Patents by Inventor Milena OUZUNOVA
Milena OUZUNOVA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12102048Abstract: The present invention relates to non-transgenic and transgenic plants, preferably crop plants, having biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromer histone H3 (CENH3) protein, wherein the polynucleotide comprises at least one mutation causing an alteration of the amino acid sequence of the CENH3 protein, and to a part of the part. Further, the invention provides methods of generating the inducer plants, methods of generating haploid and double haploid plants using the inducer plants as well as methods of facilitating cytoplasm exchange.Type: GrantFiled: October 12, 2020Date of Patent: October 1, 2024Assignee: KWS SAAT SE & CO. KGAAInventors: Christof Bolduan, Frank Breuer, Monika Kloiber-Maitz, Markus Niessen, Milena Ouzunova, Britta Schulz, Silke Wieckhorst
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Patent number: 11895960Abstract: The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the ?2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.Type: GrantFiled: March 31, 2021Date of Patent: February 13, 2024Assignee: KWS SAAT SE & Co. KGaAInventors: Andreas Houben, Raheleh Karimi-Ashiyani, Takayoshi Ishii, Nils Stein, Jochen Kumlehm, Christof Bolduan, Frank Breuer, Monika Kloiber-Maitz, Markus Niessen, Milena Ouzunova, Britta Schulz, Silke Wieckhorst
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Publication number: 20230407415Abstract: The present invention relates to a method for selecting or identifying a B. napus plant having resistance to Phoma stem canker, such as resistance to fungal pathogen(s) L. maculans and/or L. biglobosa, comprising detecting in said B. napus plant, or part thereof (such as tissue or seed), the presence or absence of at least one marker which is on a chromosomal interval between SEQ ID No: 1 and SEQ ID No: 10. The present invention also provides kits of primers for identifying a B. napus plant having resistance to Phoma stem canker, such as resistance to fungal pathogen(s) L. maculans and/or L. biglobosa. Also provided is the use of a marker as defined herein, or a sequence selected from SEQ ID No: 1-10, or a kit of primers according to the present invention to select a B. napus plant having resistance to Phoma stem canker, such as resistance to fungal pathogen(s) L. maculans and/or L. biglobosa.Type: ApplicationFiled: October 26, 2021Publication date: December 21, 2023Applicant: KWS SAAT SE & Co. KGaAInventors: Frank BREUER, Monika KLOIBER-MAITZ, Milena OUZUNOVA, Andreas GERTZ
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Patent number: 11845947Abstract: The present invention provides an improved Helminthosporium turcicum-resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum, a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.Type: GrantFiled: December 16, 2020Date of Patent: December 19, 2023Assignees: KWS SAAT SE & CO. KGAA, UNIVERSITÄT ZÜRICHInventors: Milena Ouzunova, Daniela Scheuermann, Beat Keller, Simon Krattinger, Thomas Wicker, Gerhard Herren, Severine Hurni, Bettina Kessel, Thomas Presterl, Carsten Knaak
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Publication number: 20230279415Abstract: Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.Type: ApplicationFiled: April 18, 2023Publication date: September 7, 2023Applicant: KWS SAAT SE & Co. KGaAInventors: Monika KLOIBER-MAITZ, Silke WIECKHORST, Christof BOLDUAN, Milena OUZUNOVA
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Publication number: 20230279418Abstract: The present invention relates to plants comprising a polynucleic acid encoding a mutated indeterminate gametophyte (ig) protein and a polynucleic acid encoding a mutated centromere or kinetochore protein, wherein said mutated centromere or kinetochore protein preferably is CENH3. The mutated ig and centromere or kinetochore proteins together result in haploid inducing activity, such as in particular paternal haploid inducing activity. The invention further relates to methods for generating such plants and uses thereof.Type: ApplicationFiled: May 28, 2021Publication date: September 7, 2023Applicant: KWS SAAT SE & Co. KGaAInventors: Monika KLOIBER-MAITZ, Christof BOLDUAN, Alevtina RUBAN, Milena OUZUNOVA, Markus NIESSEN
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Publication number: 20230255156Abstract: The present invention relates to maize plants having increased pathogen resistance or tolerance, in particular increased resistance or tolerance to pathogens causing Northern Corn Leaf Blight, i.e. Exserohilum turcicum. Such maize plants are characterized by particular molecular markers The invention further relates to methods for identifying such maize plants.Type: ApplicationFiled: July 14, 2021Publication date: August 17, 2023Applicants: KWS SAAT SE & Co. KGaA, UNIVERSITY OF ZURICHInventors: Daniela SCHEUERMANN, Milena OUZUNOVA, Bettina KESSEL, Beat KELLER, Ping YANG
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Patent number: 11661607Abstract: Sorghum plants are provided which are capable of inducing haploidy by modifications in the genome related to a pollen-specific expressed patatin phospholipid producing haploid offspring and can be produced for hybrid breeding in short time by chromosome doubling inbred lines, that is, homozygous father and mother lines. In addition, methods are provided for producing transgenic and non-transgenic plant haploid inducers and improving the induction performance of plants.Type: GrantFiled: February 28, 2018Date of Patent: May 30, 2023Assignee: KWS SAAT SE & Co. KGaAInventors: Monika Kloiber-Maitz, Silke Wieckhorst, Christof Bolduan, Milena Ouzunova
