Abstract: The present disclosure discloses a design method of a soil cover layer of a solid waste landfill considering the effect of plant root, which relates to the field of designing a soil cover for a solid waste landfill and aims to solve the problem of the prior art that does not consider the non-linear spatial variation of water content and the effect of plant root on the gas migration process. By comprehensively considering the type of plant root architecture, the distribution of water content varying with space, the errors of calculating gas migration caused by assuming that the water content of the cover layer is constant and ignoring the effect of plant root is effectively reduced; and the actual environment of the on-site cover layer is more comprehensively considered, thus improving the calculation accuracy.

Abstract: A method for determining phase locking of critical arc light includes: step 1: monitoring and collecting light radiation intensity of an arc inside a switch cabinet in real time, and converting the collected light radiation intensity into an electrical signal; step 2: extracting a power-frequency fundamental wave of the electrical signal, comparing an amplitude of the power-frequency fundamental wave of the electrical signal with a first threshold, and generating a pre-warning signal based on a comparison result of the first threshold; step 3: comparing the amplitude of the power-frequency fundamental wave of the electrical signal with a second threshold voltage, and generating a control signal based on a comparison result of the second threshold voltage and a protection time threshold; and step 4: protecting the switch cabinet under the critical arc light environment based on the pre-warning signal and the control signal.

Abstract: The automatic door opening and closing device includes: a housing having a mounting space formed therein; and a pushing and rotating assembly provided in the mounting space and having a push rod controlled to be telescopic and a guide rod which extends outwards from the mounting space and is controlled to be movable; wherein the push rod is configured to push open a door of the refrigerator when controlled to extend, and the guide rod is configured to drive the door of the refrigerator to rotate after the push rod pushes open the door of the refrigerator. By integrating the push rod and the guide rod in the mounting space inside the housing.

Abstract: Disclosed are a protective starch-based film and its preparation method and application, belonging to the field of food packaging. According to the method for preparing the protective starch-based film, surfaces of zinc oxide nanoparticles are enabled to adsorb tannic acid and iron ions under the action of metal coordination by controlling the pH to obtain metallic polyphenol network coated zinc oxide nanoparticles, dispersion of the obtained nanoparticles is added to a starch gelatinization solution, and a melanin-like precursor is simultaneously added to form mixed liquid; and by controlling the pH of the mixed solution, ?-? stacking, self-polymerization and metal coordination are induced, so that a stable protective starch-based film with a nano-reinforced interpenetrating network structure is formed.

Abstract: The present disclosure relates to methods of detecting nucleic acid using mass spectrometry. The present disclosure further relates to methods of detecting nucleic acids of and/or diagnosing diseases such as HIV and COVID-19 by detecting presence of target nucleic acid molecule in samples. The present disclosure also relates to kits for detecting nucleic acid and for detecting nucleic acids of and/or diagnosing diseases such as HIV and COVID-19.

Abstract: Disclosed are a protective starch-based film and its preparation method and application, belonging to the field of food packaging. According to the method for preparing the protective starch-based film, surfaces of zinc oxide nanoparticles are enabled to adsorb tannic acid and iron ions under the action of metal coordination by controlling the pH to obtain metallic polyphenol network coated zinc oxide nanoparticles, dispersion of the obtained nanoparticles is added to a starch gelatinization solution, and a melanin-like precursor is simultaneously added to form mixed liquid; and by controlling the pH of the mixed solution, ?-? stacking, self-polymerization and metal coordination are induced, so that a stable protective starch-based film with a nano-reinforced interpenetrating network structure is formed.

Abstract: The present disclosure discloses an antibacterial glucose-based composite nanoparticle and a processing method and use thereof, and belongs to the technical field of processing of modern food. According to the present disclosure, the antibacterial composite nanoparticle is prepared by using a particle surface positioning modification technology and a physical field charge transfer technology with a natural glucose-based nanoparticle as a raw material. The obtained antibacterial composite nanoparticle has a particle size of 50-1,000 nm, a surface zeta potential of 0 to ?10 mV and a broad-spectrum antibacterial rate of greater than 98%. The shelf life of food can be effectively prolonged to prevent spoilage of products. The antibacterial composite nanoparticle can be used in food, textiles, daily chemicals, medicine and many other fields, and has a wide application prospect.

Abstract: The present disclosure discloses an amylopectin-based cyclic glucan and the processing method for the same, and belongs to the technical field of food processing. The present disclosure uses a starch as a raw material, prepares an amylopectin-based cyclic glucan through sugar chain degradation grading and glycosidase-catalyzed trans-glycoside technology, and can be used as a steady-state carrier material for food active factors. The method of the disclosure has advantages of green environmental protection, high processing yield and low cost. The prepared product has high branching degree, special large ring structure and good water solubility, and can be used for steady-state delivery and active protection of natural functional substances, involving nutritional food, medicine, daily chemicals and other fields.

Abstract: Disclosed is a processing method of a self-reinforced starch-based multifunctional material, and belongs to the technical field of starch deep processing. The processing method takes bulk starch as a base material, including the following steps: firstly reacting starch nanoparticles with an organic acid anhydride reagent and adding a bacteriostatic agent to prepare composite nanoparticles, then mixing the composite nanoparticles with the bulk starch, an etherifying agent, a crosslinking agent, a plasticizer and the like, and finally preparing a starch-based multifunctional material by dry extrusion modification combined with a starch-based nanoparticle assembly and reinforcement technology. The method of the disclosure is simple and convenient in step, mild and controllable in reaction, and continuous and green in production.

