Patents by Inventor Nahoko BAILEYKOBAYASHI
Nahoko BAILEYKOBAYASHI has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11965011Abstract: An antitumor peptide provided according to the present invention includes (1) an S1PR-TM related sequence; and (2) an amino acid sequence functioning as a cell penetrating peptide; wherein the total number of amino acid residues is 100 or less.Type: GrantFiled: June 28, 2022Date of Patent: April 23, 2024Assignees: Toagosei Co., LTD, National University Corporation Nagoya UniversityInventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Makoto Sawada
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Patent number: 11927589Abstract: According to the present disclosure, a technology for efficiently introducing a desired foreign substance from the outside of eukaryotic cells into at least the cytoplasm (and the nucleolus) of the cells is provided. A method disclosed here includes a step of preparing a construct for introducing a foreign substance including a carrier peptide fragment composed of any of amino acid sequences: KKRTLRKKKRKKR (SEQ ID NO: 1), KKRTLRKRRRKKR (SEQ ID NO: 2), KKRTLRKRKRKKR (SEQ ID NO: 3) and KKRTLRKKRRKKR (SEQ ID NO: 4), and a foreign substance that is bound to an N-terminal side and/or C-terminal side of the carrier peptide fragment, a step of supplying the construct for introducing a foreign substance to a sample containing desired eukaryotic cells, and a step of incubating the sample to which the construct for introducing a foreign substance is supplied and introducing the construct into eukaryotic cells in the sample.Type: GrantFiled: July 22, 2022Date of Patent: March 12, 2024Assignee: TOAGOSEI CO., LTD.Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Publication number: 20230399368Abstract: The present disclosure provides art that can efficiently introduce a foreign substance of interest from the outside of a eukaryotic cell into at least the cytoplasm of the cell. The herein disclosed method is a method for introducing a foreign substance of interest from the outside of a eukaryotic cell into at least the cytoplasm of the cell, wherein the method comprises: (1) a step of preparing a foreign substance introduction construct that has a carrier peptide fragment comprising MAPRGFSCLLLLTSEIDLPVKRRA (SEQ ID NO: 1) and the foreign substance of interest bonded to the N-terminal side and/or the C-terminal side of the carrier peptide fragment; (2) a step of supplying the foreign substance introduction construct into a sample that contains a target eukaryotic cell; and (3) a step of incubating the sample into which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.Type: ApplicationFiled: October 14, 2021Publication date: December 14, 2023Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Mikio Niwa
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Patent number: 11814448Abstract: The present disclosure provides an artificially synthesized peptide fragment that can efficiently introduce a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell. The present disclosure also provides a method for using the peptide fragment. The herein disclosed peptide fragment is a peptide fragment for introducing a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell, and is provided with a repeat sequence in which the VVRSLVK amino acid sequence (SEQ ID NO: 1) is repeated at least two or more times. By using a construct that has the peptide fragment and the foreign substance of interest bonded to the N-terminal side and/or C-terminal side of the peptide fragment, the foreign substance of interest can be efficiently introduced from outside a eukaryotic cell into at least the cytoplasm of the cell.Type: GrantFiled: November 12, 2021Date of Patent: November 14, 2023Assignee: TOAGOSEI CO., LTD.Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11780903Abstract: The synthetic peptide disclosed herein is a synthetic peptide which is artificially synthesized for suppressing binding of CTLA4 to at least one of B7 proteins B7-1 and B7-2. The synthetic peptide includes a CTLA4-B7 protein binding inhibitory peptide sequence. The CTLA4-B7 protein binding inhibitory peptide sequence is any one of amino acid sequences shown in the following (1) to (3): (1) a TIM3-SP-related sequence; (2) an LAGS-SP-related sequence; and (3) a CTLA4-SP-related sequence. Here, the total number of amino acid residues in the above-described synthetic peptide is 100 or less.Type: GrantFiled: December 9, 2020Date of Patent: October 10, 2023Assignee: TOAGOSEI CO., LTD.Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11767345Abstract: The synthetic peptide disclosed here includes (1) an amino acid sequence represented by any of SEQ ID NOS:1 to 10, or a modified amino sequence formed by deletion, substitution or addition of 1, 2 or 3 amino acid residues in any of these amino acid sequences, together with (2) an amino acid sequence (CPP sequence) that functions as a cell penetrating peptide (CPP), and consists of a total of not more than 100 amino acid residues.Type: GrantFiled: May 25, 2021Date of Patent: September 26, 2023Assignees: TOAGOSEI CO., LTD, National University Corporation Hokkaido UniversityInventors: Tetsuhiko Yoshida, Ayato Takada, Nahoko Baileykobayashi