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Publication number: 20230021982Abstract: The present invention relates to methods for producing plants with increased fungal resistance, preferably seedling resistance against Northern Corn Leaf Blight. Further provided are methods for introducing, modifying, or modulating at least one wall-associated kinase (WAK) in(to) a plant cell, tissue, organ, or whole plant and thereby causing a reduced synthesis of benzoxazinoid and in turn increased fungal resistance. There are further provided methods to identify and/or modify downstream effector molecules in a WAK signalling cascade. Finally, plant cells, tissues, organs or whole plants having increased fungal resistance and methods using substances to activate signalling pathways in a targeted way are provided.Type: ApplicationFiled: August 25, 2022Publication date: January 26, 2023Applicants: KWS SAAT SE & Co. KGaA, UNIVERSITY OF ZURICH, UNIVERSITY OF BERNInventors: Bettina KESSEL, Milena OUZUNOVA, Daniela SCHEUERMANN, Beat KELLER, Simon KRATTINGER, Coraline PRAZ, Ping YANG, Matthias ERB
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Publication number: 20220251592Abstract: The present invention relates to the identification and molecular characterization as well as to the use of genes and markers from a chromosomal interval which has a locus for cold tolerance in maize. The invention further relates to the development of molecular markers for assisting in growth, in particular for preventing a fixing of a “selective sweep” in a region with a low recombination rate, and to the provision of transgenic and non-transgenic plants, in particular maize plants, which show a newly mediated or increased cold tolerance.Type: ApplicationFiled: April 22, 2022Publication date: August 11, 2022Applicant: KWS SAAT SE & CO. KGAAInventors: Milena OUZUNOVA, Thomas PRESTERL, Carsten KNAAK, Daniela SCHEUERMANN, Claude URBANY, Peter WESTHOFF, Elena PESTSOVA, Karin ERNST
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Publication number: 20220243287Abstract: The present invention relates to a QTL allele in maize associated with drought resistance and carbon isotope composition as well as specific marker alleles associated with the QTL allele. The present invention further relates methods for identifying maize plants based on screening for the presence of the QTL allele or marker alleles. The invention also relates to methods for modifying drought resistance and carbon isotope composition in maize plants.Type: ApplicationFiled: May 13, 2020Publication date: August 4, 2022Applicants: KWS SAAT SE & Co. KGaA, TECHNISCHE UNIVERSITAT MUNCHENInventors: Claude URBANY, Milena OUZUNOVA, Thomas PRESTERL, Daniela SCHEUERMANN, Chris-Carolin SCHÖN, Svenja ALTER, Viktoriya AVRAMOVA, Eva BAUER, Sebastian GRESSET
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Patent number: 11345923Abstract: The present invention relates to the identification and molecular characterization as well as to the use of genes and markers from a chromosomal interval which has a locus for cold tolerance in maize. The invention further relates to the development of molecular markers for assisting in growth, in particular for preventing a fixing of a “selective sweep” in a region with a low recombination rate, and to the provision of transgenic and non-transgenic plants, in particular maize plants, which show a newly mediated or increased cold tolerance.Type: GrantFiled: November 26, 2016Date of Patent: May 31, 2022Assignee: KWS SAAT SE & Co. KGaAInventors: Milena Ouzunova, Thomas Presterl, Carsten Knaak, Daniela Scheuermann, Claude Urbany, Peter Westhoff, Elena Pestsova, Karin Ernst
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Publication number: 20220098607Abstract: The present invention relates to plants having the activity of a haploid inducer comprising mutations in certain regions of the KINETOCHORE NULL2 (KNL2) protein in a plant, in particular within the SANTA domain. Provided are methods of generating haploid cells and doubled haploid cells as well as corresponding plants and plant parts. The present invention also relates to a method for identification of a plant in a plant population, wherein the plant has at least one mutation in the KNL2 protein, in particular within the SANTA domain, which confers activity of a haploid inducer. Further provided is a set of oligonucleotides for the identification of a plant having activity of a haploid inducer.Type: ApplicationFiled: January 30, 2020Publication date: March 31, 2022Applicant: KWS SAAT SE & Co. KGaAInventors: Monika KLOIBER-MAITZ, Milena OUZUNOVA, Frank BREUER
