Abstract: The present disclosure provides compositions and methods for binding and/or cleaving a single stranded target nucleic acid. Subject compositions include a Cas9 polypeptide, a guide nucleic acid, and a PAMmer. A subject PAMmer is a single stranded oligonucleotide having a protospacer adjacent motif (PAM) sequence and at least one of: a specifity segment positioned 5? of the PAM sequence, and an orientation segment positioned 3? of the PAM sequence. In some embodiments, the Cas9 polypeptide is a variant Cas9 polypeptide having reduced nuclease activity relative to a corresponding wild type Cas9 polypeptide. In some cases, methods of binding are for visualizing single stranded target nucleic acids using a detectable label. In some cases, methods of binding are for isolating, collecting, and/or analyzing at least one of: (i) bound single stranded target nucleic acids; and (ii) polypeptides associated with bound single stranded target nucleic acids.

Abstract: The present disclosure provides compositions and methods for binding and/or cleaving a single stranded target nucleic acid. Subject compositions include a Cas9 polypeptide, a guide nucleic acid, and a PAMmer. A subject PAMmer is a single stranded oligonucleotide having a protospacer adjacent motif (PAM) sequence and at least one of: a specificity segment positioned 5? of the PAM sequence, and an orientation segment positioned 3? of the PAM sequence. In some embodiments, the Cas9 polypeptide is a variant Cas9 polypeptide having reduced nuclease activity relative to a corresponding wild type Cas9 polypeptide. In some cases, methods of binding are for visualizing single stranded target nucleic acids using a detectable label. In some cases, methods of binding are for isolating, collecting, and/or analyzing at least one of: (i) bound single stranded target nucleic acids; and (ii) polypeptides associated with bound single stranded target nucleic acids.

Abstract: The disclosure relates to a pixel-type biological analysis device comprising a photosensitive layer, a capture mixture for the capture of targeted proteins, the capture mixture being placed on an external surface of the photosensitive layer and comprising a protein probe grafted to a hydrogel, collection means for collecting photoelectrons in the photosensitive layer, and reading and treatment means of an electrical quantity supplied by the collection means, for the supply of a characteristic value of a luminous intensity detected by the photosensitive layer.

Abstract: A method and apparatus for forming raised ridges on the surface of a turbine component having an abradable coating formed on an outer surface thereof which includes a mask having a predetermined pattern of openings therein adjacent the abradable coating on a surface of the turbine component; and a high pressure water jet that has movement relative to the mask so that the high pressure water jet passes along the extent of the openings in the mask and passes through the openings in the mask to remove portions of the abradable coating on the turbine component located beneath the openings in the mask.

Abstract: The disclosure relates to a fabrication process of a biosensor on a semiconductor wafer, comprising steps of: making a central photosensitive zone comprising at least one pixel-type biological analysis device comprising a photosensitive layer, and a first peripheral zone surrounding the central photosensitive zone, comprising electronic circuits. The first peripheral zone is covered by a hydrophilic coating, and the central photosensitive zone is covered with a hydrophobic coating. A barrier of a bio-compatible resin is formed on the second peripheral zone.

Abstract: A method and apparatus for stripping foreign matter from holes in a substrate, such as a turbine component, which includes using a scanner to locate the hole and operating a water jet apparatus to direct a water jet or an abrasive water jet into the hole for stripping. A CAD model of the original turbine component may be used in the control system of the water jet apparatus to initially locate the scanner at the general or theoretical location of the hole. A reverse flow of low pressure water may be provided to further clear foreign matter from the hole.

Abstract: The present invention relates to compositions of matter comprising zinc finger domains that bind to single-stranded RNA and are useful for modifying gene expression such as by regulating processing of messenger RNA (mRNA) or non-coding RNA (ncRNA). The invention also relates to screening, diagnostic and therapeutic methods employing such compositions of matter.

Abstract: A Built-In Test (BIT) for a photoconductive photodiode is performed using the health or characteristics of the photodiode's parasitic capacitance as a “proxy” for the health or characteristics of the photodiode itself. A failure or degradation of the photodiode manifests as a similar failure or degradation of the parasitic capacitance. Under normal operating conditions, the photoconductive photodiode responds to incident photons from a target by generating a photocurrent signal at its cathode. A processor processes the signals from one or more photodiodes to evaluate characteristics of the target. To perform the BIT, a time-varying voltage signal is applied at the photodiode's anode. This signal is coupled through the parasitic capacitance to produce a test current signal at the photodiode's anode. The processor processes the signal to evaluate the health or characteristics of the parasitic capacitance and thus the photodiode.

Abstract: A Built-In Test (BIT) for a photoconductive photodiode is performed using the health or characteristics of the photodiode's parasitic capacitance as a “proxy” for the health or characteristics of the photodiode itself. A failure or degradation of the photodiode manifests as a similar failure or degradation of the parasitic capacitance. Under normal operating conditions, the photoconductive photodiode responds to incident photons from a target by generating a photocurrent signal at its cathode. A processor processes the signals from one or more photodiodes to evaluate characteristics of the target. To perform the BIT, a time-varying voltage signal is applied at the photodiode's anode. This signal is coupled through the parasitic capacitance to produce a test current signal at the photodiode's anode. The processor processes the signal to evaluate the health or characteristics of the parasitic capacitance and thus the photodiode.