Abstract: The present disclosure discloses a processing method for intelligent hydrogel from nano-scale starch particles, and belongs to the technical field of nutritional health food. The present disclosure uses dendritic water-soluble starch particles as a skeleton and utilizes a transglycosidation and chain extension-glycan entanglement reaction of glycosyltransferase to obtain an intelligent hydrogel having a spatial reticular structure. The product provided by the present disclosure is an intelligent starch-based hydrogel which has good rehydration capability, biocompatibility, strong gel strength, enzymatic response irreversibility, pH response reversibility, can carry multiple nutritional factors. The hydrogel provided by the present disclosure can protect and control the release of food functional factors, and can be applied to food, biological drug loading, functional materials and the like.

Abstract: The present invention discloses a glucan-based shell-core structure carrier material and preparation and application thereof, and belongs to the technical field of modern food processing. Spherical hyperbranched water-soluble amylum grains are used as the raw material, and an enzymatic grafting and chain extending process is adopted for treatment to modify the surfaces of water-soluble glucan molecules into a firm shell structure with densely cumulated crystal structures, and form the glucan-based carrying material with the shell-core structure of which an inner core cavity has an amorphous state and an outer shell layer has a crystalline state.

Abstract: Disclosed is a processing method of a self-reinforced starch-based multifunctional material, and belongs to the technical field of starch deep processing. The processing method takes bulk starch as a base material, including the following steps: firstly reacting starch nanoparticles with an organic acid anhydride reagent and adding a bacteriostatic agent to prepare composite nanoparticles, then mixing the composite nanoparticles with the bulk starch, an etherifying agent, a crosslinking agent, a plasticizer and the like, and finally preparing a starch-based multifunctional material by dry extrusion modification combined with a starch-based nanoparticle assembly and reinforcement technology. The method of the disclosure is simple and convenient in step, mild and controllable in reaction, and continuous and green in production.

Abstract: The disclosure discloses a feruloyl esterase and application thereof, and belongs to the technical field of microorganisms. The disclosure provides a feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2 and specific enzyme activity as high as 519 U/g. The disclosure provides a recombinant Bacillus subtilis which uses Bacillus subtilis as a host, expresses a gene encoding the feruloyl esterase with an amino acid sequence shown in SEQ ID NO: 2, and can be used for producing the feruloyl esterase with a high yield. The process specifically includes inoculating a fermentation culture medium with the recombinant Bacillus subtilis for fermentation for 14 hours, and the enzyme activity of the feruloyl esterase in a cell disruption supernatant can be as high as 82.53 U/mL.

Abstract: The disclosure discloses a nutritional cereal-based food with low glycemic load and processing method thereof, and belongs to the technical field of processing of healthy foods. According to the disclosure, bulk grain crops are used as raw materials and mixed with a natural plant extract with the content of 3-deoxyanthocyanidin more than 3 mg/100 g and a sulfur-rich plant or animal protein, so that in-situ encapsulation of starch granules is achieved through an interaction of a sulfur-containing protein and 3-deoxyanthocyanidin, and a grain-source nutritional food for special dietary uses with low GI. According to the method of the disclosure, not only is the process environmentally friendly, operation procedures are simple, but also slow release of blood glucose after a meal is achieved by using the product, and the product can be used as a meal replacement food for people with abnormal glucose metabolism and the like.

Abstract: A genetically engineered arginine deiminase reconstructed by site-directed mutagenesis belongs to the technical field of genetic engineering technology. Its amino acid sequence is shown as SEQ ID No. 1. In the amino acid sequence of the arginine deiminase reconstructed by site-directed mutagenesis, glycine at position 264 is mutated to proline, compared to an amino acid sequence of native arginine deiminase. Compared with wild type enzyme, the effective pH range effect of the mutated arginine deiminase according to the present invention is broadened to a certain extent, and especially a good enzyme activity is achieved at physiological pH 7.4. With the broadening of the effective pH effect range, the mutant enzyme still has higher stability under the condition of pH 5.5-7.5.

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C.

Abstract: The present disclosure discloses an amylopectin-based cyclic glucan and the processing method for the same, and belongs to the technical field of food processing. The present disclosure uses a starch as a raw material, prepares an amylopectin-based cyclic glucan through sugar chain degradation grading and glycosidase-catalyzed trans-glycoside technology, and can be used as a steady-state carrier material for food active factors. The method of the disclosure has advantages of green environmental protection, high processing yield and low cost. The prepared product has high branching degree, special large ring structure and good water solubility, and can be used for steady-state delivery and active protection of natural functional substances, involving nutritional food, medicine, daily chemicals and other fields.

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C.

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C.

Abstract: The present disclosure discloses a processing method for intelligent hydrogel from nano-scale starch particles, and belongs to the technical field of nutritional health food. The present disclosure uses dendritic water-soluble starch particles as a skeleton and utilizes a transglycosidation and chain extension-glycan entanglement reaction of glycosyltransferase to obtain an intelligent hydrogel having a spatial reticular structure. The product provided by the present disclosure is an intelligent starch-based hydrogel which has good rehydration capability, biocompatibility, strong gel strength, enzymatic response irreversibility, pH response reversibility, can carry multiple nutritional factors. The hydrogel provided by the present disclosure can protect and control the release of food functional factors, and can be applied to food, biological drug loading, functional materials and the like.