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Publication number: 20230280333Abstract: The herein disclosed method for introducing a foreign substance of interest from outside a eukaryotic cell into at least a cytoplasm of the cell, comprises: (1) a step of preparing a foreign substance introduction construct that has a carrier peptide fragment comprising either of the amino acid sequences: K K R T L R K S N R K K R W P C (SEQ ID NO: 1) and K K R T L R K K K R K K R W P C (SEQ ID NO: 2), and that has the foreign substance of interest bonded to an N-terminal side and/or C-terminal side of the carrier peptide fragment; (2) a step of supplying the foreign substance introduction construct into a sample that contains a target eukaryotic cell; and (3) a step of incubating the sample into which the foreign substance introduction construct has been supplied, to thereby introduce the construct into the eukaryotic cell in the sample.Type: ApplicationFiled: March 3, 2023Publication date: September 7, 2023Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Publication number: 20230272005Abstract: The herein disclosed art provides amino acid sequence information that can be used to produce antibodies to protein derived from the novel coronavirus (SARS-CoV-2). Synthetic peptide having the herein disclosed amino acid sequence information is recognized as an antigen in at least one species of mammal; contains any of the following amino acid sequences: (1) MKFLVFLGIITTVAA (SEQ ID NO: 1), (2) MFVFLVLLPLVSSQC (SEQ ID NO: 2), and (3) MKIILFLALITLATC (SEQ ID NO: 3); and has a total number of amino acid residues of not more than 20.Type: ApplicationFiled: July 30, 2021Publication date: August 31, 2023Inventors: Tetsuhiko Yoshida, Nahoko Baileykobayashi, Yoshinori Yoshida
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Publication number: 20230160876Abstract: According to the present disclosure, a technology for efficiently introducing a desired foreign substance from the outside of eukaryotic cells into at least the cytoplasm (and the nucleolus) of the cells is provided. A method disclosed here includes a step of preparing a construct for introducing a foreign substance including a carrier peptide fragment composed of any of amino acid sequences: KKRTLRKKKRKKR (SEQ ID NO: 1), KKRTLRKRRRKKR (SEQ ID NO: 2), KKRTLRKRKRKKR (SEQ ID NO: 3) and KKRTLRKKRRKKR (SEQ ID NO: 4), and a foreign substance that is bound to an N-terminal side and/or C-terminal side of the carrier peptide fragment, a step of supplying the construct for introducing a foreign substance to a sample containing desired eukaryotic cells, and a step of incubating the sample to which the construct for introducing a foreign substance is supplied and introducing the construct into eukaryotic cells in the sample.Type: ApplicationFiled: July 22, 2022Publication date: May 25, 2023Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11603395Abstract: A synthetic peptide provided according to the technology disclosed here includes (1) a CMTM4-TM related sequence; and (2) an amino acid sequence that functions as a cell membrane permeable peptide. The synthetic peptide has a total number of amino acid residues of 100 or less.Type: GrantFiled: June 1, 2020Date of Patent: March 14, 2023Assignee: TOAGOSEI CO., LTDInventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Publication number: 20230047473Abstract: The present disclosure provides siRNA that suppresses proliferation of new coronaviruses (SARS-CoV-2). The siRNA disclosed herein includes: a sense strand; and an antisense strand. The sense strand includes a target sequence comprising 19 to 23 bases in which a base at a 5? terminal is guanine (G) or cytosine (C), and an overhang comprising 2 to 4 bases added to a 3? terminal side of the target sequence. The antisense strand includes a sequence complementary to the target sequence, and an overhang comprising 2 to 4 bases added to a 3? terminal side of the complementary sequence. Here, at least a part of the target sequence contains at least a part of a base sequence encoding a signal peptide region of a spike protein (S protein) of SARS-CoV-2.Type: ApplicationFiled: July 12, 2022Publication date: February 16, 2023Inventors: Tetsuhiko Yoshida, Nahoko Baileykobayashi, Kazuo Takayama, Rina Hashimoto, Yoshinori Yoshida
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Publication number: 20220411480Abstract: An antitumor peptide provided according to the present invention includes (1) an S1PR-TM related sequence; and (2) an amino acid sequence functioning as a cell penetrating peptide; wherein the total number of amino acid residues is 100 or less.Type: ApplicationFiled: June 28, 2022Publication date: December 29, 2022Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Makoto Sawada
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Patent number: 11505589Abstract: An antitumor peptide provided according to the present invention includes (1) an S1PR-TM related sequence; and (2) an amino acid sequence functioning as a cell penetrating peptide; wherein the total number of amino acid residues is 100 or less.Type: GrantFiled: October 28, 2019Date of Patent: November 22, 2022Assignees: TOAGOSEI CO., LTD, National University Corporation Nagoya UniversityInventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Makoto Sawada
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Patent number: 11472845Abstract: A synthetic peptide provided by the present invention includes: (1) a CTLA4-SP-related sequence; and (2) an amino acid sequence that functions as a cell penetrating peptide, wherein the synthetic peptide has a total number of amino acid residues of 100 or less.Type: GrantFiled: October 8, 2020Date of Patent: October 18, 2022Assignees: TOAGOSEI CO., LTD, The University of TokyoInventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Shuichi Asakawa