Abstract: The disclosure relates to a pixel-type biological analysis device comprising a photosensitive layer, a capture mixture for the capture of targeted proteins, the capture mixture being placed on an external surface of the photosensitive layer and comprising a protein probe grafted to a hydrogel, collection means for collecting photoelectrons in the photosensitive layer, and reading and treatment means of an electrical quantity supplied by the collection means, for the supply of a characteristic value of a luminous intensity detected by the photosensitive layer.

Abstract: The disclosure relates to a fabrication process of a biosensor on a semiconductor wafer, comprising steps of: making a central photosensitive zone comprising at least one pixel-type biological analysis device comprising a photosensitive layer, and a first peripheral zone surrounding the central photosensitive zone, comprising electronic circuits. The first peripheral zone is covered by a hydrophilic coating, and the central photosensitive zone is covered with a hydrophobic coating. A barrier of a bio-compatible resin is formed on the second peripheral zone.

Abstract: A method and apparatus for removing a coating having an irregular surface configuration that includes mapping the irregular surface configuration, and using the mapping determination in controlling the operation of an abrasive water jet to provide increased material removal rates at the higher points in the irregular surface and decreased material removal rates at the lower points, and thereby even out the surface configuration of the coating. The method and apparatus also includes using a detection device for detecting the presence or absence of an element that is unique to a layer of a coating, and using the detection determination in controlling the operation of the abrasive water jet to stop material removal of the coating when the detection device determines there is an absence of the unique element.

Abstract: A method and apparatus for forming raised ridges on the surface of a turbine component having an abradable coating formed on an outer surface thereof which includes a mask having a predetermined pattern of openings therein adjacent the abradable coating on a surface of the turbine component; and a high pressure water jet that has movement relative to the mask so that the high pressure water jet passes along the extent of the openings in the mask and passes through the openings in the mask to remove portions of the abradable coating on the turbine component located beneath the openings in the mask.

Abstract: A method and apparatus for stripping foreign matter from holes in a substrate, such as a turbine component, which includes using a scanner to locate the hole and operating a water jet apparatus to direct a water jet or an abrasive water jet into the hole for stripping. A CAD model of the original turbine component may be used in the control system of the water jet apparatus to initially locate the scanner at the general or theoretical location of the hole. A reverse flow of low pressure water may be provided to further clear foreign matter from the hole.

Abstract: A method and apparatus for removing a coating having an irregular surface configuration that includes mapping the irregular surface configuration, and using the mapping determination in controlling the operation of an abrasive water jet to provide increased material removal rates at the higher points in the irregular surface and decreased material removal rates at the lower points, and thereby even out the surface configuration of the coating. The method and apparatus also includes using a detection device for detecting the presence or absence of an element that is unique to a layer of a coating, and using the detection determination in controlling the operation of the abrasive water jet to stop material removal of the coating when the detection device determines there is an absence of the unique element.

Abstract: A computer implemented internal area access communication system is used for communication between internal applications originating internal communication and running in an internal transaction area on a host computer and external applications originating external communication and running outside of the internal transaction area. Included are an external communication module to convert external communication having formats noncompliant with the internal transaction area, a virtual host terminal to run on the host computer and to execute virtual terminal functions associated with language of the external communication that is noncompliant with the internal transaction area and associated with language compliant with the internal transaction area. Also included is an access state controller to route the external and the internal communication based upon the tracked state information for the internal applications.

Abstract: A computer implemented system controls transaction access of requester applications running on end-user computers having network protocol addresses, to internal applications and their associated transactions running in internal transaction areas of host computer systems. Related to each network protocol address, a requester database contains information related to each network protocol address including end-user identification, possible username and password and instructions, possible priority levels of select transactions, and authorized transactions. A listener listens for a connect request from one of the end-user computers. A validator, using the requester database, determines whether the end-user computer has a valid network protocol address.

Abstract: A front installable guide for a circuit card connecting device is provided. The connecting device has a circuit card connector. The guide has a pair of elongated alignment pins. Each of the pins terminates in a fastener for attachment to the connecting device adjacent the connector. The guide also has a guiding member. The guiding member has a groove which is slidably engagable with an edge of a circuit card. A pair of parallel channels is formed in the guiding member for reception of the alignment pins such that when the alignment pins are received in the channels and attached to the connecting device, the groove is aligned for reception of a circuit card to be connected to the connecting device. The guiding member can provide support for the received circuit card.

Abstract: A computer implemented internal area access communication system is used for communication between internal applications (109) originating internal communication and running in an internal transaction area (108) on a host computer and external applications originating external communication and running outside of the internal transaction area. Included are an external communication module (104) configured to convert external communication having formats noncompliant with the internal transaction area, a virtual host terminal configured to run on the host computer and to execute virtual terminal functions (136) associated with language of the external communication that is noncompliant with the internal transaction area and associated with language compliant with the internal transaction area. Also included is an access state controller (102) configured to route the external and the internal communication based upon the tracked state information for the internal applications.

Abstract: A computer implemented system controls transaction access of requester applications running on end-user computers having network protocol addresses, to internal applications and their associated transactions running in internal transaction areas of host computer systems. Related to each network protocol address, a requester database contains information related to each network protocol address including end-user identification, possible usename and password and instructions, possible priority levels of select transactions, and authorized transactions. A listener listens for a connect request from one of the end-user computers. A validator, using the requester database, determines whether the end-user computer has a valid network protocol address.