Abstract: A liquid reagent of a stabilized thyroid hormone-immobilized carrier, by which a thyroid hormone can be measured easily in a short time and at low cost, is provided. The liquid reagent of a thyroid hormone-immobilized carrier according to the present invention includes: a thyroid hormone-immobilized carrier; and a solvent, and a pH of the solvent containing the thyroid hormone-immobilized carrier is in a range from 8.7 to 11.5. The detection of a thyroid hormone using the liquid reagent of the present invention can be carried out by competitively binding a thyroid hormone in a sample and the thyroid hormone-immobilized carrier in the liquid reagent with an anti-thyroid hormone antibody and detecting a composite of the thyroid hormone-immobilized carrier and the anti-thyroid hormone antibody.

Abstract: Provided are a method of detecting a target with high accuracy, a method of suppressing an increase in background, and a detection apparatus. A first target-binding substance that is immobilized at a carrier and is capable of binding to a target and a second target-binding substance that supports a labeling substance and is capable of binding to the target are used. By reacting a sample, the first target-binding substance, and the second target-binding substance, a complex of the target in the sample, the first target-binding substance, and the second target-binding substance is formed in a first container. Subsequently, after the complex is transferred from the first container to a second container, the complex is detected by detecting the labeling substance in the second container.