hata
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Publication number: 20120149053Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including any amino acid sequence selected from SEQ ID Nos. 1, 2, 3, 4, 5, and 6, or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence of the selected sequence identification number, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: June 14, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa, Kenichi Tanaka
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Publication number: 20120122210Abstract: A method for transferring a foreign substance includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence KKRTLRKNDRKKR (SEQ ID NO. 1) or an amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: May 17, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Tetsuhiko Yoshida, Nahoko Kobayashi, Mikio Niwa
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Publication number: 20120122225Abstract: The method for transferring a foreign substance provided by the present invention includes the steps of: preparing a construct for transferring a foreign substance that contains a carrier peptide fragment including either the amino acid sequence WRRQARFK (SEQ ID NO. 1) or any amino acid sequence formed by the substitution, deletion, and/or addition (insertion) of 1, 2, or 3 amino acid residues in the amino acid sequence, and a foreign substance of interest that is bonded to the N-terminus and/or C-terminus of the carrier peptide fragment; supplying the construct for transferring a foreign substance to a test sample that contains a target eukaryotic cell; and incubating the test sample that has been supplied with the construct for transferring a foreign substance to thereby transfer the construct into the eukaryotic cell in the test sample.Type: ApplicationFiled: July 28, 2010Publication date: May 17, 2012Applicant: TOAGOSEI CO. LTD.Inventors: Nahoko Kobayashi, Tetsuhiko Yoshida, Mikio Niwa, Kenichi Tanaka
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Patent number: 7985831Abstract: An epitope peptide is provided, which is capable of binding specifically to an antibody characteristic of Crohn's disease that is specifically observed in patients with Crohn's disease, together with a test method for conveniently and rapidly determining whether a subject is affected by Crohn's disease by use of the above peptide. The epitope peptide to the Crohn's disease antibody comprises a peptide having an amino acid sequences represented by any one of SEQ ID NOS: 1 to 3 or a salt thereof. A test reagent and a test method for Crohn's disease are provided, which comprise the peptide as an active ingredient.Type: GrantFiled: April 26, 2005Date of Patent: July 26, 2011Assignees: Toagosei Co., Ltd., Kurume UniversityInventors: Mikio Niwa, Katsuhiko Matsuo, Keiichi Mitsuyama, Michio Sata
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Patent number: 7594304Abstract: A clamping device (1) for connection in which a housing (4) rotatably accommodates a worm screw (5) to mesh with an array of teeth portion (6) of a strap band (3). The strap band (3) is diametrically contracted upon operating the worm screw (5) to wind the strap band (3) around a connecting portion (2). One end portion of the strap band (3) in which the array of teeth portion (6) resides, is formed into a wavy and concavo-convex configuration in its width direction, thus increasing a meshing area of the array of teeth portion (6) against the worm screw (5). This makes it possible for the strap band (3) to cope with a situation in which the strap band (3) is required to tightly wind the connecting portion (2) with a high tightening force.Type: GrantFiled: April 12, 2004Date of Patent: September 29, 2009Assignee: Takagi Mfg. Co., Ltd.Inventors: Norio Ito, Mikio Niwa
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Publication number: 20090170115Abstract: An epitope peptide is provided, which is capable of binding specifically to an antibody characteristic of Crohn's disease that is specifically observed in patients with Crohn's disease, together with a test method for conveniently and rapidly determining whether a subject is affected by Crohn's disease by use of the above peptide. The epitope peptide to the Crohn's disease antibody comprises a peptide having an amino acid sequences represented by any one of SEQ ID NOS: 1 to 3 or a salt thereof. A test reagent and a test method for Crohn's disease are provided, which comprise the peptide as an active ingredient.Type: ApplicationFiled: April 26, 2005Publication date: July 2, 2009Applicants: TOAGOSEI CO., LTD., KURUME UNIVERSITYInventors: Mikio Niwa, Katsuhiko Matsuo, Keiichi Mitsuyama, Michio Sata