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Publication number: 20220242916Abstract: The present disclosure provides a synthetic peptide containing an amino acid sequence that can be used to produce antibodies against proteins derived from a new coronavirus (SARS-CoV-2). The synthetic peptide disclosed herein is recognized as an antigen relative to at least one mammal, contains an amino acid sequence which is LNESLIDLQELGKYEQYIKWP (SEQ ID No: 1), and has a total number of amino acid residues of 25 or less.Type: ApplicationFiled: January 18, 2022Publication date: August 4, 2022Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11359180Abstract: Provided is a method for producing myocardial cells from pluripotent stem cells. The myocardial cell production method provided by the present invention includes supplying an artificially produced synthetic peptide to a cell culture that contains pluripotent stem cells. The synthetic peptide is a peptide that contains a myocardial cell differentiation-inducing peptide sequence that induces pluripotent stem cells into myocardial cells. The myocardial cell differentiation-inducing peptide sequence is an amino acid sequence selected from the group consisting of (i) an amino acid sequence constituting the signal peptide of any protein belonging to the amyloid precursor protein (APP) family, (ii) a partial amino acid sequence of the amino acid sequence according to (i), and (iii) a modified amino acid sequence from the amino acid sequence according to (i) or (ii).Type: GrantFiled: April 28, 2016Date of Patent: June 14, 2022Assignees: Toagosei Co., Ltd., Kyoto UniversityInventors: Tetsuhiko Yoshida, Kenichi Tanaka, Nahoko Baileykobayashi, Yoshinori Yoshida
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Patent number: 11352408Abstract: Provide is an artificially synthesized antitumor peptide that may suppress proliferation of tumor cells. The peptide provided is a synthetic peptide that contains both (1) an amino acid sequence that forms a signal peptide of a membrane protein, programmed cell death-1 (PD-1), or a modified amino acid sequence thereof in which 1, 2 or 3 amino acid residues deleted from, substituted in or added to the above amino acid sequence; and (2) an amino acid sequence that serves as a cell penetrating peptide (CPP), and wherein the synthetic peptide comprises a total of 100 or fewer amino acid residues.Type: GrantFiled: March 27, 2018Date of Patent: June 7, 2022Assignees: Toagosei Co., Ltd., National University Corporation Nagoya UniversityInventors: Makoto Sawada, Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11339203Abstract: The antitumor peptide provided by the present invention is a synthetic peptide including both of: (1) an amino acid sequence composing the second transmembrane region from the N-terminal of a membrane protein sphingosine 1-phosphate receptor 1 (S1PR1), or a modified amino acid sequence formed by deletion substitution or addition of 1, 2 or 3 amino acid residues in the amino acid sequence; and (2) an amino acid sequence functioning as a cell-penetrating peptide (CPP); wherein the total number of amino acid residues is 100 or less.Type: GrantFiled: July 30, 2019Date of Patent: May 24, 2022Assignees: Toagosei Co., Ltd., National University Corporation Nagoya UniversityInventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida, Makoto Sawada
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Publication number: 20220153784Abstract: The present disclosure provides an artificially synthesized peptide fragment that can efficiently introduce a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell. The present disclosure also provides a method for using the peptide fragment. The herein disclosed peptide fragment is a peptide fragment for introducing a foreign substance of interest from outside a eukaryotic cell into at least the cytoplasm of the cell, and is provided with a repeat sequence in which the VVRSLVK amino acid sequence (SEQ ID NO: 1) is repeated at least two or more times. By using a construct that has the peptide fragment and the foreign substance of interest bonded to the N-terminal side and/or C-terminal side of the peptide fragment, the foreign substance of interest can be efficiently introduced from outside a eukaryotic cell into at least the cytoplasm of the cell.Type: ApplicationFiled: November 12, 2021Publication date: May 19, 2022Inventors: Nahoko Baileykobayashi, Tetsuhiko Yoshida
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Patent number: 11324831Abstract: Provided is a novel material used to introduce an exogenous substance into cells. Also provided is a method for introducing an exogenous substance into target cells using this material. The present invention provides an exosome that is used to introduce an exogenous substance into target cells, wherein the exosome contains one type or two or more types of an exogenous substance and a substance that induces macropinocytosis in the target cells. The present invention also provides a composition containing the exosome and a method for introducing an exogenous substance into cells using this exosome.Type: GrantFiled: May 3, 2017Date of Patent: May 10, 2022Inventors: Ikuhiko Nakase, Tetsuhiko Yoshida, Nahoko Baileykobayashi