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Publication number: 20220049265Abstract: The present invention relates to plants, such as maize, Sorghum or sugar cane, having improved digestibility, in particular improved stover digestibility. The present invention relates to a QTL allele associated with improved digestibility and specific marker alleles associated with the QTL allele. The present invention further relates to such plants, wherein the F35H gene is mutated or wherein F35H expression is altered. The invention also relates to methods for identifying plants having improved digestibility and methods for obtaining such plants.Type: ApplicationFiled: April 24, 2019Publication date: February 17, 2022Applicant: KWS SAAT SE & Co. KGaAInventors: Monika KLOIBER-MAITZ, Therese BOLDUAN, Milena OUZUNOVA, Nina MEYER, Carolina LOPEZ-DURAN
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Publication number: 20210259178Abstract: The present invention relates to non-transgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the ?2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.Type: ApplicationFiled: March 31, 2021Publication date: August 26, 2021Applicant: KWS SAAT SE & Co. KGaAInventors: Andreas HOUBEN, Raheleh KARIMI-ASHIYANI, Takayoshi ISHII, Nils STEIN, Jochen KUMLEHM, Christof BOLDUAN, Frank BREUER, Monika KLOIBER-MAITZ, Markus NIESSEN, Milena OUZUNOVA, Britta SCHULZ, Silke WIECKHORST
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Publication number: 20210137040Abstract: The present invention provides an improved Helminthosporium turcicum-resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum, a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.Type: ApplicationFiled: December 16, 2020Publication date: May 13, 2021Applicants: KWS SAAT SE & CO. KGAA, UNIVERSITÄT ZÜRICHInventors: Milena OUZUNOVA, Daniela SCHEUERMANN, Beat KELLER, Simon KRATTINGER, Thomas WICKER, Gerhard HERREN, Severine HURNI, Bettina KESSEL, Thomas PRESTERL, Carsten KNAAK
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Patent number: 10993391Abstract: The present invention relates to non-tränsgenic and transgenic plants, preferably crop plants, comprising a mutation causing an alteration of the amino acid sequence in the CATD domain of the centromere histone H3 (CENH3), preferably within the loop1 or the ?2-helix of the CATD domain, which have the biological activity of a haploid inducer. Further, the present invention provides methods of generating the plants of the present invention and haploid and double haploid plants obtainable by crossing the plants of the present invention with wildtype plants as well as methods of facilitating cytoplasm exchange.Type: GrantFiled: August 28, 2015Date of Patent: May 4, 2021Assignee: KWS SAAT SE & CO. KGAAInventors: Andreas Houben, Raheleh Karimi-Ashiyani, Takayoshi Ishii, Nils Stein, Jochen Kumlehm, Christof Bolduan, Frank Breuer, Monika Kloiber-Maitz, Markus Niessen, Milena Ouzunova, Britta Schulz, Silke Wieckhorst
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Publication number: 20210079419Abstract: The present invention relates to non-transgenic and transgenic plants, preferably crop plants, having biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromer histone H3 (CENH3) protein, wherein the polynucleotide comprises at least one mutation causing an alteration of the amino acid sequence of the CENH3 protein, and to a part of the part. Further, the invention provides methods of generating the inducer plants, methods of generating haploid and double haploid plants using the inducer plants as well as methods of facilitating cytoplasm exchange.Type: ApplicationFiled: October 12, 2020Publication date: March 18, 2021Applicant: KWS SAAT SE & CO. KGAAInventors: Christof BOLDUAN, Frank BREUER, Monika KLOIBER-MAITZ, Markus NIESSEN, Milena OUZUNOVA, Britta SCHULZ, Silke WIECKHORST
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Publication number: 20210071194Abstract: The present invention relates to a nucleic acid molecule encoding a polypeptide conferring resistance to a plant against a fungal pathogen, such as Helminthosporium turcicum. The present invention further relates to a plant (or part thereof) comprising the nucleic acid molecule, and methods involving the nucleic acid molecule.Type: ApplicationFiled: August 22, 2018Publication date: March 11, 2021Applicants: KWS SAAT SE & Co. KGaA, University of ZurichInventors: Bettina KESSEL, Milena OUZUNOVA, Thomas PRESTERL, Daniela SCHEUERMANN, Gerhard HERREN, Beat KELLER, Simon KRATTINGER, Thomas WICKER, Ping YANG
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Patent number: 10897862Abstract: The present invention provides an improved Helminthosporium turcicum-resistant plant, in particular a maize plant which comprises a polynucleotide with one or more resistance-conferring genes, for example on a truncated chromosome fragment from the accession Pepitilla, as well as a cell, a tissue, a part, grain and seeds thereof, an isolated polynucleotide which comprises one or more resistance-conferring genes against Helminthosporium turcicum, a vector, a transgenic plant cell and a transgenic plant containing this polynucleotide. Furthermore, the invention encompasses suitable markers and their use in introducing resistance or the transgene into a plant, as well as the identification of improved maize plants which comprise a truncated chromosome fragment.Type: GrantFiled: September 3, 2014Date of Patent: January 26, 2021Assignees: KWS SAAT SE & CO. KGAA, UNIVERSITÄT ZÜRICHInventors: Milena Ouzunova, Daniela Scheuermann, Beat Keller, Simon Krattinger, Thomas Wicker, Gerhard Herren, Severine Hurni, Bettina Kessel, Thomas Presterl, Carsten Knaak